PMID- 34928287
OWN - NLM
STAT- MEDLINE
DCOM- 20220225
LR  - 20220225
IS  - 2042-650X (Electronic)
IS  - 2042-6496 (Linking)
VI  - 13
IP  - 2
DP  - 2022 Jan 24
TI  - Identification of mitochondria-related hub genes in sarcopenia and functional 
      regulation of MFG-E8 on ROS-mediated mitochondrial dysfunction and cell cycle 
      arrest.
PG  - 624-638
LID - 10.1039/d1fo02610k [doi]
AB  - Sarcopenia has high prevalence in the elderly population, but the genes and 
      pathways related to aging in elderly patients with sarcopenia are poorly 
      understood. Milk fat globule epidermal growth factor 8 (MFG-E8) is a peripheral 
      membrane glycoprotein isolated from the milk fat globule membrane (MFGM). It has 
      been found to exhibit various nutritional effects, including antibacterial, 
      anti-cancer, anti-oxidant, anti-sarcopenia, and improving brain development and 
      cognitive effects. This study aimed to investigate key differentially expressed 
      genes (DEGs) and pathways associated with the progression of sarcopenia using 
      bioinformatics analysis and in vitro myoblast experiment. The gene expression 
      profiles of GSE8479 and GSE9676, which includes 40 young normal samples and 55 
      elderly samples, were downloaded from the Gene Expression Omnibus Database (GEO). 
      Over 3253 DEGs were identified in the young and elderly samples (adjusted p value 
      <0.05). A total of 213 co-expressed significantly DEGs were identified with Venn 
      diagrams, including 82 up-regulated DEGs and 131 down-regulated DEGs. Based on 
      the analysis of Gene Ontology (GO), protein-protein interaction (PPI) networks 
      and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway, 10 hub genes screened 
      by our study have been proved to play a role in regulating the occurrence and 
      development of aging-related sarcopenia mainly via metabolic pathways, 
      Huntington's disease, Parkinson's disease, oxidative phosphorylation and 
      non-alcoholic fatty liver disease pathways. To further verify the protective 
      effect of MFG-E8 on oxidative stress injured myoblasts, the cell cycle 
      distribution, cell viability and reactive oxygen species (ROS) production were 
      measured. The protein and mRNA levels of Akt, extracellular regulated protein 
      kinases (ERK), p21Cip1, p27Kip1, cyclin D1, cyclin E1, cyclin-dependent kinase 
      (CDK) 2 and 4 were quantified using qRT-PCR and western blot analysis. The 
      results indicated that MFG-E8 has potential anti-sarcopenia effects by promoting 
      ERK and Akt activation-mediated cell proliferation and cell cycle progression in 
      myoblasts.
FAU - Li, He
AU  - Li H
AUID- ORCID: 0000-0001-9345-042X
AD  - College of Health Sciences, Jiangsu Normal University, Xuzhou 221116, Jiangsu, 
      P.R. China. lhe400080@sina.com.
AD  - Jiangsu Province Engineering Research Center of Cardiovascular Drugs Targeting 
      Endotheial Cells, Xuzhou 221116, Jiangsu, P.R. China.
FAU - Guan, Kaifang
AU  - Guan K
AUID- ORCID: 0000-0003-0438-7356
AD  - College of Health Sciences, Jiangsu Normal University, Xuzhou 221116, Jiangsu, 
      P.R. China. lhe400080@sina.com.
AD  - School of Chemistry and Chemical Engineering, Harbin Institute of Technology, 
      Harbin 150090, Heilongjiang, P.R. China.
FAU - Liu, DanDan
AU  - Liu D
AD  - College of Health Sciences, Jiangsu Normal University, Xuzhou 221116, Jiangsu, 
      P.R. China. lhe400080@sina.com.
FAU - Liu, Min
AU  - Liu M
AD  - School of Chemistry and Chemical Engineering, Guangxi University for 
      Nationalities, Nanning 530008, Guangxi, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20220124
PL  - England
TA  - Food Funct
JT  - Food & function
JID - 101549033
RN  - 0 (Antigens, Surface)
RN  - 0 (MFGE8 protein, human)
RN  - 0 (Milk Proteins)
RN  - 0 (Reactive Oxygen Species)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - *Antigens, Surface/genetics/metabolism
MH  - Cell Cycle Checkpoints/genetics
MH  - Computational Biology
MH  - Humans
MH  - *Milk Proteins/genetics/metabolism
MH  - *Mitochondria/genetics/metabolism/pathology
MH  - Protein Interaction Maps/*genetics
MH  - Reactive Oxygen Species/*metabolism
MH  - *Sarcopenia/genetics/metabolism
MH  - Transcriptome/genetics
MH  - Young Adult
EDAT- 2021/12/21 06:00
MHDA- 2022/02/26 06:00
CRDT- 2021/12/20 12:20
PHST- 2021/12/21 06:00 [pubmed]
PHST- 2022/02/26 06:00 [medline]
PHST- 2021/12/20 12:20 [entrez]
AID - 10.1039/d1fo02610k [doi]
PST - epublish
SO  - Food Funct. 2022 Jan 24;13(2):624-638. doi: 10.1039/d1fo02610k.