PMID- 34957181
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20211228
IS  - 2296-861X (Print)
IS  - 2296-861X (Electronic)
IS  - 2296-861X (Linking)
VI  - 8
DP  - 2021
TI  - Determination of 35 Free Amino Acids in Tea Using Ultra-Performance Liquid 
      Chromatography Coupled With Quadrupole Time-of-Flight Mass Spectrometry.
PG  - 767801
LID - 10.3389/fnut.2021.767801 [doi]
LID - 767801
AB  - The purpose of this research was to develop a simple, sensitive, and accurate 
      method for simultaneous determination of 35 free amino acids using 
      ultra-performance liquid chromatography coupled with quadrupole time-of-flight 
      mass spectrometry (UPLC-Q-TOF/MS). Tea samples were extracted with boiling water 
      bath, and then separated by XBridge BEH Amide column by gradient elution. The 
      exact mass and MS/MS spectra of the target compound was detected under the TOF-MS 
      and Information dependent acquisition (IDA)-MS/MS mode. The results demonstrated 
      good linearity (R (2) > 0.9980) in the range of 0.5-1,000 ng/mL. The limits of 
      detection (LODs) were 0.13-25.00 mg/kg and the limits of quantitation (LOQs) were 
      0.25-50.00 mg/kg. The recovery rate ranged from 70.1 to 105.1% with relative 
      standard deviations (RSDs) <11% (n = 6). This research provides a targeted 
      strategy for developing an analysis method for amino acids in tea.
CI  - Copyright (c) 2021 Li, Ma, Li, Fan, Fan, Ma, Zhang and Zheng.
FAU - Li, Jian
AU  - Li J
AD  - School of Food and Biological Engineering, Hefei University of Technology, Hefei, 
      China.
AD  - College of Applied Arts and Science, Beijing Union University, Beijing, China.
FAU - Ma, Junmei
AU  - Ma J
AD  - Hebei Food Safety Key Laboratory, Hebei Food Inspection and Research Institute, 
      Shijiazhuang, China.
AD  - Hebei Key Laboratory of Forensic Medicine, College of Forensic Medicine, Hebei 
      Medical University, Shijiazhuang, China.
FAU - Li, Qiang
AU  - Li Q
AD  - Hebei Food Safety Key Laboratory, Hebei Food Inspection and Research Institute, 
      Shijiazhuang, China.
FAU - Fan, Sufang
AU  - Fan S
AD  - Hebei Food Safety Key Laboratory, Hebei Food Inspection and Research Institute, 
      Shijiazhuang, China.
FAU - Fan, Lixin
AU  - Fan L
AD  - Hebei Food Safety Key Laboratory, Hebei Food Inspection and Research Institute, 
      Shijiazhuang, China.
FAU - Ma, Hongyu
AU  - Ma H
AD  - Hebei Food Safety Key Laboratory, Hebei Food Inspection and Research Institute, 
      Shijiazhuang, China.
FAU - Zhang, Yan
AU  - Zhang Y
AD  - Hebei Food Safety Key Laboratory, Hebei Food Inspection and Research Institute, 
      Shijiazhuang, China.
AD  - Hebei Key Laboratory of Forensic Medicine, College of Forensic Medicine, Hebei 
      Medical University, Shijiazhuang, China.
FAU - Zheng, Lei
AU  - Zheng L
AD  - School of Food and Biological Engineering, Hefei University of Technology, Hefei, 
      China.
LA  - eng
PT  - Journal Article
DEP - 20211201
PL  - Switzerland
TA  - Front Nutr
JT  - Frontiers in nutrition
JID - 101642264
PMC - PMC8697017
OTO - NOTNLM
OT  - detection
OT  - free amino acids
OT  - quadrupole-time of flight mass spectrometry
OT  - tea
OT  - ultra-performance liquid chromatography
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2021/12/28 06:00
MHDA- 2021/12/28 06:01
PMCR- 2021/01/01
CRDT- 2021/12/27 06:34
PHST- 2021/08/31 00:00 [received]
PHST- 2021/10/29 00:00 [accepted]
PHST- 2021/12/27 06:34 [entrez]
PHST- 2021/12/28 06:00 [pubmed]
PHST- 2021/12/28 06:01 [medline]
PHST- 2021/01/01 00:00 [pmc-release]
AID - 10.3389/fnut.2021.767801 [doi]
PST - epublish
SO  - Front Nutr. 2021 Dec 1;8:767801. doi: 10.3389/fnut.2021.767801. eCollection 2021.