PMID- 34932366
OWN - NLM
STAT- MEDLINE
DCOM- 20220228
LR  - 20230108
IS  - 1535-3907 (Electronic)
IS  - 1535-3893 (Print)
IS  - 1535-3893 (Linking)
VI  - 21
IP  - 1
DP  - 2022 Jan 7
TI  - HLA Allele-Specific Quantitative Profiling of Type 1 Diabetic B Lymphocyte 
      Immunopeptidome.
PG  - 250-264
LID - 10.1021/acs.jproteome.1c00842 [doi]
AB  - Peptide ligands presented by human leukocyte antigen (HLA) molecules on the cell 
      surface represent the immunopeptidome that could be utilized for identification 
      of antigenic peptides for immunotherapy and prevention of autoimmune diseases. 
      Although T-cells are well-known key players in the destruction of pancreatic 
      beta-cells in type 1 diabetes (T1D), increasing evidence points toward a role for 
      B-cells in disease pathogenesis. However, as antigen presenting cells, little is 
      known about the comprehensive immunopeptidome of B cells and their changes in the 
      context of T1D. We performed HLA allele-specific quantitative immunopeptidomics 
      using B lymphocytes derived from T1D patients and healthy controls. Hundreds of 
      HLA-I and HLA-II immunopeptides were identified as differentially regulated in 
      T1D per HLA allele for B cells sharing identical HLA alleles. The results were 
      further validated using additional T1D and healthy B cells with partially 
      overlapped HLA alleles. Differentially expressed immunopeptides were confirmed 
      with targeted proteomics and for reactivity using known T-cell assays in the 
      immune epitope database. Considering samples with identical HLA alleles are 
      difficult to obtain for T1D and other similar HLA-restricted diseases, our work 
      represents a viable approach to better understand HLA allele-specific antigen 
      presentation and may facilitate identification of immunopeptides for therapeutic 
      applications in autoimmune diseases. Data are available via ProteomeXchange with 
      identifier PXD026184.
FAU - Sudhir, Putty-Reddy
AU  - Sudhir PR
AD  - Center for Translational Biomedical Research, University of North Carolina at 
      Greensboro, North Carolina Research Campus, Kannapolis, North Carolina 28081, 
      United States.
FAU - Lin, Tai-Du
AU  - Lin TD
AD  - Center for Translational Biomedical Research, University of North Carolina at 
      Greensboro, North Carolina Research Campus, Kannapolis, North Carolina 28081, 
      United States.
FAU - Zhang, Qibin
AU  - Zhang Q
AUID- ORCID: 0000-0002-6135-8706
AD  - Center for Translational Biomedical Research, University of North Carolina at 
      Greensboro, North Carolina Research Campus, Kannapolis, North Carolina 28081, 
      United States.
AD  - Department of Chemistry & Biochemistry, University of North Carolina at 
      Greensboro, Greensboro, North Carolina 27412, United States.
LA  - eng
GR  - R01 DK114345/DK/NIDDK NIH HHS/United States
GR  - R01 DK116731/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20211221
PL  - United States
TA  - J Proteome Res
JT  - Journal of proteome research
JID - 101128775
RN  - 0 (HLA Antigens)
RN  - 0 (Histocompatibility Antigens Class I)
SB  - IM
MH  - Alleles
MH  - B-Lymphocytes
MH  - *Diabetes Mellitus, Type 1/metabolism
MH  - HLA Antigens
MH  - Histocompatibility Antigens Class I
MH  - Humans
PMC - PMC8742597
MID - NIHMS1768692
OTO - NOTNLM
OT  - B lymphocytes
OT  - HLA allele
OT  - HLA ligandome
OT  - HLA-I
OT  - HLA-II
OT  - immunopeptidome
OT  - type 1 diabetes
EDAT- 2021/12/22 06:00
MHDA- 2022/03/01 06:00
PMCR- 2023/01/07
CRDT- 2021/12/21 17:13
PHST- 2021/12/22 06:00 [pubmed]
PHST- 2022/03/01 06:00 [medline]
PHST- 2021/12/21 17:13 [entrez]
PHST- 2023/01/07 00:00 [pmc-release]
AID - 10.1021/acs.jproteome.1c00842 [doi]
PST - ppublish
SO  - J Proteome Res. 2022 Jan 7;21(1):250-264. doi: 10.1021/acs.jproteome.1c00842. 
      Epub 2021 Dec 21.