PMID- 34927189
OWN - NLM
STAT- MEDLINE
DCOM- 20220308
LR  - 20220308
IS  - 1473-0189 (Electronic)
IS  - 1473-0189 (Linking)
VI  - 22
IP  - 2
DP  - 2022 Jan 18
TI  - Exhausted mature dendritic cells exhibit a slower and less persistent random 
      motility but retain chemotaxis against CCL19.
PG  - 377-386
LID - 10.1039/d1lc00876e [doi]
AB  - Dendritic cells (DCs), which are immune sentinels in the peripheral tissues, play 
      a number of roles, including patrolling for pathogens, internalising antigens, 
      transporting antigens to the lymph nodes (LNs), interacting with T cells, and 
      secreting cytokines. The well-coordinated migration of DCs under various 
      immunological or inflammatory conditions is therefore essential to ensure an 
      effective immune response. Upon maturation, DCs migrate faster and more 
      persistently than immature DCs (iDCs), which is believed to facilitate 
      CCR7-dependent chemotaxis. It has been reported that lipopolysaccharide-activated 
      DCs produce IL-12 only transiently, and become resistant to further stimulation 
      through exhaustion. However, little is known about the influence of DC exhaustion 
      on cellular motility. Here, we studied the cellular migration of exhausted DCs in 
      tissue-mimicked confined environments. We found that the speed of exhausted 
      matured DCs (xmDCs) decreased significantly compared to active matured DCs 
      (amDCs) and iDCs. In contrast, the speed fluctuation increased compared to that 
      of amDCs and was similar to that of iDCs. In addition, the diffusivity of the 
      xmDCs was significantly lower than that of the amDCs, which implies that DC 
      exhaustion reduces the space exploration ability. Interestingly, CCR7-dependent 
      chemotaxis against CCL19 in xmDCs was not considerably different from that 
      observed in amDCs. Taken together, we report a unique intrinsic cell migration 
      behaviour of xmDCs, which exhibit a slower, less persistent, and less diffusive 
      random motility, which results in the DCs remaining at the site of infection, 
      although a well-preserved CCR7-dependent chemotactic motility is maintained.
FAU - Choi, Yongjun
AU  - Choi Y
AUID- ORCID: 0000-0002-3814-3643
AD  - Department of Biomedical Engineering, Ulsan National Institute of Science and 
      Technology (UNIST), Ulsan 44919, Republic of Korea. ykcho@unist.ac.kr.
AD  - Center for Soft and Living Matter, Institute for Basic Science (IBS), Ulsan 
      44919, Republic of Korea.
FAU - Sunkara, Vijaya
AU  - Sunkara V
AD  - Center for Soft and Living Matter, Institute for Basic Science (IBS), Ulsan 
      44919, Republic of Korea.
FAU - Lee, Yeojin
AU  - Lee Y
AD  - Department of Biomedical Engineering, Ulsan National Institute of Science and 
      Technology (UNIST), Ulsan 44919, Republic of Korea. ykcho@unist.ac.kr.
AD  - Center for Soft and Living Matter, Institute for Basic Science (IBS), Ulsan 
      44919, Republic of Korea.
FAU - Cho, Yoon-Kyoung
AU  - Cho YK
AUID- ORCID: 0000-0001-6423-1834
AD  - Department of Biomedical Engineering, Ulsan National Institute of Science and 
      Technology (UNIST), Ulsan 44919, Republic of Korea. ykcho@unist.ac.kr.
AD  - Center for Soft and Living Matter, Institute for Basic Science (IBS), Ulsan 
      44919, Republic of Korea.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20220118
PL  - England
TA  - Lab Chip
JT  - Lab on a chip
JID - 101128948
RN  - 0 (Cytokines)
RN  - 0 (Receptors, CCR7)
SB  - IM
MH  - Cell Movement
MH  - *Chemotaxis
MH  - Cytokines
MH  - *Dendritic Cells
MH  - Receptors, CCR7
EDAT- 2021/12/21 06:00
MHDA- 2022/03/09 06:00
CRDT- 2021/12/20 06:29
PHST- 2021/12/21 06:00 [pubmed]
PHST- 2022/03/09 06:00 [medline]
PHST- 2021/12/20 06:29 [entrez]
AID - 10.1039/d1lc00876e [doi]
PST - epublish
SO  - Lab Chip. 2022 Jan 18;22(2):377-386. doi: 10.1039/d1lc00876e.