PMID- 34956281 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20211228 IS - 1664-462X (Print) IS - 1664-462X (Electronic) IS - 1664-462X (Linking) VI - 12 DP - 2021 TI - Identification of a Wheat-Psathyrostachys huashanica 7Ns Ditelosomic Addition Line Conferring Early Maturation by Cytological Analysis and Newly Developed Molecular and FISH Markers. PG - 784001 LID - 10.3389/fpls.2021.784001 [doi] LID - 784001 AB - Early maturation is an important objective in wheat breeding programs that could facilitate multiple-cropping systems, decrease disaster- and disease-related losses, ensure stable wheat production, and increase economic benefits. Exploitation of novel germplasm from wild relatives of wheat is an effective means of breeding for early maturity. Psathyrostachys huashanica Keng f. ex P. C. KUO (2n=2x=14, NsNs) is a promising source of useful genes for wheat genetic improvement. In this study, we characterized a novel wheat-P. huashanica line, DT23, derived from distant hybridization between common wheat and P. huashanica. Fluorescence in situ hybridization (FISH) and sequential genomic in situ hybridization (GISH) analyses indicated that DT23 is a stable wheat-P. huashanica ditelosomic addition line. FISH painting and PCR-based landmark unique gene markers analyses further revealed that DT23 is a wheat-P. huashanica 7Ns ditelosomic addition line. Observation of spike differentiation and the growth period revealed that DT23 exhibited earlier maturation than the wheat parents. This is the first report of new earliness per se (Eps) gene(s) probably associated with a group 7 chromosome of P. huashanica. Based on specific locus-amplified fragment sequencing technology, 45 new specific molecular markers and 19 specific FISH probes were developed for the P. huashanica 7Ns chromosome. Marker validation analyses revealed that two specific markers distinguished the Ns genome chromosomes of P. huashanica and the chromosomes of other wheat-related species. These newly developed FISH probes specifically detected Ns genome chromosomes of P. huashanica in the wheat background. The DT23 line will be useful for breeding early maturing wheat. The specific markers and FISH probes developed in this study can be used to detect and trace P. huashanica chromosomes and chromosomal segments carrying elite genes in diverse materials. CI - Copyright (c) 2021 Tan, Zhao, Li, Zhang, Zhu, Xu, Wang, Zeng, Fan, Sha, Wu, Cheng, Zhang, Chen, Zhou and Kang. FAU - Tan, Binwen AU - Tan B AD - Triticeae Research Institute, Sichuan Agricultural University, Chengdu, China. AD - State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, China. FAU - Zhao, Lei AU - Zhao L AD - Triticeae Research Institute, Sichuan Agricultural University, Chengdu, China. AD - State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, China. FAU - Li, Lingyu AU - Li L AD - Triticeae Research Institute, Sichuan Agricultural University, Chengdu, China. AD - State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, China. FAU - Zhang, Hao AU - Zhang H AD - Triticeae Research Institute, Sichuan Agricultural University, Chengdu, China. AD - State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, China. FAU - Zhu, Wei AU - Zhu W AD - Triticeae Research Institute, Sichuan Agricultural University, Chengdu, China. AD - State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, China. FAU - Xu, Lili AU - Xu L AD - Triticeae Research Institute, Sichuan Agricultural University, Chengdu, China. FAU - Wang, Yi AU - Wang Y AD - Triticeae Research Institute, Sichuan Agricultural University, Chengdu, China. AD - State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, China. FAU - Zeng, Jian AU - Zeng J AD - College of Resources, Sichuan Agricultural University, Chengdu, China. FAU - Fan, Xing AU - Fan X AD - Triticeae Research Institute, Sichuan Agricultural University, Chengdu, China. AD - State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, China. FAU - Sha, Lina AU - Sha L AD - College of Grassland Science and Technology, Sichuan Agricultural University, Chengdu, China. FAU - Wu, Dandan AU - Wu D AD - Triticeae Research Institute, Sichuan Agricultural University, Chengdu, China. AD - State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, China. FAU - Cheng, Yiran AU - Cheng Y AD - State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, China. FAU - Zhang, Haiqin AU - Zhang H AD - College of Grassland Science and Technology, Sichuan Agricultural University, Chengdu, China. FAU - Chen, Guoyue AU - Chen G AD - Triticeae Research Institute, Sichuan Agricultural University, Chengdu, China. AD - State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, China. FAU - Zhou, Yonghong AU - Zhou Y AD - Triticeae Research Institute, Sichuan Agricultural University, Chengdu, China. AD - State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, China. FAU - Kang, Houyang AU - Kang H AD - Triticeae Research Institute, Sichuan Agricultural University, Chengdu, China. AD - State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, China. LA - eng PT - Journal Article DEP - 20211209 PL - Switzerland TA - Front Plant Sci JT - Frontiers in plant science JID - 101568200 PMC - PMC8695443 OTO - NOTNLM OT - FISH probes OT - Psathyrostachys huashanica OT - SLAF-seq OT - ditelosomic addition line OT - early maturation OT - molecular marker COIS- The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2021/12/28 06:00 MHDA- 2021/12/28 06:01 PMCR- 2021/01/01 CRDT- 2021/12/27 06:25 PHST- 2021/09/27 00:00 [received] PHST- 2021/11/12 00:00 [accepted] PHST- 2021/12/27 06:25 [entrez] PHST- 2021/12/28 06:00 [pubmed] PHST- 2021/12/28 06:01 [medline] PHST- 2021/01/01 00:00 [pmc-release] AID - 10.3389/fpls.2021.784001 [doi] PST - epublish SO - Front Plant Sci. 2021 Dec 9;12:784001. doi: 10.3389/fpls.2021.784001. eCollection 2021.