PMID- 34930951
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20240404
IS  - 2057-3995 (Electronic)
IS  - 2057-3995 (Linking)
VI  - 6
IP  - 1
DP  - 2021 Dec 20
TI  - A bioinspired gelatin-hyaluronic acid-based hybrid interpenetrating network for 
      the enhancement of retinal ganglion cells replacement therapy.
PG  - 85
LID - 10.1038/s41536-021-00195-3 [doi]
LID - 85
AB  - Biomaterial-based cell replacement approaches to regenerative medicine are 
      emerging as promising treatments for a wide array of profound clinical problems. 
      Here we report an interpenetrating polymer network (IPN) composed of 
      gelatin-hydroxyphenyl propionic acid and hyaluronic acid tyramine that is able to 
      enhance intravitreal retinal cell therapy. By tuning our bioinspired hydrogel to 
      mimic the vitreous chemical composition and mechanical characteristics we were 
      able to improve in vitro and in vivo viability of human retinal ganglion cells 
      (hRGC) incorporated into the IPN. In vivo vitreal injections of cell-bearing IPN 
      in rats showed extensive attachment to the inner limiting membrane of the retina, 
      improving with hydrogels stiffness. Engrafted hRGC displayed signs of 
      regenerating processes along the optic nerve. Of note was the decrease in the 
      immune cell response to hRGC delivered in the gel. The findings compel further 
      translation of the gelatin-hyaluronic acid IPN for intravitreal cell therapy.
CI  - (c) 2021. The Author(s).
FAU - Dromel, Pierre C
AU  - Dromel PC
AUID- ORCID: 0000-0002-8426-4489
AD  - Massachusetts Institute of Technology, Cambridge, MA, USA.
AD  - Schepens Eye Research Institute of Massachusetts Eye and Ear, Harvard Medical 
      School, Boston, MA, USA.
FAU - Singh, Deepti
AU  - Singh D
AD  - Schepens Eye Research Institute of Massachusetts Eye and Ear, Harvard Medical 
      School, Boston, MA, USA.
FAU - Andres, Eliot
AU  - Andres E
AD  - Schepens Eye Research Institute of Massachusetts Eye and Ear, Harvard Medical 
      School, Boston, MA, USA.
FAU - Likes, Molly
AU  - Likes M
AD  - Wellesley College, Wellesley, MA, USA.
FAU - Kurisawa, Motoichi
AU  - Kurisawa M
AD  - A*STAR Institute of Bioengineering and Nanotechnology, Singapore, Singapore.
FAU - Alexander-Katz, Alfredo
AU  - Alexander-Katz A
AD  - Massachusetts Institute of Technology, Cambridge, MA, USA.
FAU - Spector, Myron
AU  - Spector M
AD  - VA Boston Healthcare System, Brigham and Women's Hospital, Harvard Medical 
      School, Boston, MA, USA.
FAU - Young, Michael
AU  - Young M
AUID- ORCID: 0000-0002-6932-5159
AD  - Schepens Eye Research Institute of Massachusetts Eye and Ear, Harvard Medical 
      School, Boston, MA, USA. Michael_young@meei.harvard.edu.
LA  - eng
GR  - 533330/Rosalinde and Arthur Gilbert Foundation (The Rosalinde and Arthur Gilbert 
      Foundation)/
PT  - Journal Article
DEP - 20211220
PL  - United States
TA  - NPJ Regen Med
JT  - NPJ Regenerative medicine
JID - 101699846
PMC - PMC8688498
COIS- The authors declare no competing interests.
EDAT- 2021/12/22 06:00
MHDA- 2021/12/22 06:01
PMCR- 2021/12/20
CRDT- 2021/12/21 06:18
PHST- 2021/04/16 00:00 [received]
PHST- 2021/10/20 00:00 [accepted]
PHST- 2021/12/21 06:18 [entrez]
PHST- 2021/12/22 06:00 [pubmed]
PHST- 2021/12/22 06:01 [medline]
PHST- 2021/12/20 00:00 [pmc-release]
AID - 10.1038/s41536-021-00195-3 [pii]
AID - 195 [pii]
AID - 10.1038/s41536-021-00195-3 [doi]
PST - epublish
SO  - NPJ Regen Med. 2021 Dec 20;6(1):85. doi: 10.1038/s41536-021-00195-3.