PMID- 34959807
OWN - NLM
STAT- MEDLINE
DCOM- 20220118
LR  - 20220118
IS  - 2072-6643 (Electronic)
IS  - 2072-6643 (Linking)
VI  - 13
IP  - 12
DP  - 2021 Nov 26
TI  - Human Milk Oligosaccharides in Cord Blood Are Altered in Gestational Diabetes and 
      Stimulate Feto-Placental Angiogenesis In Vitro.
LID - 10.3390/nu13124257 [doi]
LID - 4257
AB  - (1) Background: Human milk oligosaccharides (HMOs) are present in maternal serum 
      during pregnancy and their composition is altered in gestational diabetes (GDM). 
      HMOs are also in fetal cord blood and in contact with the feto-placental 
      endothelium, potentially affecting its functions, such as angiogenesis. We 
      hypothesized that cord blood HMOs are changed in GDM and contribute to increased 
      feto-placental angiogenesis, hallmark of GDM. (2) Methods: Using HPLC, we 
      quantified HMOs in cord blood of women with normal glucose tolerance (NGT, n = 
      25) or GDM (n = 26). We investigated in vitro angiogenesis using primary 
      feto-placental endothelial cells (fpECs) from term placentas after healthy 
      pregnancy (n = 10), in presence or absence of HMOs (100 microg/mL) isolated from 
      human milk, 3'-sialyllactose (3'SL, 30 microg/mL) and lactose (glycan control) and 
      determined network formation (Matrigel assay), proliferation (MTT assays), actin 
      organization (F-actin staining), tube formation (fibrin tube formation assay) and 
      sprouting (spheroid sprouting assay). (3) Results: 3'SL was higher in GDM cord 
      blood. HMOs increased network formation, HMOs and 3'SL increased proliferation 
      and F-actin staining. In fibrin assays, HMOs and 3'SL increased total tube length 
      by 24% and 25% (p < 0.05), in spheroid assays, by 32% (p < 0.05) and 21% (p = 
      0.056), respectively. Lactose had no effect. (4) Conclusions: Our study suggests 
      a novel role of HMOs in feto-placental angiogenesis and indicates a contribution 
      of HMO composition to altered feto-placental vascularization in GDM.
FAU - Hoch, Denise
AU  - Hoch D
AUID- ORCID: 0000-0002-7169-7007
AD  - Department of Obstetrics and Gynecology, Medical University of Graz, 8036 Graz, 
      Austria.
FAU - Brandl, Waltraud
AU  - Brandl W
AD  - Department of Obstetrics and Gynecology, Medical University of Graz, 8036 Graz, 
      Austria.
FAU - Strutz, Jasmin
AU  - Strutz J
AUID- ORCID: 0000-0002-6138-7566
AD  - Department of Obstetrics and Gynecology, Medical University of Graz, 8036 Graz, 
      Austria.
AD  - Institute of Biomedical Science, Carinthia University of Applied Science, 9020 
      Klagenfurt, Austria.
FAU - Kofeler, Harald C
AU  - Kofeler HC
AD  - Core Facility Mass Spectrometry, Center for Medical Research, Medical University 
      of Graz, 8010 Graz, Austria.
AD  - BioTechMed Graz, 8010 Graz, Austria.
FAU - van Poppel, Mireille N M
AU  - van Poppel MNM
AUID- ORCID: 0000-0001-5694-4324
AD  - BioTechMed Graz, 8010 Graz, Austria.
AD  - Institute of Human Movement Science, Sport and Health, University of Graz, 8010 
      Graz, Austria.
FAU - Bode, Lars
AU  - Bode L
AD  - Department of Pediatrics and Larsson-Rosenquist Foundation Mother-Milk-Infant 
      Center of Research Excellence (LRF MoMI CoRE), University of California San 
      Diego, La Jolla, CA 92093, USA.
FAU - Hiden, Ursula
AU  - Hiden U
AD  - Department of Obstetrics and Gynecology, Medical University of Graz, 8036 Graz, 
      Austria.
FAU - Desoye, Gernot
AU  - Desoye G
AUID- ORCID: 0000-0002-5715-3230
AD  - Department of Obstetrics and Gynecology, Medical University of Graz, 8036 Graz, 
      Austria.
FAU - Jantscher-Krenn, Evelyn
AU  - Jantscher-Krenn E
AUID- ORCID: 0000-0003-3568-891X
AD  - Department of Obstetrics and Gynecology, Medical University of Graz, 8036 Graz, 
      Austria.
AD  - BioTechMed Graz, 8010 Graz, Austria.
LA  - eng
GR  - 627056/European Commission/
GR  - 16927/National Bank of Austria/
GR  - KLI 784/FWF Austrian Science Fund/
PT  - Journal Article
DEP - 20211126
PL  - Switzerland
TA  - Nutrients
JT  - Nutrients
JID - 101521595
RN  - 0 (3'-sialyllactose)
RN  - 0 (Angiogenesis Inducing Agents)
RN  - 0 (Oligosaccharides)
RN  - J2B2A4N98G (Lactose)
SB  - IM
MH  - Angiogenesis Inducing Agents/*blood
MH  - Cell Proliferation/drug effects
MH  - Chromatography, High Pressure Liquid
MH  - Diabetes, Gestational/*blood
MH  - Endothelial Cells/chemistry
MH  - Female
MH  - Fetal Blood/*chemistry
MH  - Humans
MH  - Lactose/blood
MH  - Milk, Human/chemistry
MH  - Oligosaccharides/*blood
MH  - Placenta/blood supply/cytology
MH  - Placental Circulation/*drug effects
MH  - Pregnancy
PMC - PMC8705424
OTO - NOTNLM
OT  - 3'-sialyllactose
OT  - angiogenesis
OT  - cord blood
OT  - fibrin tube formation assay
OT  - gestational diabetes mellitus
OT  - human milk oligosaccharides
OT  - placenta
OT  - pregnancy
OT  - spheroid sprouting assay
OT  - tube formation
COIS- The authors declare no conflict of interest.
EDAT- 2021/12/29 06:00
MHDA- 2022/01/19 06:00
PMCR- 2021/11/26
CRDT- 2021/12/28 01:04
PHST- 2021/10/27 00:00 [received]
PHST- 2021/11/23 00:00 [revised]
PHST- 2021/11/24 00:00 [accepted]
PHST- 2021/12/28 01:04 [entrez]
PHST- 2021/12/29 06:00 [pubmed]
PHST- 2022/01/19 06:00 [medline]
PHST- 2021/11/26 00:00 [pmc-release]
AID - nu13124257 [pii]
AID - nutrients-13-04257 [pii]
AID - 10.3390/nu13124257 [doi]
PST - epublish
SO  - Nutrients. 2021 Nov 26;13(12):4257. doi: 10.3390/nu13124257.