PMID- 34961172
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20240404
IS  - 2223-7747 (Print)
IS  - 2223-7747 (Electronic)
IS  - 2223-7747 (Linking)
VI  - 10
IP  - 12
DP  - 2021 Dec 8
TI  - Chitosan Nanoparticles Inactivate Alfalfa Mosaic Virus Replication and Boost 
      Innate Immunity in Nicotiana glutinosa Plants.
LID - 10.3390/plants10122701 [doi]
LID - 2701
AB  - Plant viral infection is one of the most severe issues in food security globally, 
      resulting in considerable crop production losses. Chitosan is a well-known 
      biocontrol agent against a variety of plant infections. However, research on 
      combatting viral infections is still in its early stages. The current study 
      investigated the antiviral activities (protective, curative, and inactivation) of 
      the prepared chitosan/dextran nanoparticles (CDNPs, 100 microg mL(-1)) on Nicotiana 
      glutinosa plants. Scanning electron microscope (SEM) and dynamic light scattering 
      analysis revealed that the synthesized CDNPs had a uniform, regular sphere shapes 
      ranging from 20 to 160 nm in diameter, with an average diameter of 91.68 nm. The 
      inactivation treatment was the most effective treatment, which resulted in a 100% 
      reduction in the alfalfa mosaic virus (AMV, Acc# OK413670) accumulation level. On 
      the other hand, the foliar application of CDNPs decreased disease severity and 
      significantly reduced viral accumulation levels by 70.43% and 61.65% in 
      protective and curative treatments, respectively, under greenhouse conditions. 
      Additionally, the induction of systemic acquired resistance, increasing total 
      carbohydrates and total phenolic contents, as well as triggering the 
      transcriptional levels of peroxidase, pathogen-related protein-1, and 
      phenylalanine ammonia-lyase were observed. In light of the results, we propose 
      that the potential application of CDNPs could be an eco-friendly approach to 
      enhance yield and a more effective therapeutic elicitor for disease management in 
      plants upon induction of defense systems.
FAU - Abdelkhalek, Ahmed
AU  - Abdelkhalek A
AUID- ORCID: 0000-0002-8078-3265
AD  - Plant Protection and Biomolecular Diagnosis Department, ALCRI, City of Scientific 
      Research and Technological Applications, New Borg El-Arab City 21934, Alexandria, 
      Egypt.
FAU - Qari, Sameer H
AU  - Qari SH
AUID- ORCID: 0000-0001-9424-5217
AD  - Biology Department, Al-Jumum University College, Umm Al-Qura University, Mecca 
      25376, Saudi Arabia.
FAU - Abu-Saied, Mohamed Abd Al-Raheem
AU  - Abu-Saied MAA
AD  - Polymeric Materials Research Department, Advanced Technology and New Materials 
      Research Institute, City of Scientific Research and Technological Applications 
      (SRTA-City), New Borg El-Arab City 21934, Alexandria, Egypt.
FAU - Khalil, Abdallah Mohamed
AU  - Khalil AM
AD  - Plant Botany Department, Faculty of Science, Omar Al-Mukhtar University, Al Bayda 
      00218-84, Libya.
FAU - Younes, Hosny A
AU  - Younes HA
AD  - Agricultural Botany Department, Faculty of Agriculture (Saba Basha), Alexandria 
      University, Alexandria 21531, Egypt.
FAU - Nehela, Yasser
AU  - Nehela Y
AUID- ORCID: 0000-0003-1177-8099
AD  - Department of Agricultural Botany, Faculty of Agriculture, Tanta University, 
      Tanta 31511, Egypt.
AD  - Citrus Research and Education Center, Department of Plant Pathology, University 
      of Florida, 700 Experiment Station Rd., Lake Alfred, FL 33850, USA.
FAU - Behiry, Said I
AU  - Behiry SI
AUID- ORCID: 0000-0001-5701-2254
AD  - Agricultural Botany Department, Faculty of Agriculture (Saba Basha), Alexandria 
      University, Alexandria 21531, Egypt.
LA  - eng
PT  - Journal Article
DEP - 20211208
PL  - Switzerland
TA  - Plants (Basel)
JT  - Plants (Basel, Switzerland)
JID - 101596181
PMC - PMC8703458
OTO - NOTNLM
OT  - alfalfa mosaic virus
OT  - antiviral activity
OT  - chitosan nanoparticles
OT  - gene expression
COIS- The authors declare no conflict of interest.
EDAT- 2021/12/29 06:00
MHDA- 2021/12/29 06:01
PMCR- 2021/12/08
CRDT- 2021/12/28 01:09
PHST- 2021/11/05 00:00 [received]
PHST- 2021/11/26 00:00 [revised]
PHST- 2021/12/03 00:00 [accepted]
PHST- 2021/12/28 01:09 [entrez]
PHST- 2021/12/29 06:00 [pubmed]
PHST- 2021/12/29 06:01 [medline]
PHST- 2021/12/08 00:00 [pmc-release]
AID - plants10122701 [pii]
AID - plants-10-02701 [pii]
AID - 10.3390/plants10122701 [doi]
PST - epublish
SO  - Plants (Basel). 2021 Dec 8;10(12):2701. doi: 10.3390/plants10122701.