PMID- 34936289
OWN - NLM
STAT- MEDLINE
DCOM- 20220316
LR  - 20220531
IS  - 1045-4403 (Print)
IS  - 1045-4403 (Linking)
VI  - 31
IP  - 6
DP  - 2021
TI  - CircRNA circSEPT9 Downregulates miR-10a through Methylation to Promote Cell 
      Proliferation in Laryngeal Squamous Cell Carcinoma.
PG  - 17-22
LID - 10.1615/CritRevEukaryotGeneExpr.2021039092 [doi]
AB  - The oncogenic functions of circRNA circSEPT9 have been characterized in 
      triple-negative breast cancer. We analyzed its role in laryngeal squamous cell 
      carcinoma (LSCC). Quantitative reverse-transcription PCR (RT-qPCR) was used to 
      analyze the expression of circSEPT9 and miR-10a in paired LSCC and nontumor 
      tissues donated by 50 patients with LSCC. Methylation-specific PCR (MSP) was 
      performed to analyze the role of circSEPT9 in miR-10a RNA gene methylation. 
      circSEPT9 or miR-10a were overexpressed in LSCC cells to explore the interaction 
      between them. The regulatory role of circSEPT9 and miR-10a in cell proliferation 
      was studied with cell counting kit-8 (CCK-8) assay. CircSEPT9 was highly 
      expressed in LSCC, whereas miR-10a was expressed at a lower level in LSCC. 
      CircSEPT9 and miR-10a were closely correlated across LSCC tissue samples. In LSCC 
      cells, circSEPT9 overexpression increased the methylation of the miR-10a gene and 
      decreased the expression of miR-10a. CircSEPT9 overexpression increased LSCC cell 
      proliferation, whereas miR-10a overexpression decreased cell proliferation. 
      Co-transfection experiments showed that circSEPT9 overexpression attenuated the 
      effects of miR-10a overexpression on cell proliferation. We conclude that 
      circSEPT9 may increase miR-10a methylation to increase cell proliferation in 
      LSCC.
FAU - Zhu, Minhui
AU  - Zhu M
AD  - Department of Otorhinolaryngology - Head and Neck Surgery, Changhai Hospital, 
      Naval Medical University, Shanghai, People's Republic of China.
FAU - Liu, Chang
AU  - Liu C
AD  - Department of Otolaryngology, LongHua Hospital, Shanghai University of 
      Traditional Chinese Medicine, Shanghai, People's Republic of China.
FAU - Chen, Shicai
AU  - Chen S
AD  - Department of Otorhinolaryngology - Head and Neck Surgery, Changhai Hospital, 
      Naval Medical University, Shanghai, People's Republic of China.
FAU - Zhang, Caiyun
AU  - Zhang C
AD  - Department of Otorhinolaryngology - Head and Neck Surgery, Changhai Hospital, 
      Naval Medical University, Shanghai, People's Republic of China.
FAU - Zhou, Peng
AU  - Zhou P
AD  - Department of Otorhinolaryngology - Head and Neck Surgery, Changhai Hospital, 
      Naval Medical University, Shanghai, People's Republic of China.
FAU - Sun, Jianxiong
AU  - Sun J
AD  - Department of Otorhinolaryngology - Head and Neck Surgery, Changhai Hospital, 
      Naval Medical University, Shanghai, People's Republic of China.
FAU - Zheng, Hongliang
AU  - Zheng H
AD  - Department of Otorhinolaryngology - Head and Neck Surgery, Changhai Hospital, 
      Naval Medical University, Shanghai, 200433, PR. China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Crit Rev Eukaryot Gene Expr
JT  - Critical reviews in eukaryotic gene expression
JID - 9007261
RN  - 0 (MIRN10 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (RNA, Circular)
SB  - IM
MH  - Cell Line, Tumor
MH  - Cell Proliferation/genetics
MH  - Gene Expression Regulation, Neoplastic
MH  - *Head and Neck Neoplasms/genetics
MH  - Humans
MH  - *Laryngeal Neoplasms/genetics/metabolism/pathology
MH  - Methylation
MH  - *MicroRNAs/genetics/metabolism
MH  - RNA, Circular/genetics
MH  - *Squamous Cell Carcinoma of Head and Neck/genetics
EDAT- 2021/12/23 06:00
MHDA- 2022/03/17 06:00
CRDT- 2021/12/22 13:09
PHST- 2021/12/22 13:09 [entrez]
PHST- 2021/12/23 06:00 [pubmed]
PHST- 2022/03/17 06:00 [medline]
AID - 61e879f84b23b688,2a656a64408c286c [pii]
AID - 10.1615/CritRevEukaryotGeneExpr.2021039092 [doi]
PST - ppublish
SO  - Crit Rev Eukaryot Gene Expr. 2021;31(6):17-22. doi: 
      10.1615/CritRevEukaryotGeneExpr.2021039092.