PMID- 34940120
OWN - NLM
STAT- MEDLINE
DCOM- 20220318
LR  - 20220716
IS  - 1467-3045 (Electronic)
IS  - 1467-3037 (Print)
IS  - 1467-3037 (Linking)
VI  - 43
IP  - 3
DP  - 2021 Nov 26
TI  - Matrix Metalloproteinases in Human Decidualized Endometrial Stromal Cells.
PG  - 2111-2123
LID - 10.3390/cimb43030146 [doi]
AB  - Cyclic changes, such as growth, decidualization, shedding, and regeneration, in 
      the human endometrium are regulated by the reciprocal action of female hormones, 
      such as estradiol (E(2)), and progesterone (P(4)). Matrix metalloproteases (MMPs) 
      and tissue inhibitors of MMPs (TIMPs) control the invasion of extravillous 
      trophoblast cells after implantation. Several MMPs and TIMPs function in the 
      decidua and endometrial stromal cells (ESCs). Here, we aimed to systematically 
      investigate the changes in MMPs and TIMPs associated with ESC decidualization. We 
      evaluated the expression of 23 MMPs, four TIMPs, and four anti-sense non-coding 
      RNAs from MMP loci. Primary ESC cultures treated with E(2) + medroxyprogesterone 
      acetate (MPA), a potent P(4) receptor agonist, showed significant down-regulation 
      of MMP3, MMP10, MMP11, MMP12, MMP20, and MMP27 in decidualized ESCs, as assessed 
      by quantitative reverse transcription PCR. Further, MMP15 and MMP19 were 
      significantly upregulated in decidualized ESCs. siRNA-mediated silencing of Heart 
      and Neural Crest Derivatives Expressed 2 (HAND2), a master transcriptional 
      regulator in ESC decidualization, significantly increased MMP15 expression in 
      untreated human ESCs. These results collectively indicate the importance of MMP15 
      and MMP19 in ESC decidualization and highlight the role of HAND2 in repressing 
      MMP15 transcription, thereby regulating decidualization.
FAU - Hisamatsu, Yoji
AU  - Hisamatsu Y
AD  - Department of Obstetrics and Gynecology, Kansai Medical University, Osaka 
      573-1010, Japan.
FAU - Murata, Hiromi
AU  - Murata H
AUID- ORCID: 0000-0001-9609-0976
AD  - Department of Obstetrics and Gynecology, Kansai Medical University, Osaka 
      573-1010, Japan.
FAU - Tsubokura, Hiroaki
AU  - Tsubokura H
AD  - Department of Obstetrics and Gynecology, Kansai Medical University, Osaka 
      573-1010, Japan.
FAU - Hashimoto, Yoshiko
AU  - Hashimoto Y
AD  - Department of Obstetrics and Gynecology, Kansai Medical University, Osaka 
      573-1010, Japan.
FAU - Kitada, Masaaki
AU  - Kitada M
AD  - Department of Anatomy, Kansai Medical University, Osaka 573-1010, Japan.
FAU - Tanaka, Susumu
AU  - Tanaka S
AUID- ORCID: 0000-0003-4804-7839
AD  - Department of Anatomy, Kansai Medical University, Osaka 573-1010, Japan.
FAU - Okada, Hidetaka
AU  - Okada H
AUID- ORCID: 0000-0002-7932-6999
AD  - Department of Obstetrics and Gynecology, Kansai Medical University, Osaka 
      573-1010, Japan.
LA  - eng
GR  - 21K09480/Japan Society for the Promotion of Science/
GR  - 21K09529/Japan Society for the Promotion of Science/
GR  - 19K06891/Japan Society for the Promotion of Science/
GR  - 2018/Takeda Science Foundation/
PT  - Journal Article
DEP - 20211126
PL  - Switzerland
TA  - Curr Issues Mol Biol
JT  - Current issues in molecular biology
JID - 100931761
RN  - 0 (Biomarkers)
RN  - 0 (Steroids)
RN  - 0 (Tissue Inhibitor of Metalloproteinases)
RN  - EC 3.4.24.- (Matrix Metalloproteinases)
MH  - Adult
MH  - Biomarkers
MH  - Cells, Cultured
MH  - Decidua/*cytology/*metabolism
MH  - Endometrium/*cytology/*metabolism
MH  - Female
MH  - Gene Expression Regulation/drug effects
MH  - Humans
MH  - Matrix Metalloproteinases/genetics/*metabolism
MH  - Middle Aged
MH  - Steroids/metabolism/pharmacology
MH  - Stromal Cells/drug effects/*metabolism
MH  - Tissue Inhibitor of Metalloproteinases/metabolism
MH  - Young Adult
PMC - PMC8929033
OTO - NOTNLM
OT  - HAND2: heart and neural crest derivatives expressed 2
OT  - MMP: matrix metalloprotease
OT  - SNAI1: snail family transcriptional repressor 1
OT  - TIMP: tissue inhibitor of metalloproteinase
COIS- The authors declare no conflict of interest in connection with this article.
EDAT- 2021/12/24 06:00
MHDA- 2022/03/19 06:00
PMCR- 2021/11/26
CRDT- 2021/12/23 12:42
PHST- 2021/11/10 00:00 [received]
PHST- 2021/11/25 00:00 [accepted]
PHST- 2021/12/23 12:42 [entrez]
PHST- 2021/12/24 06:00 [pubmed]
PHST- 2022/03/19 06:00 [medline]
PHST- 2021/11/26 00:00 [pmc-release]
AID - cimb43030146 [pii]
AID - cimb-43-00146 [pii]
AID - 10.3390/cimb43030146 [doi]
PST - epublish
SO  - Curr Issues Mol Biol. 2021 Nov 26;43(3):2111-2123. doi: 10.3390/cimb43030146.