PMID- 34954436
OWN - NLM
STAT- MEDLINE
DCOM- 20220127
LR  - 20220127
IS  - 1873-3336 (Electronic)
IS  - 0304-3894 (Linking)
VI  - 426
DP  - 2022 Mar 15
TI  - A tau class glutathione-S-transferase (OsGSTU5) confers tolerance against arsenic 
      toxicity in rice by accumulating more arsenic in root.
PG  - 128100
LID - S0304-3894(21)03069-7 [pii]
LID - 10.1016/j.jhazmat.2021.128100 [doi]
AB  - Arsenic (As) considered as one of the hazardous metalloid that hampers various 
      physiological activities in rice. To study the mechanism of As tolerance in rice, 
      one differentially expressed tau class glutathione-S-transferase (OsGSTU5) has 
      been selected and transgenic rice plants with knockdown (KD) and overexpressing 
      (OE) OsGSTU5 were generated. Our results suggested that KD lines became less 
      tolerant to As stress than WT plants, while OE lines showed enhanced tolerance to 
      As. Under As toxicity, OE and KD lines showed enhanced and reduced antioxidant 
      activities such as, SOD, PRX and catalase, respectively indicating its role in 
      ROS homeostasis. In addition, higher malondialdehyde content, poor photosynthetic 
      parameters and higher reactive oxygen species (ROS) in KD plant, suggests that 
      knockdown of OsGSTU5 renders KD plants more susceptible to oxidative damage. 
      Also, the relative expression profile of various transporters such as OsABCC1 (As 
      sequestration), Lsi2 and Lsi6 (As translocaters) and GSH dependent activity of 
      GSTU5 suggests that GSTU5 might help in chelation of As with GSH and sequester it 
      into the root vacuole using OsABCC1 transporter and thus limits the upward 
      translocation of As towards shoot. This study suggests the importance of GSTU5 as 
      a good target to improve the As tolerance in rice.
CI  - Copyright (c) 2021 Elsevier B.V. All rights reserved.
FAU - Tiwari, Madhu
AU  - Tiwari M
AD  - Biotechnology and Molecular Biology Division, CSIR-National Botanical Research 
      Institute, Lucknow 226001, India; Laboratory of Microbial Genetics, Department of 
      Botany, Banaras Hindu University, Varanasi 221005, India.
FAU - Kidwai, Maria
AU  - Kidwai M
AD  - Biotechnology and Molecular Biology Division, CSIR-National Botanical Research 
      Institute, Lucknow 226001, India.
FAU - Dutta, Prasanna
AU  - Dutta P
AD  - Biotechnology and Molecular Biology Division, CSIR-National Botanical Research 
      Institute, Lucknow 226001, India; Academy of Scientific and Innovative Research 
      (AcSIR), Ghaziabad 201002, India.
FAU - Narayan, Shiv
AU  - Narayan S
AD  - Academy of Scientific and Innovative Research (AcSIR), Ghaziabad 201002, India; 
      Plant Physiology Laboratory, CSIR-National Botanical Research Institute, 
      Lucknow 226001, India.
FAU - Gautam, Neelam
AU  - Gautam N
AD  - Biotechnology and Molecular Biology Division, CSIR-National Botanical Research 
      Institute, Lucknow 226001, India; Academy of Scientific and Innovative Research 
      (AcSIR), Ghaziabad 201002, India.
FAU - Chawda, Khushboo
AU  - Chawda K
AD  - Biotechnology and Molecular Biology Division, CSIR-National Botanical Research 
      Institute, Lucknow 226001, India; Academy of Scientific and Innovative Research 
      (AcSIR), Ghaziabad 201002, India.
FAU - Shirke, Pramod Arvind
AU  - Shirke PA
AD  - Academy of Scientific and Innovative Research (AcSIR), Ghaziabad 201002, India; 
      Plant Physiology Laboratory, CSIR-National Botanical Research Institute, 
      Lucknow 226001, India.
FAU - Mishra, Arun Kumar
AU  - Mishra AK
AD  - Laboratory of Microbial Genetics, Department of Botany, Banaras Hindu University, 
      Varanasi 221005, India.
FAU - Chakrabarty, Debasis
AU  - Chakrabarty D
AD  - Biotechnology and Molecular Biology Division, CSIR-National Botanical Research 
      Institute, Lucknow 226001, India; Academy of Scientific and Innovative Research 
      (AcSIR), Ghaziabad 201002, India. Electronic address: chakrabartyd@nbri.res.in.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20211218
PL  - Netherlands
TA  - J Hazard Mater
JT  - Journal of hazardous materials
JID - 9422688
RN  - 0 (Antioxidants)
RN  - EC 2.5.1.18 (Glutathione Transferase)
RN  - GAN16C9B8O (Glutathione)
RN  - N712M78A8G (Arsenic)
SB  - IM
MH  - Antioxidants
MH  - *Arsenic/toxicity
MH  - Glutathione
MH  - Glutathione Transferase/genetics
MH  - *Oryza/genetics
MH  - Plant Roots
OTO - NOTNLM
OT  - Arsenic
OT  - Arsenic transporters
OT  - Buthionine sulfoximine (BSO)
OT  - Glutathione-s-transferase (GST)
OT  - Rice
EDAT- 2021/12/27 06:00
MHDA- 2022/01/28 06:00
CRDT- 2021/12/26 20:44
PHST- 2021/02/05 00:00 [received]
PHST- 2021/09/03 00:00 [revised]
PHST- 2021/12/15 00:00 [accepted]
PHST- 2021/12/27 06:00 [pubmed]
PHST- 2022/01/28 06:00 [medline]
PHST- 2021/12/26 20:44 [entrez]
AID - S0304-3894(21)03069-7 [pii]
AID - 10.1016/j.jhazmat.2021.128100 [doi]
PST - ppublish
SO  - J Hazard Mater. 2022 Mar 15;426:128100. doi: 10.1016/j.jhazmat.2021.128100. Epub 
      2021 Dec 18.