PMID- 34943761
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20211229
IS  - 2079-6382 (Print)
IS  - 2079-6382 (Electronic)
IS  - 2079-6382 (Linking)
VI  - 10
IP  - 12
DP  - 2021 Dec 17
TI  - Carbapenemase Producing Klebsiella pneumoniae (KPC): What Is the Best MALDI-TOF 
      MS Detection Method.
LID - 10.3390/antibiotics10121549 [doi]
LID - 1549
AB  - Klebsiella pneumoniae carbapenemase (KPC)-producing bacteria is a group of highly 
      dangerous antibiotic resistant Gram-negative Enterobacteriaceae. They cause 
      infections associated with significant morbidity and mortality. Therefore, the 
      rapid detection of KPC-producing bacteria plays a key role in clinical 
      microbiology. Matrix assisted laser desorption/ionization time-of- flight 
      (MALDI-TOF) is a rapidly evolving technology that finds application in various 
      clinical, scientific, and industrial disciplines. In the present study, we 
      demonstrated three different procedures of carbapenemase-producing K. pneumoniae 
      (KPC) detection. The most basic model of MALDI-TOF instrument MS Microflex LT was 
      used, operating in the linear ion-positive mode, commonly used in modern clinical 
      laboratories. The first procedure was based on indirect monitoring of 
      carbapenemase production with direct detection of hydrolyzed carbapenem 
      antibiotic degradation products in the mass spectrum. The second procedure was 
      based on direct detection of bla(KPC) accompanying peak with an 11,109 Da in the 
      mass spectrum of carbapenemase-producing K. pneumoniae (KPC), which represents 
      the cleaved protein (pKpQIL_p019) expressed by pKpQIL plasmid. In addition, 
      several unique peaks were detected in the carbapenemase-producing K. pneumoniae 
      (KPC) mass spectrum. The third procedure was the identification of 
      carbapenemase-producing K. pneumoniae (KPC) based on the protein fingerprint 
      using local database created from the whole mass spectra. By comparing detection 
      procedures, we determined that the third procedure was very fast and relatively 
      easy. However, it requires previous verification of carbapenemase-producing K. 
      pneumoniae (KPC) using other methods as genetic bla(KPC) identification, 
      detection of carbapenem degradation products, and accompanying peak with 11,109 
      Da, which represents cleaved pKpQIL_p019 protein expressed by pKpQIL plasmid. 
      Detection of carbapenemase-producing K. pneumoniae using MALDI-TOF provides fast 
      and accurate results that may help to reduce morbidity and mortality in hospital 
      setting when applied in diagnostic situations.
FAU - Hleba, Lukas
AU  - Hleba L
AUID- ORCID: 0000-0001-8244-6548
AD  - Institute of Biotechnology, Faculty of Biotechnology and Food Sciences, Slovak 
      University of Agriculture, Trieda Andreja Hlinku 2, 949 76 Nitra, Slovakia.
FAU - Hlebova, Miroslava
AU  - Hlebova M
AUID- ORCID: 0000-0002-1720-9981
AD  - Department of Biology, Faculty of Natural Sciences, University of Ss. Cyril and 
      Methodius in Trnava, Nam. Jana Herdu 577/2, 917 01 Trnava, Slovakia.
FAU - Kovacik, Anton
AU  - Kovacik A
AUID- ORCID: 0000-0003-4636-1795
AD  - Institute of Applied Biology, Faculty of Biotechnology and Food Sciences, Slovak 
      University of Agriculture, Trieda Andreja Hlinku 2, 949 76 Nitra, Slovakia.
FAU - Cubon, Juraj
AU  - Cubon J
AD  - Institute of Food Sciences, Faculty of Biotechnology and Food Sciences, Slovak 
      University of Agriculture, Trieda Andreja Hlinku 2, 949 76 Nitra, Slovakia.
FAU - Medo, Juraj
AU  - Medo J
AD  - Institute of Biotechnology, Faculty of Biotechnology and Food Sciences, Slovak 
      University of Agriculture, Trieda Andreja Hlinku 2, 949 76 Nitra, Slovakia.
LA  - eng
GR  - VEGA 1/0661/19/Ministry of Education, Science, Research and Sport of the Slovak 
      Republic/
PT  - Journal Article
DEP - 20211217
PL  - Switzerland
TA  - Antibiotics (Basel)
JT  - Antibiotics (Basel, Switzerland)
JID - 101637404
PMC - PMC8698427
OTO - NOTNLM
OT  - KPC
OT  - Klebsiella pneumoniae
OT  - MALDI-TOF MS
OT  - detection
COIS- The authors declare no conflict of interest.
EDAT- 2021/12/25 06:00
MHDA- 2021/12/25 06:01
PMCR- 2021/12/17
CRDT- 2021/12/24 01:03
PHST- 2021/11/15 00:00 [received]
PHST- 2021/12/14 00:00 [revised]
PHST- 2021/12/16 00:00 [accepted]
PHST- 2021/12/24 01:03 [entrez]
PHST- 2021/12/25 06:00 [pubmed]
PHST- 2021/12/25 06:01 [medline]
PHST- 2021/12/17 00:00 [pmc-release]
AID - antibiotics10121549 [pii]
AID - antibiotics-10-01549 [pii]
AID - 10.3390/antibiotics10121549 [doi]
PST - epublish
SO  - Antibiotics (Basel). 2021 Dec 17;10(12):1549. doi: 10.3390/antibiotics10121549.