PMID- 34947032
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20211229
IS  - 2309-608X (Electronic)
IS  - 2309-608X (Linking)
VI  - 7
IP  - 12
DP  - 2021 Dec 8
TI  - MicroRNA-6498-5p Inhibits Nosema bombycis Proliferation by Downregulating BmPLPP2 
      in Bombyx mori.
LID - 10.3390/jof7121051 [doi]
LID - 1051
AB  - As microRNAs (miRNAs) are important expression regulators of coding RNA, it is 
      important to characterize their role in the interaction between hosts and 
      pathogens. To obtain a comprehensive understanding of the miRNA alternation in 
      Bombyx mori (B. mori) infected with Nosema bombycis (N. bombycis), RNA sequencing 
      and stem-loop qPCR were conducted to screen and identify the significantly 
      differentially expressed miRNAs (DEmiRNAs). A total of 17 such miRNAs were 
      identified in response to N. bombycis infection, among which miR6498-5p 
      efficiently inhibited the proliferation of N. bombycis in BmE-SWU1 (BmE) cells by 
      downregulating pyridoxal phosphate phosphatase 2 (BmPLPP2). In addition, a 
      fluorescence in situ hybridization (FISH) assay showed that miR6498-5p was 
      located in the cytoplasm of BmE cells, while it was not found in the schizonts of 
      N. bombycis. Further investigation of the effect of BmPLPP2 on the proliferation 
      of schizonts found that the positive factor BmPLPP2 could facilitate N. bombycis 
      completing its life cycle in cells by overexpression and RNAi of BmPLPP2. Our 
      findings offer multiple new insights into the role of miRNAs in the interaction 
      between hosts and microsporidia.
FAU - Hu, Congwu
AU  - Hu C
AUID- ORCID: 0000-0001-6068-4748
AD  - State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 
      400716, China.
FAU - Dong, Zhanqi
AU  - Dong Z
AUID- ORCID: 0000-0002-0607-4241
AD  - State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 
      400716, China.
AD  - Key Laboratory of Sericultural Biology and Genetic Breeding, Ministry of 
      Agriculture and Rural Affairs, Southwest University, Chongqing 400716, China.
FAU - Deng, Boyuan
AU  - Deng B
AD  - State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 
      400716, China.
FAU - Wu, Qin
AU  - Wu Q
AD  - State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 
      400716, China.
FAU - Chen, Peng
AU  - Chen P
AD  - State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 
      400716, China.
AD  - Key Laboratory of Sericultural Biology and Genetic Breeding, Ministry of 
      Agriculture and Rural Affairs, Southwest University, Chongqing 400716, China.
FAU - Lu, Cheng
AU  - Lu C
AD  - State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 
      400716, China.
AD  - Key Laboratory of Sericultural Biology and Genetic Breeding, Ministry of 
      Agriculture and Rural Affairs, Southwest University, Chongqing 400716, China.
FAU - Pan, Minhui
AU  - Pan M
AD  - State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 
      400716, China.
AD  - Key Laboratory of Sericultural Biology and Genetic Breeding, Ministry of 
      Agriculture and Rural Affairs, Southwest University, Chongqing 400716, China.
LA  - eng
GR  - 31872427/National Natural Science Foundation of China/
GR  - 31902214/National Natural Science Foundation of China/
GR  - XDJK2020C010/Fundamental Research Funds for the Central Universities/
GR  - SWU120008/Fundamental Research Funds for the Central Universities/
GR  - cstc2020jscx-msxmX0045/Natural Science Foundation of Chongqing/
GR  - cstc2019jcyj-msxmX0096/Natural Science Foundation of Chongqing/
GR  - CARS-18/the China Agriculture Research System/
PT  - Journal Article
DEP - 20211208
PL  - Switzerland
TA  - J Fungi (Basel)
JT  - Journal of fungi (Basel, Switzerland)
JID - 101671827
PMC - PMC8707756
OTO - NOTNLM
OT  - B. mori
OT  - BmPLPP2
OT  - N. bombycis
OT  - anti-microsporidian
OT  - miR6498-5p
OT  - microRNA
COIS- The authors no potential conflict of interest.
EDAT- 2021/12/25 06:00
MHDA- 2021/12/25 06:01
PMCR- 2021/12/08
CRDT- 2021/12/24 01:13
PHST- 2021/11/14 00:00 [received]
PHST- 2021/12/02 00:00 [revised]
PHST- 2021/12/06 00:00 [accepted]
PHST- 2021/12/24 01:13 [entrez]
PHST- 2021/12/25 06:00 [pubmed]
PHST- 2021/12/25 06:01 [medline]
PHST- 2021/12/08 00:00 [pmc-release]
AID - jof7121051 [pii]
AID - jof-07-01051 [pii]
AID - 10.3390/jof7121051 [doi]
PST - epublish
SO  - J Fungi (Basel). 2021 Dec 8;7(12):1051. doi: 10.3390/jof7121051.