PMID- 34927092
OWN - NLM
STAT- MEDLINE
DCOM- 20220405
LR  - 20220405
IS  - 2666-1667 (Electronic)
IS  - 2666-1667 (Linking)
VI  - 2
IP  - 4
DP  - 2021 Dec 17
TI  - A macrophage-T cell coculture model for severe tissue injury-induced T cell 
      death.
PG  - 100983
LID - 10.1016/j.xpro.2021.100983 [doi]
LID - 100983
AB  - A key observation of tissue injury, such as stroke and burn, is a state of 
      systemic immunosuppression characterized by loss of T cells and rise of 
      infections. Here, we present an in vitro model for cell-cell interactions between 
      innate (macrophages) and adaptive (T cells) immune cells. This protocol 
      facilitates bone marrow-derived macrophages (BMDMs) and splenic T cells in a 
      coculture model. The procedure mimics injury-induced T cell death, which is 
      driven by inflammasome activation in macrophages. For complete details on the use 
      and execution of this protocol, please refer to Roth et al. (2021).
CI  - (c) 2021 The Authors.
FAU - Zhu, Jie
AU  - Zhu J
AD  - Institute for Stroke and Dementia Research (ISD), University Hospital, LMU 
      Munich, Munich, Germany.
FAU - Cao, Jiayu
AU  - Cao J
AD  - Institute for Stroke and Dementia Research (ISD), University Hospital, LMU 
      Munich, Munich, Germany.
FAU - Liesz, Arthur
AU  - Liesz A
AD  - Institute for Stroke and Dementia Research (ISD), University Hospital, LMU 
      Munich, Munich, Germany.
AD  - Munich Cluster for Systems Neurology (SyNergy), Munich, Germany.
FAU - Roth, Stefan
AU  - Roth S
AD  - Institute for Stroke and Dementia Research (ISD), University Hospital, LMU 
      Munich, Munich, Germany.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20211201
PL  - United States
TA  - STAR Protoc
JT  - STAR protocols
JID - 101769501
RN  - 0 (Inflammasomes)
SB  - IM
MH  - Animals
MH  - Cell Death/*physiology
MH  - Coculture Techniques/*methods
MH  - Female
MH  - Inflammasomes/immunology/metabolism
MH  - Macrophages/*cytology
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - *Models, Immunological
MH  - Spleen/cytology
MH  - T-Lymphocytes/*cytology
MH  - Wounds and Injuries/immunology/metabolism
PMC - PMC8646263
OTO - NOTNLM
OT  - Cell Biology
OT  - Cell culture
OT  - Cell isolation
OT  - Health Sciences
OT  - Immunology
COIS- The authors declare no competing interests.
EDAT- 2021/12/21 06:00
MHDA- 2022/04/06 06:00
PMCR- 2021/12/01
CRDT- 2021/12/20 06:26
PHST- 2021/12/20 06:26 [entrez]
PHST- 2021/12/21 06:00 [pubmed]
PHST- 2022/04/06 06:00 [medline]
PHST- 2021/12/01 00:00 [pmc-release]
AID - S2666-1667(21)00689-4 [pii]
AID - 100983 [pii]
AID - 10.1016/j.xpro.2021.100983 [doi]
PST - epublish
SO  - STAR Protoc. 2021 Dec 1;2(4):100983. doi: 10.1016/j.xpro.2021.100983. eCollection 
      2021 Dec 17.