PMID- 34946091
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20211229
IS  - 2076-2607 (Print)
IS  - 2076-2607 (Electronic)
IS  - 2076-2607 (Linking)
VI  - 9
IP  - 12
DP  - 2021 Dec 1
TI  - A Cell Culture System to Investigate Marek's Disease Virus Integration into Host 
      Chromosomes.
LID - 10.3390/microorganisms9122489 [doi]
LID - 2489
AB  - Marek's disease virus (MDV) is a highly oncogenic alphaherpesvirus that causes a 
      devastating neoplastic disease in chickens. MDV has been shown to integrate its 
      genome into the telomeres of latently infected and tumor cells, which is crucial 
      for efficient tumor formation. Telomeric repeat arrays present at the ends of the 
      MDV genome facilitate this integration into host telomeres; however, the 
      integration mechanism remains poorly understood. Until now, MDV integration could 
      only be investigated qualitatively upon infection of chickens. To shed further 
      light on the integration mechanism, we established a quantitative integration 
      assay using chicken T cell lines, the target cells for MDV latency and 
      transformation. We optimized the infection conditions and assessed the 
      establishment of latency in these T cells. The MDV genome was efficiently 
      maintained over time, and integration was confirmed in these cells by 
      fluorescence in situ hybridization (FISH). To assess the role of the two distinct 
      viral telomeric repeat arrays in the integration process, we tested various 
      knockout mutants in our in vitro integration assay. Efficient genome maintenance 
      and integration was thereby dependent on the presence of the telomeric repeat 
      arrays in the virus genome. Taken together, we developed and validated a novel in 
      vitro integration assay that will shed light on the integration mechanism of this 
      highly oncogenic virus into host telomeres.
FAU - You, Yu
AU  - You Y
AD  - Institute of Virology, Freie Universitat Berlin, 14163 Berlin, Germany.
FAU - Vychodil, Tereza
AU  - Vychodil T
AD  - Institute of Virology, Freie Universitat Berlin, 14163 Berlin, Germany.
FAU - Aimola, Giulia
AU  - Aimola G
AD  - Institute of Virology, Freie Universitat Berlin, 14163 Berlin, Germany.
FAU - Previdelli, Renato L
AU  - Previdelli RL
AD  - Institute of Virology, Freie Universitat Berlin, 14163 Berlin, Germany.
AD  - Department of Comparative Biomedical Sciences, Royal Veterinary College, London 
      NW1 0TU, UK.
FAU - Gobel, Thomas W
AU  - Gobel TW
AD  - Institute for Animal Physiology, Department of Veterinary Sciences, Ludwig 
      Maximilian University Munich, 80539 Munich, Germany.
FAU - Bertzbach, Luca D
AU  - Bertzbach LD
AUID- ORCID: 0000-0002-0698-5395
AD  - Institute of Virology, Freie Universitat Berlin, 14163 Berlin, Germany.
AD  - Department of Viral Transformation, Leibniz Institute for Experimental Virology 
      (HPI), 20251 Hamburg, Germany.
FAU - Kaufer, Benedikt B
AU  - Kaufer BB
AUID- ORCID: 0000-0003-1328-2695
AD  - Institute of Virology, Freie Universitat Berlin, 14163 Berlin, Germany.
LA  - eng
GR  - Stg 677673/ERC_/European Research Council/International
GR  - A112662/Volkswagen Foundation/
PT  - Journal Article
DEP - 20211201
PL  - Switzerland
TA  - Microorganisms
JT  - Microorganisms
JID - 101625893
PMC - PMC8706938
OTO - NOTNLM
OT  - MDV
OT  - fluorescence in situ hybridization
OT  - genome maintenance
OT  - herpesvirus
OT  - in vitro assay
OT  - latency
OT  - telomere integration
OT  - telomeric repeats
OT  - viral transformation
COIS- The authors declare no competing interest.
EDAT- 2021/12/25 06:00
MHDA- 2021/12/25 06:01
PMCR- 2021/12/01
CRDT- 2021/12/24 01:10
PHST- 2021/10/18 00:00 [received]
PHST- 2021/11/25 00:00 [revised]
PHST- 2021/11/29 00:00 [accepted]
PHST- 2021/12/24 01:10 [entrez]
PHST- 2021/12/25 06:00 [pubmed]
PHST- 2021/12/25 06:01 [medline]
PHST- 2021/12/01 00:00 [pmc-release]
AID - microorganisms9122489 [pii]
AID - microorganisms-09-02489 [pii]
AID - 10.3390/microorganisms9122489 [doi]
PST - epublish
SO  - Microorganisms. 2021 Dec 1;9(12):2489. doi: 10.3390/microorganisms9122489.