PMID- 34959237
OWN - NLM
STAT- MEDLINE
DCOM- 20220405
LR  - 20220502
IS  - 1421-9859 (Electronic)
IS  - 0378-5866 (Linking)
VI  - 44
IP  - 2
DP  - 2022
TI  - Bioinformatic Analysis Reveals the Distinct Role of 5'UTR-Specific m6A RNA 
      Modification in Mice Developing Cerebral Cortices.
PG  - 67-79
LID - 10.1159/000521620 [doi]
AB  - N6-methyladenosine (m6A) abundantly exists in the cerebral cortex and is emerging 
      as an essential factor in cortical development and function. As the m6A-binding 
      site appears to be dynamically methylated in different RNA regions at the 
      temporal-specific developing stage, it is of value to distinguish the unique 
      character of region- and temporal-specific m6A. Herein, we analyzed the status of 
      temporal-specific m6A within RNA 5' untranslated region (5'UTR) using 
      m6A-methylated sequencing data and transcriptomic sequencing data from 12.5- to 
      13-day embryonic cerebral cortices and 14-day postnatal ones. We identified sorts 
      of RNAs that are uniquely m6A-methylated in the 5'UTR and sorted them into 
      specific neurological processes. Compared with 3'UTR-m6A-methylated RNAs, 
      5'UTR-m6A-methylated RNAs showed unique functions and mechanisms in regulating 
      cortical development, especially through the pathway of mRNA transport and 
      surveillance. Moreover, the 5'UTR-specific m6A was associated with neurological 
      disorders as well. The FoxO signaling pathway was then focused by these 
      pathogenic 5'UTR-m6A-methylated RNAs and explored to be involved in the 
      determination of neurological disorders. Additionally, the 5'UTR-m6A modification 
      patterns and transcriptional patterns play independent but cohesive roles in the 
      developing cortices. Our study emphasizes the importance of 5'UTR-specific m6A in 
      the developing cortex and provides an informative reference for future studies of 
      5'UTR-specific m6A in normal cortical development and neurological disorders.
CI  - (c) 2021 S. Karger AG, Basel.
FAU - Zhang, Long-Bin
AU  - Zhang LB
AD  - Fujian Key Laboratory of Marine Enzyme Engineering, Fuzhou University, Fujian, 
      China.
FAU - Qiu, Ting-Ting
AU  - Qiu TT
AD  - Fujian Key Laboratory of Marine Enzyme Engineering, Fuzhou University, Fujian, 
      China.
FAU - Yang, Wu-Wei-Jie
AU  - Yang WW
AD  - Fujian Key Laboratory of Marine Enzyme Engineering, Fuzhou University, Fujian, 
      China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20211227
PL  - Switzerland
TA  - Dev Neurosci
JT  - Developmental neuroscience
JID - 7809375
RN  - 0 (5' Untranslated Regions)
RN  - K72T3FS567 (Adenosine)
SB  - IM
MH  - 5' Untranslated Regions/genetics
MH  - *Adenosine/metabolism
MH  - Animals
MH  - Cerebral Cortex/metabolism
MH  - *Computational Biology
MH  - Mice
OTO - NOTNLM
OT  - Cerebral cortex
OT  - Embryonic stage
OT  - Neurological disorders
OT  - Postnatal stage
OT  - RNA 5'UTR
OT  - m6A RNA methylation
EDAT- 2021/12/28 06:00
MHDA- 2022/04/06 06:00
CRDT- 2021/12/27 20:28
PHST- 2021/09/16 00:00 [received]
PHST- 2021/12/21 00:00 [accepted]
PHST- 2021/12/28 06:00 [pubmed]
PHST- 2022/04/06 06:00 [medline]
PHST- 2021/12/27 20:28 [entrez]
AID - 000521620 [pii]
AID - 10.1159/000521620 [doi]
PST - ppublish
SO  - Dev Neurosci. 2022;44(2):67-79. doi: 10.1159/000521620. Epub 2021 Dec 27.