PMID- 34957219
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20211228
IS  - 2296-889X (Print)
IS  - 2296-889X (Electronic)
IS  - 2296-889X (Linking)
VI  - 8
DP  - 2021
TI  - Impact of the Glycemic Level on the Salivary Proteome of Middle-Aged and Elderly 
      People With Type 2 Diabetes Mellitus: An Observational Study.
PG  - 790091
LID - 10.3389/fmolb.2021.790091 [doi]
LID - 790091
AB  - Type 2 diabetes mellitus (T2DM) is an increasing global public health concern, 
      but its impact on the salivary proteome is still unclear. To evaluate the effect 
      of glycemic levels in middle-aged and elderly individuals with T2DM on salivary 
      proteomics, we compared the differences by liquid chromatography tandem mass 
      spectrometry (LC-MS/MS). Unstimulated whole saliva samples from 8 T2DM patients 
      with good glycemic control (G group, HbA1c <6.5%) and 16 patients with poor 
      control (P group, HbA1c >/=6.5%) were analyzed by LC-MS/MS in the data-independent 
      acquisition mode (Clinical register number: ChiCTR1900023582.). After functional 
      annotation, cluster analysis and receiver operating characteristic (ROC) curve 
      analysis were carried out to screen and evaluate candidate proteins. A total of 
      5,721 proteins were quantified, while 40 proteins differed significantly. In the 
      P group, proteins involved in oxidative stress-related processes were 
      upregulated, whereas proteins related to salivary secretion were downregulated. 
      The combination of thioredoxin domain-containing protein 17, zymogen granule 
      protein 16B, and FAM3 metabolism regulating signaling molecule D yielded an area 
      under the curve of 0.917 which showed a robust ability to distinguish the P and G 
      groups. In conclusion, poorly controlled hyperglycemia may affect salivary 
      proteins through various pathways, including oxidative stress and glandular 
      secretion. Furthermore, the differentially expressed proteins, especially the 
      three proteins with the best differentiation, might serve as an anchor point for 
      the further study of hyperglycemia and oral diseases.
CI  - Copyright (c) 2021 Jia, Zhang, Sun, Yuan and Zheng.
FAU - Jia, Shu Yuan
AU  - Jia SY
AD  - Department of Preventive Dentistry, Peking University School and Hospital of 
      Stomatology, National Center of Stomatology, National Clinical Research Center 
      for Oral Diseases, National Engineering Laboratory for Digital and Material 
      Technology of Stomatology, Beijing, China.
FAU - Zhang, Yan Ling
AU  - Zhang YL
AD  - Department of Periodontology, Peking University School and Hospital of 
      Stomatology, National Center of Stomatology, National Clinical Research Center 
      for Oral Diseases, National Engineering Laboratory for Digital and Material 
      Technology of Stomatology, Beijing, China.
FAU - Sun, Xiang Yu
AU  - Sun XY
AD  - Department of Preventive Dentistry, Peking University School and Hospital of 
      Stomatology, National Center of Stomatology, National Clinical Research Center 
      for Oral Diseases, National Engineering Laboratory for Digital and Material 
      Technology of Stomatology, Beijing, China.
FAU - Yuan, Chao
AU  - Yuan C
AD  - Department of Preventive Dentistry, Peking University School and Hospital of 
      Stomatology, National Center of Stomatology, National Clinical Research Center 
      for Oral Diseases, National Engineering Laboratory for Digital and Material 
      Technology of Stomatology, Beijing, China.
FAU - Zheng, Shu Guo
AU  - Zheng SG
AD  - Department of Preventive Dentistry, Peking University School and Hospital of 
      Stomatology, National Center of Stomatology, National Clinical Research Center 
      for Oral Diseases, National Engineering Laboratory for Digital and Material 
      Technology of Stomatology, Beijing, China.
LA  - eng
PT  - Journal Article
DEP - 20211210
PL  - Switzerland
TA  - Front Mol Biosci
JT  - Frontiers in molecular biosciences
JID - 101653173
PMC - PMC8703016
OTO - NOTNLM
OT  - Data-independent acquisition
OT  - mass spectrometry
OT  - oxidative stress
OT  - salivary proteome
OT  - salivary secretion
OT  - type 2 diabetes mellitus
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2021/12/28 06:00
MHDA- 2021/12/28 06:01
PMCR- 2021/01/01
CRDT- 2021/12/27 06:34
PHST- 2021/10/06 00:00 [received]
PHST- 2021/11/22 00:00 [accepted]
PHST- 2021/12/27 06:34 [entrez]
PHST- 2021/12/28 06:00 [pubmed]
PHST- 2021/12/28 06:01 [medline]
PHST- 2021/01/01 00:00 [pmc-release]
AID - 790091 [pii]
AID - 10.3389/fmolb.2021.790091 [doi]
PST - epublish
SO  - Front Mol Biosci. 2021 Dec 10;8:790091. doi: 10.3389/fmolb.2021.790091. 
      eCollection 2021.