PMID- 34943870
OWN - NLM
STAT- MEDLINE
DCOM- 20220119
LR  - 20240404
IS  - 2073-4409 (Electronic)
IS  - 2073-4409 (Linking)
VI  - 10
IP  - 12
DP  - 2021 Nov 30
TI  - Dendritic Cell Migration Is Tuned by Mechanical Stiffness of the Confining Space.
LID - 10.3390/cells10123362 [doi]
LID - 3362
AB  - The coordination of cell migration of immune cells is a critical aspect of the 
      immune response to pathogens. Dendritic cells (DCs), the sentinels of the immune 
      system, are exposed to complex tissue microenvironments with a wide range of 
      stiffnesses. Recent studies have revealed the importance of mechanical cues in 
      immune cell trafficking in confined 3D environments. However, the mechanism by 
      which stiffness modulates the intrinsic motility of immature DCs remains poorly 
      understood. Here, immature DCs were found to navigate confined spaces in a rapid 
      and persistent manner, surveying a wide range when covered with compliant gels 
      mimicking soft tissues. However, the speed and persistence time of random 
      motility were both decreased by confinement in gels with higher stiffness, 
      mimicking skin or diseased, fibrotic tissue. The impact of stiffness of 
      surrounding tissue is crucial because most in vitro studies to date have been 
      based on cellular locomotion when confined by microfabricated 
      polydimethylsiloxane structures. Our study provides evidence for a role for 
      environmental mechanical stiffness in the surveillance strategy of immature DCs 
      in tissues.
FAU - Choi, Yongjun
AU  - Choi Y
AUID- ORCID: 0000-0002-3814-3643
AD  - Department of Biomedical Engineering, Ulsan National Institute of Science and 
      Technology (UNIST), Ulsan 44919, Korea.
AD  - Center for Soft and Living Matter, Institute for Basic Science (IBS), Ulsan 
      44919, Korea.
FAU - Kwon, Jae-Eun
AU  - Kwon JE
AD  - Department of Material Science and Engineering, Ulsan National Institute of 
      Science and Technology (UNIST), Ulsan 44919, Korea.
FAU - Cho, Yoon-Kyoung
AU  - Cho YK
AUID- ORCID: 0000-0001-6423-1834
AD  - Department of Biomedical Engineering, Ulsan National Institute of Science and 
      Technology (UNIST), Ulsan 44919, Korea.
AD  - Center for Soft and Living Matter, Institute for Basic Science (IBS), Ulsan 
      44919, Korea.
LA  - eng
GR  - IBS-R020-D1/Institute for Basic Science/
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20211130
PL  - Switzerland
TA  - Cells
JT  - Cells
JID - 101600052
SB  - IM
MH  - Animals
MH  - Biomechanical Phenomena
MH  - *Cell Movement
MH  - Cell Shape
MH  - Dendritic Cells/*cytology
MH  - Diffusion
MH  - Humans
MH  - *Stress, Mechanical
PMC - PMC8699733
OTO - NOTNLM
OT  - cell migration
OT  - confinement
OT  - dendritic cell
OT  - elastic modulus
OT  - hydrogel
OT  - stiffness
COIS- The authors declare no conflict of interest.
EDAT- 2021/12/25 06:00
MHDA- 2022/01/20 06:00
PMCR- 2021/11/30
CRDT- 2021/12/24 01:03
PHST- 2021/10/28 00:00 [received]
PHST- 2021/11/20 00:00 [revised]
PHST- 2021/11/22 00:00 [accepted]
PHST- 2021/12/24 01:03 [entrez]
PHST- 2021/12/25 06:00 [pubmed]
PHST- 2022/01/20 06:00 [medline]
PHST- 2021/11/30 00:00 [pmc-release]
AID - cells10123362 [pii]
AID - cells-10-03362 [pii]
AID - 10.3390/cells10123362 [doi]
PST - epublish
SO  - Cells. 2021 Nov 30;10(12):3362. doi: 10.3390/cells10123362.