PMID- 34962099
OWN - NLM
STAT- MEDLINE
DCOM- 20220427
LR  - 20220427
IS  - 1616-5195 (Electronic)
IS  - 1616-5187 (Linking)
VI  - 22
IP  - 3
DP  - 2022 Mar
TI  - Enhanced Bone Regeneration Using a ZIF-8-Loaded Fibrin Composite Scaffold.
PG  - e2100416
LID - 10.1002/mabi.202100416 [doi]
AB  - In the present study, fibrin-based biomaterials made of zeolite imidazole 
      framework-8 (ZIF-8) and fibrin gel (Z-FG) are fabricated with the aim of 
      enhancing skull regeneration. X-ray diffraction (XRD), scanning electron 
      microscopy (SEM), ultraviolet (UV)-vis spectrophotometry, Fourier transform 
      infrared spectroscopy, and rheometry are used to characterize ZIF-8 and Z-FG. The 
      influences of ZIF-8 on the physical properties of fibrin gel (e.g., porosity, 
      modulus, and in vitro biodegradation) are investigated, and the effect of ZIF-8 
      concentration on fibrin gel properties in vitro is determined by seeding 
      ectomesenchymal stem cells (EMSCs) over Z-FG. EMSC osteogenic differentiation 
      reveals higher expression of bone-related proteins and higher calcium deposition 
      and alkaline phosphatase activity, indicating that Z-FG may be a good 
      osteoinductive biomaterial. Furthermore, these results show that the piezochannel 
      and yes-associated protein (YAP) signaling pathway are involved in the 
      differentiation process. In addition, the in vivo results demonstrate that Z-FG 
      increases bone formation in critical-sized calvarial defects in rats. Thus, the 
      developed composite scaffold may be a suitable biomaterial for skull 
      tissue-engineering applications.
CI  - (c) 2022 Wiley-VCH GmbH.
FAU - Shi, Wentao
AU  - Shi W
AUID- ORCID: 0000-0001-8451-5850
AD  - Jiangnan University Affiliated Hospital, Jiangnan University, Wuxi, Jiangsu 
      Province, 214122, P. R. China.
AD  - Jiangnan University Neuroscience Center, Jiangnan University, Wuxi, Jiangsu 
      Province, 214122, P. R. China.
FAU - Bian, Lu
AU  - Bian L
AD  - Jiangnan University Affiliated Hospital, Jiangnan University, Wuxi, Jiangsu 
      Province, 214122, P. R. China.
AD  - School of Medicine, Jiangnan University, Wuxi, Jiangsu Province, 214122, P. R. 
      China.
FAU - Wu, Yiqing
AU  - Wu Y
AD  - Jiangnan University Affiliated Hospital, Jiangnan University, Wuxi, Jiangsu 
      Province, 214122, P. R. China.
AD  - Jiangnan University Neuroscience Center, Jiangnan University, Wuxi, Jiangsu 
      Province, 214122, P. R. China.
FAU - Wang, Zhe
AU  - Wang Z
AD  - School of Medicine, Jiangsu University, Zhenjiang, Jiangsu Province, 212001, P. 
      R. China.
FAU - Dai, Yao
AU  - Dai Y
AD  - School of Medicine, Jiangsu University, Zhenjiang, Jiangsu Province, 212001, P. 
      R. China.
FAU - Zhou, Yanjun
AU  - Zhou Y
AD  - Jiangnan University Affiliated Hospital, Jiangnan University, Wuxi, Jiangsu 
      Province, 214122, P. R. China.
AD  - School of Medicine, Jiangnan University, Wuxi, Jiangsu Province, 214122, P. R. 
      China.
FAU - Meng, Pengfei
AU  - Meng P
AD  - Jiangnan University Affiliated Hospital, Jiangnan University, Wuxi, Jiangsu 
      Province, 214122, P. R. China.
AD  - School of Medicine, Jiangnan University, Wuxi, Jiangsu Province, 214122, P. R. 
      China.
FAU - Wang, Qing
AU  - Wang Q
AD  - Affiliated Wuxi Second Hospital, Nanjing Medical University, Wuxi, Jiangsu 
      Province, 214122, P. R. China.
AD  - Affiliated Wuxi Clinical Medicine, Nantong University, Wuxi, Jiangsu Province, 
      214122, P. R. China.
FAU - Zhang, Zhijian
AU  - Zhang Z
AD  - School of Medicine, Jiangsu University, Zhenjiang, Jiangsu Province, 212001, P. 
      R. China.
FAU - Zhao, Xijiang
AU  - Zhao X
AD  - Affiliated Wuxi Second Hospital, Nanjing Medical University, Wuxi, Jiangsu 
      Province, 214122, P. R. China.
FAU - Zhao, Peng
AU  - Zhao P
AD  - Jiangnan University Affiliated Hospital, Jiangnan University, Wuxi, Jiangsu 
      Province, 214122, P. R. China.
FAU - Lu, Xiaojie
AU  - Lu X
AD  - Jiangnan University Affiliated Hospital, Jiangnan University, Wuxi, Jiangsu 
      Province, 214122, P. R. China.
AD  - Jiangnan University Neuroscience Center, Jiangnan University, Wuxi, Jiangsu 
      Province, 214122, P. R. China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20220111
PL  - Germany
TA  - Macromol Biosci
JT  - Macromolecular bioscience
JID - 101135941
RN  - 0 (Biocompatible Materials)
RN  - 0 (Imidazoles)
RN  - 1318-02-1 (Zeolites)
RN  - 9001-31-4 (Fibrin)
SB  - IM
MH  - Animals
MH  - Biocompatible Materials/chemistry
MH  - Bone Regeneration
MH  - Cell Differentiation
MH  - Fibrin/chemistry/pharmacology
MH  - Imidazoles
MH  - *Osteogenesis
MH  - Rats
MH  - Skull
MH  - Tissue Engineering/methods
MH  - Tissue Scaffolds/chemistry
MH  - *Zeolites/pharmacology
OTO - NOTNLM
OT  - MOFs
OT  - ZIF-8
OT  - bone defects
OT  - fibrin scaffolds
OT  - osteogenic differentiation
EDAT- 2021/12/29 06:00
MHDA- 2022/04/28 06:00
CRDT- 2021/12/28 06:57
PHST- 2021/12/01 00:00 [revised]
PHST- 2021/10/12 00:00 [received]
PHST- 2021/12/29 06:00 [pubmed]
PHST- 2022/04/28 06:00 [medline]
PHST- 2021/12/28 06:57 [entrez]
AID - 10.1002/mabi.202100416 [doi]
PST - ppublish
SO  - Macromol Biosci. 2022 Mar;22(3):e2100416. doi: 10.1002/mabi.202100416. Epub 2022 
      Jan 11.