PMID- 34945663
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20231108
IS  - 2304-8158 (Print)
IS  - 2304-8158 (Electronic)
IS  - 2304-8158 (Linking)
VI  - 10
IP  - 12
DP  - 2021 Dec 15
TI  - Potential of Flow Cytometric Approaches for Rapid Microbial Detection and 
      Characterization in the Food Industry-A Review.
LID - 10.3390/foods10123112 [doi]
LID - 3112
AB  - As microbial contamination is persistent within the food and bioindustries and 
      foodborne infections are still a significant cause of death, the detection, 
      monitoring, and characterization of pathogens and spoilage microorganisms are of 
      great importance. However, the current methods do not meet all relevant criteria. 
      They either show (i) inadequate sensitivity, rapidity, and effectiveness; (ii) a 
      high workload and time requirement; or (iii) difficulties in differentiating 
      between viable and non-viable cells. Flow cytometry (FCM) represents an approach 
      to overcome such limitations. Thus, this comprehensive literature review focuses 
      on the potential of FCM and fluorescence in situ hybridization (FISH) for food 
      and bioindustry applications. First, the principles of FCM and FISH and basic 
      staining methods are discussed, and critical areas for microbial contamination, 
      including abiotic and biotic surfaces, water, and air, are characterized. 
      State-of-the-art non-specific FCM and specific FISH approaches are described, and 
      their limitations are highlighted. One such limitation is the use of toxic and 
      mutagenic fluorochromes and probes. Alternative staining and hybridization 
      approaches are presented, along with other strategies to overcome the current 
      challenges. Further research needs are outlined in order to make FCM and FISH 
      even more suitable monitoring and detection tools for food quality and safety and 
      environmental and clinical approaches.
FAU - Zand, Elena
AU  - Zand E
AUID- ORCID: 0000-0001-6132-6467
AD  - Department of Food Science and Technology, Institute of Food Technology, 
      University of Natural Resources and Life Sciences Vienna (BOKU), 1190 Vienna, 
      Austria.
FAU - Froehling, Antje
AU  - Froehling A
AD  - Leibniz Institute for Agricultural Engineering and Bioeconomy, Quality and Safety 
      of Food and Feed, 14469 Potsdam, Germany.
FAU - Schoenher, Christoph
AU  - Schoenher C
AD  - Institute of Sanitary Engineering and Water Pollution Control, University of 
      Natural Resources and Life Sciences, 1190 Vienna, Austria.
FAU - Zunabovic-Pichler, Marija
AU  - Zunabovic-Pichler M
AD  - Institute of Sanitary Engineering and Water Pollution Control, University of 
      Natural Resources and Life Sciences, 1190 Vienna, Austria.
FAU - Schlueter, Oliver
AU  - Schlueter O
AUID- ORCID: 0000-0002-6806-9886
AD  - Leibniz Institute for Agricultural Engineering and Bioeconomy, Quality and Safety 
      of Food and Feed, 14469 Potsdam, Germany.
FAU - Jaeger, Henry
AU  - Jaeger H
AD  - Department of Food Science and Technology, Institute of Food Technology, 
      University of Natural Resources and Life Sciences Vienna (BOKU), 1190 Vienna, 
      Austria.
LA  - eng
GR  - 866346/Austrian Research Promotion Agency/
PT  - Journal Article
PT  - Review
DEP - 20211215
PL  - Switzerland
TA  - Foods
JT  - Foods (Basel, Switzerland)
JID - 101670569
PMC - PMC8701031
OTO - NOTNLM
OT  - flow cytometry
OT  - fluorescence in situ hybridization
OT  - food safety
OT  - inline monitoring
OT  - microbial contamination
COIS- The authors declare no conflict of interest.
EDAT- 2021/12/25 06:00
MHDA- 2021/12/25 06:01
PMCR- 2021/12/15
CRDT- 2021/12/24 01:09
PHST- 2021/10/07 00:00 [received]
PHST- 2021/11/14 00:00 [revised]
PHST- 2021/11/16 00:00 [accepted]
PHST- 2021/12/24 01:09 [entrez]
PHST- 2021/12/25 06:00 [pubmed]
PHST- 2021/12/25 06:01 [medline]
PHST- 2021/12/15 00:00 [pmc-release]
AID - foods10123112 [pii]
AID - foods-10-03112 [pii]
AID - 10.3390/foods10123112 [doi]
PST - epublish
SO  - Foods. 2021 Dec 15;10(12):3112. doi: 10.3390/foods10123112.