PMID- 34939134 OWN - NLM STAT- MEDLINE DCOM- 20220119 LR - 20220119 IS - 1432-0614 (Electronic) IS - 0175-7598 (Linking) VI - 106 IP - 2 DP - 2022 Jan TI - Development and application of a colloidal-gold dual immunochromatography strip for detecting African swine fever virus antibodies. PG - 799-810 LID - 10.1007/s00253-021-11706-z [doi] AB - African swine fever (ASF) is an acute and highly contagious infectious disease caused by the African swine fever virus (ASFV). Currently, there is no vaccine against ASF worldwide, and no effective treatment measures are available. For this reason, developing a simple, rapid, specific, and sensitive serological detection method for ASFV antibodies is crucial for the prevention and control of ASF. In this study, a 1:1 mixture of gold-labeled p30 and p72 probes was used as the gold-labeled antigen. The p30 and p72 proteins and their monoclonal antibodies were coated on a nitrocellulose membrane (NC) as a test (T) line and control (C) line, respectively. A colloidal-gold dual immunochromatography strip (ICS) for ASFV p30 and p72 protein antibodies was established. The results showed that the colloidal-gold dual ICS could specifically detect ASFV antibodies within 5-10 min. There was no cross-reaction after testing healthy pig serum; porcine reproductive and respiratory syndrome virus (PRRSV), foot-and-mouth disease type A virus (FMDV-A), foot-and-mouth disease type O virus (FMDV-O), porcine circovirus type 2 (PCV-2), and classical swine fever virus (CSFV) positive sera. A positive result was obtained only for the positive control P1. The sensitivity of the test strips was 1:256, which was equivalent to that of commercially ELISA kits. Their coincidence rate with the two commercial ASFV ELISA antibodies detection kits was higher than 98%. The test strips were stably stored at 18-25 degrees C and 4 degrees C for 4 and 6 months, respectively. The colloidal-gold dual ICS prepared in this study had high sensitivity and specificity and were characterized by rapid detection, simple operation, and easy interpretation of results. Therefore, they are of great significance to diagnose, prevent, and control African swine fever. KEY POINTS: * We establish an antibody detection that is quick and can monitor an ASF infection. * We observe changes in two protein antibodies to dynamically monitor ASF infection. * We use diversified detection on a single test strip to detect both antibodies. CI - (c) 2021. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature. FAU - Wan, Ying AU - Wan Y AD - State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730046, China. FAU - Shi, Zhengwang AU - Shi Z AD - State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730046, China. FAU - Peng, Gaochaung AU - Peng G AD - State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730046, China. FAU - Wang, Lijuan AU - Wang L AD - State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730046, China. FAU - Luo, Juncong AU - Luo J AD - State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730046, China. FAU - Ru, Yi AU - Ru Y AD - State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730046, China. FAU - Zhou, Gaijing AU - Zhou G AD - State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730046, China. FAU - Ma, Yuan AU - Ma Y AD - State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730046, China. FAU - Song, Rui AU - Song R AD - State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730046, China. FAU - Yang, Bo AU - Yang B AD - State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730046, China. FAU - Cao, Liyan AU - Cao L AD - State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730046, China. FAU - Tian, Hong AU - Tian H AUID- ORCID: 0000-0002-1764-4916 AD - State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730046, China. xibeitian0931@163.com. FAU - Zheng, Haixue AU - Zheng H AD - State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730046, China. haixuezheng@163.com. LA - eng GR - 2018YFC0840400/lanzhou veterinary research institute, chinese academy of agricultural sciences/ PT - Journal Article DEP - 20211223 PL - Germany TA - Appl Microbiol Biotechnol JT - Applied microbiology and biotechnology JID - 8406612 RN - 0 (Gold Colloid) SB - IM MH - *African Swine Fever/diagnosis MH - *African Swine Fever Virus MH - Animals MH - Chromatography, Affinity MH - Enzyme-Linked Immunosorbent Assay MH - Gold Colloid MH - Swine OTO - NOTNLM OT - African swine fever virus OT - Colloidal-gold dual immunochromatography strip OT - Rapid antibodies detection OT - p30 and p72 proteins EDAT- 2021/12/24 06:00 MHDA- 2022/01/20 06:00 CRDT- 2021/12/23 05:59 PHST- 2021/07/12 00:00 [received] PHST- 2021/11/20 00:00 [accepted] PHST- 2021/11/08 00:00 [revised] PHST- 2021/12/24 06:00 [pubmed] PHST- 2022/01/20 06:00 [medline] PHST- 2021/12/23 05:59 [entrez] AID - 10.1007/s00253-021-11706-z [pii] AID - 10.1007/s00253-021-11706-z [doi] PST - ppublish SO - Appl Microbiol Biotechnol. 2022 Jan;106(2):799-810. doi: 10.1007/s00253-021-11706-z. Epub 2021 Dec 23.