PMID- 34947838
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20211229
IS  - 2075-1729 (Print)
IS  - 2075-1729 (Electronic)
IS  - 2075-1729 (Linking)
VI  - 11
IP  - 12
DP  - 2021 Nov 27
TI  - Coupling/Uncoupling Reversibility in Isolated Mitochondria from Saccharomyces 
      cerevisiae.
LID - 10.3390/life11121307 [doi]
LID - 1307
AB  - The yeast Saccharomyces cerevisiae uses fermentation as the preferred pathway to 
      obtain ATP and requires the respiratory chain to re-oxidize the NADH needed for 
      activity of Glyceraldehyde-3-phosphate. This process is favored by uncoupling of 
      oxidative phosphorylation (OxPhos), which is at least partially controlled by the 
      mitochondrial unspecific pore ((Sc)MUC). When mitochondrial ATP synthesis is 
      needed as in the diauxic phase or during mating, a large rise in Ca(2+) 
      concentration ([Ca(2+)]) closes (Sc)MUC, coupling OxPhos. In addition, (Sc)MUC 
      opening/closing is mediated by the ATP/ADP ratio, which indicates cellular energy 
      needs. Here, opening and closing of (Sc)MUC was evaluated in isolated 
      mitochondria from S. cerevisiae at different incubation times and in the presence 
      of different ATP/ADP ratios or varying [Ca(2+)]. Measurements of the rate of O(2) 
      consumption, mitochondrial swelling, transmembrane potential and ROS generation 
      were conducted. It was observed that (Sc)MUC opening was reversible, a high 
      ATP/ADP ratio promoted opening and [Ca(2+)] closed (Sc)MUC even after several 
      minutes of incubation in the open state. In the absence of ATP synthesis, closure 
      of (Sc)MUC resulted in an increase in ROS.
FAU - Morales-Garcia, Lilia
AU  - Morales-Garcia L
AUID- ORCID: 0000-0001-7184-417X
AD  - Department of Genetics and Molecular Biology, Instituto de Fisiologia Celular, 
      UNAM, Mexico City 04510, Mexico.
AD  - Department of Biochemistry, Medicine School, UNAM, Mexico City 04510, Mexico.
FAU - Ricardez-Garcia, Carolina
AU  - Ricardez-Garcia C
AD  - Department of Genetics and Molecular Biology, Instituto de Fisiologia Celular, 
      UNAM, Mexico City 04510, Mexico.
FAU - Castaneda-Tamez, Paulina
AU  - Castaneda-Tamez P
AD  - Department of Genetics and Molecular Biology, Instituto de Fisiologia Celular, 
      UNAM, Mexico City 04510, Mexico.
FAU - Chiquete-Felix, Natalia
AU  - Chiquete-Felix N
AD  - Department of Genetics and Molecular Biology, Instituto de Fisiologia Celular, 
      UNAM, Mexico City 04510, Mexico.
FAU - Uribe-Carvajal, Salvador
AU  - Uribe-Carvajal S
AUID- ORCID: 0000-0002-7028-8121
AD  - Department of Genetics and Molecular Biology, Instituto de Fisiologia Celular, 
      UNAM, Mexico City 04510, Mexico.
AD  - Department of Biochemistry, Medicine School, UNAM, Mexico City 04510, Mexico.
LA  - eng
GR  - 208821/DGAPA/PAPIIT Natl University of Mexico/
PT  - Journal Article
DEP - 20211127
PL  - Switzerland
TA  - Life (Basel)
JT  - Life (Basel, Switzerland)
JID - 101580444
PMC - PMC8707985
OTO - NOTNLM
OT  - Ca2+
OT  - ScMUC
OT  - mitochondrial permeability transition reversibility
OT  - physiological uncoupling
OT  - yeast mitochondria
COIS- The authors declare no conflict of interest.
EDAT- 2021/12/25 06:00
MHDA- 2021/12/25 06:01
PMCR- 2021/11/27
CRDT- 2021/12/24 01:15
PHST- 2021/09/28 00:00 [received]
PHST- 2021/11/16 00:00 [revised]
PHST- 2021/11/22 00:00 [accepted]
PHST- 2021/12/24 01:15 [entrez]
PHST- 2021/12/25 06:00 [pubmed]
PHST- 2021/12/25 06:01 [medline]
PHST- 2021/11/27 00:00 [pmc-release]
AID - life11121307 [pii]
AID - life-11-01307 [pii]
AID - 10.3390/life11121307 [doi]
PST - epublish
SO  - Life (Basel). 2021 Nov 27;11(12):1307. doi: 10.3390/life11121307.