PMID- 34944048
OWN - NLM
STAT- MEDLINE
DCOM- 20220107
LR  - 20220107
IS  - 2073-4409 (Electronic)
IS  - 2073-4409 (Linking)
VI  - 10
IP  - 12
DP  - 2021 Dec 15
TI  - Clever Experimental Designs: Shortcuts for Better iPSC Differentiation.
LID - 10.3390/cells10123540 [doi]
LID - 3540
AB  - For practical use of pluripotent stem cells (PSCs) for disease modelling, drug 
      screening, and regenerative medicine, the cell differentiation process needs to 
      be properly refined to generate end products with consistent and high quality. To 
      construct and optimize a robust cell-induction process, a myriad of cell culture 
      conditions should be considered. In contrast to inefficient brute-force 
      screening, statistical design of experiments (DOE) approaches, such as factorial 
      design, orthogonal array design, response surface methodology (RSM), definitive 
      screening design (DSD), and mixture design, enable efficient and strategic 
      screening of conditions in smaller experimental runs through multifactorial 
      screening and/or quantitative modeling. Although DOE has become routinely 
      utilized in the bioengineering and pharmaceutical fields, the imminent need of 
      more detailed cell-lineage specification, complex organoid construction, and a 
      stable supply of qualified cell-derived material requires expedition of DOE 
      utilization in stem cell bioprocessing. This review summarizes DOE-based cell 
      culture optimizations of PSCs, mesenchymal stem cells (MSCs), hematopoietic stem 
      cells (HSCs), and Chinese hamster ovary (CHO) cells, which guide effective 
      research and development of PSC-derived materials for academic and industrial 
      applications.
FAU - Yasui, Ryota
AU  - Yasui R
AD  - Department of Regenerative Medicine, Yokohama City University Graduate School of 
      Medicine, Yokohama 236-0004, Japan.
AD  - Fundamental Research Laboratory, Eiken Chemical Co., Ltd., Tochigi 329-0114, 
      Japan.
FAU - Sekine, Keisuke
AU  - Sekine K
AUID- ORCID: 0000-0001-5133-0876
AD  - Department of Regenerative Medicine, Yokohama City University Graduate School of 
      Medicine, Yokohama 236-0004, Japan.
AD  - Laboratory of Cancer Cell Systems, National Cancer Center Research Institute, 
      Tokyo 104-0045, Japan.
FAU - Taniguchi, Hideki
AU  - Taniguchi H
AD  - Department of Regenerative Medicine, Yokohama City University Graduate School of 
      Medicine, Yokohama 236-0004, Japan.
AD  - Division of Regenerative Medicine, Center for Stem Cell Biology and Regenerative 
      Medicine, The Institute of Medical Science, The University of Tokyo, Tokyo 
      108-8639, Japan.
LA  - eng
GR  - None/Japan Agency for Medical Research and Development/
GR  - 18K19589/Ministry of Education, Culture, Sports, Science and Technology of Japan/
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Review
DEP - 20211215
PL  - Switzerland
TA  - Cells
JT  - Cells
JID - 101600052
SB  - IM
MH  - Animals
MH  - *Cell Differentiation
MH  - Cell Proliferation
MH  - Decision Trees
MH  - Humans
MH  - Induced Pluripotent Stem Cells/*cytology
MH  - Research Design
PMC - PMC8700474
OTO - NOTNLM
OT  - Chinese hamster ovary (CHO) cell
OT  - cell differentiation
OT  - design of experiments (DOE)
OT  - embryonic stem cell (ESC)
OT  - hematopoietic stem cell (HSC)
OT  - induced pluripotent stem cell (iPSC)
OT  - mesenchymal stem cell (MSC)
COIS- R.Y. is employee of Eiken Chemical Co., Ltd. Authors declare no conflict of 
      interest.
EDAT- 2021/12/25 06:00
MHDA- 2022/01/08 06:00
PMCR- 2021/12/15
CRDT- 2021/12/24 01:04
PHST- 2021/11/16 00:00 [received]
PHST- 2021/12/10 00:00 [revised]
PHST- 2021/12/11 00:00 [accepted]
PHST- 2021/12/24 01:04 [entrez]
PHST- 2021/12/25 06:00 [pubmed]
PHST- 2022/01/08 06:00 [medline]
PHST- 2021/12/15 00:00 [pmc-release]
AID - cells10123540 [pii]
AID - cells-10-03540 [pii]
AID - 10.3390/cells10123540 [doi]
PST - epublish
SO  - Cells. 2021 Dec 15;10(12):3540. doi: 10.3390/cells10123540.