PMID- 34938667
OWN - NLM
STAT- MEDLINE
DCOM- 20220127
LR  - 20220127
IS  - 2235-2988 (Electronic)
IS  - 2235-2988 (Linking)
VI  - 11
DP  - 2021
TI  - Pi-Dan-Jian-Qing Decoction Ameliorates Type 2 Diabetes Mellitus Through 
      Regulating the Gut Microbiota and Serum Metabolism.
PG  - 748872
LID - 10.3389/fcimb.2021.748872 [doi]
LID - 748872
AB  - Pi-Dan-Jian-Qing decoction (PDJQ) can been used in the treatment of type 2 
      diabetes mellitus (T2DM) in clinic. However, the protective mechanisms of PDJQ on 
      T2DM remain unknown. Recent studies have shown that the changes in gut microbiota 
      could affect the host metabolism and contribute to progression of T2DM. In this 
      study, we first investigated the therapeutic effects of PDJQ on T2DM rats. 16S 
      rRNA sequencing and untargeted metabolomics analyses were used to investigate the 
      mechanisms of action of PDJQ in the treatment of T2DM. Our results showed that 
      PDJQ treatment could improve the hyperglycemia, hyperlipidemia, insulin 
      resistance (IR) and pathological changes of liver, pancreas, kidney, and colon in 
      T2DM rats. PDJQ could also decrease the levels of pro-inflammatory cytokines and 
      inhibit the oxidative stress. 16S rRNA sequencing showed that PDJQ could decrease 
      the Firmicutes/Bacteroidetes (F to B) ratio at the phylum level. At the genus 
      level, PDJQ could increase the relative abundances of Lactobacillus, Blautia, 
      Bacteroides, Desulfovibrio and Akkermansia and decrease the relative abundance of 
      Prevotella. Serum untargeted metabolomics analysis showed that PDJQ could 
      regulate tryptophan metabolism, histidine metabolism, tricarboxylic acid (TCA) 
      cycle, phenylalanine, tyrosine and tryptophan biosynthesis and tyrosine 
      metabolism pathways. Correlation analysis indicated that the modulatory effects 
      of PDJQ on the tryptophan metabolism, histidine metabolism and TCA cycle pathways 
      were related to alterations in the abundance of Lactobacillus, Bacteroides and 
      Akkermansia. In conclusion, our study revealed the various ameliorative effects 
      of PDJQ on T2DM, including improving the liver and kidney functions and 
      alleviating the hyperglycemia, hyperlipidemia, IR, pathological changes, 
      oxidative stress and inflammatory response. The mechanisms of PDJQ on T2DM are 
      likely linked to an improvement in the dysbiosis of gut microbiota and modulation 
      of tryptophan metabolism, histamine metabolism, and the TCA cycle.
CI  - Copyright (c) 2021 Xie, Liao, Ai, Gao, Zhao, Qu, Xu, Zhang, Wen, Cui and Wang.
FAU - Xie, Xuehua
AU  - Xie X
AD  - First College of Clinical Medicine, Nanjing University of Traditional Chinese 
      Medicine, Jiangsu, China.
AD  - Department of Endocrinology, Yunnan Provincial Hospital of Chinese Medicine, 
      Yunnan, China.
FAU - Liao, Jiabao
AU  - Liao J
AD  - Department of Emergency, Jiaxing Hospital of Traditional Chinese Medicine, 
      Zhejiang, China.
AD  - Jiaxing Key Laboratory of Diabetic Angiopathy Research, Jiaxing Hospital of 
      Traditional Chinese Medicine, Zhejiang, China.
FAU - Ai, Yuanliang
AU  - Ai Y
AD  - Department of Orthopedics, Kunming Municipal Hospital of Traditional Chinese 
      Medicine, Yunnan, China.
FAU - Gao, Jinmei
AU  - Gao J
AD  - Department of Rehabilitation, Fujian People's Hospital of Traditional Chinese 
      Medicine, Fujian, China.
FAU - Zhao, Jie
AU  - Zhao J
AD  - Department of Endocrinology, Yunnan Provincial Hospital of Chinese Medicine, 
      Yunnan, China.
FAU - Qu, Fei
AU  - Qu F
AD  - Department of Emergency, Jiaxing Hospital of Traditional Chinese Medicine, 
      Zhejiang, China.
FAU - Xu, Chao
AU  - Xu C
AD  - Department of Endocrinology, Yunnan Provincial Hospital of Chinese Medicine, 
      Yunnan, China.
FAU - Zhang, Zhaiyi
AU  - Zhang Z
AD  - College of Integrated Chinese and Western Medicine, Tianjin University of 
      Traditional Chinese Medicine, Tianjin, China.
FAU - Wen, Weibo
AU  - Wen W
AD  - Department of Endocrinology, Yunnan Provincial Hospital of Chinese Medicine, 
      Yunnan, China.
FAU - Cui, Huantian
AU  - Cui H
AD  - Shandong Provincial Key Laboratory of Animal Cell and Developmental Biology, 
      School of Life Sciences, Shandong University, Shandong, China.
FAU - Wang, Hongwu
AU  - Wang H
AD  - College of Traditional Chinese Medicine, Tianjin University of Traditional 
      Chinese Medicine, Tianjin, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20211206
PL  - Switzerland
TA  - Front Cell Infect Microbiol
JT  - Frontiers in cellular and infection microbiology
JID - 101585359
RN  - 0 (RNA, Ribosomal, 16S)
SB  - IM
MH  - Animals
MH  - *Diabetes Mellitus, Type 2/drug therapy
MH  - Dysbiosis
MH  - *Gastrointestinal Microbiome
MH  - *Hyperglycemia
MH  - RNA, Ribosomal, 16S/genetics
MH  - Rats
PMC - PMC8685325
OTO - NOTNLM
OT  - Pi-Dan-Jian-Qing decoction
OT  - TCA cycle
OT  - gut microbiota
OT  - histamine metabolism
OT  - tryptophan metabolism
OT  - type 2 diabetes mellitus
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2021/12/24 06:00
MHDA- 2022/01/28 06:00
PMCR- 2021/01/01
CRDT- 2021/12/23 05:52
PHST- 2021/07/28 00:00 [received]
PHST- 2021/11/03 00:00 [accepted]
PHST- 2021/12/23 05:52 [entrez]
PHST- 2021/12/24 06:00 [pubmed]
PHST- 2022/01/28 06:00 [medline]
PHST- 2021/01/01 00:00 [pmc-release]
AID - 10.3389/fcimb.2021.748872 [doi]
PST - epublish
SO  - Front Cell Infect Microbiol. 2021 Dec 6;11:748872. doi: 
      10.3389/fcimb.2021.748872. eCollection 2021.