PMID- 34962345
OWN - NLM
STAT- MEDLINE
DCOM- 20220426
LR  - 20220426
IS  - 2410-8650 (Electronic)
IS  - 1607-551X (Linking)
VI  - 38
IP  - 5
DP  - 2022 May
TI  - Astragalus polysaccharide alleviated hepatocyte senescence via autophagy pathway.
PG  - 457-468
LID - 10.1002/kjm2.12495 [doi]
AB  - Aging is characterized by inevitable organ function decline over time, with 
      consequent body deterioration and increased susceptibility to death. Astragalus 
      polysaccharide (APS) has been reported to have anti-oxidative, anti-apoptotic, 
      and anti-inflammatory properties. We investigated the potential protective 
      effects of APS on hydrogen peroxide (H(2) O(2) ) induced hepatocyte senescence 
      and identified related mechanisms in L02, Huh7, and LM3 cell lines. Aged female 
      C57BL/6 mice were given APS for 1 week by intraperitoneal injection, and APS 
      provided the strongest protective effect against H(2) O(2) -induced damage at 
      100 muM. APS reduced the expression of cell senescence markers and alleviated 
      pathological damage in aged mouse liver. APS treatment decreased oxidative 
      stress, apoptosis, NOD-like receptor protein-3-mediated pyroptosis, and 
      maintained mitochondrial homeostasis. Notably, the protective effect of APS was 
      weakened in the presence of chloroquine. APS might enrich autophagy by activating 
      AMP-activated protein kinase (AMPK) and inhibiting mammalian target of rapamycin 
      (mTOR). In conclusion, APS reduced reactive oxygen species levels, inhibited 
      apoptosis and pyroptosis, and promoted mitophagy via AMPK/mTOR pathway to 
      alleviate hepatocyte senescence in vitro and in vivo.
CI  - (c) 2021 The Authors. The Kaohsiung Journal of Medical Sciences published by John 
      Wiley & Sons Australia, Ltd on behalf of Kaohsiung Medical University.
FAU - Yao, Ting
AU  - Yao T
AUID- ORCID: 0000-0003-0132-8027
AD  - Department of Infectious Diseases, Shanghai Jiao Tong University Affiliated Sixth 
      People's Hospital, Shanghai, China.
FAU - Chen, Jin-Mei
AU  - Chen JM
AD  - Department of Infectious Diseases, Shanghai Jiao Tong University Affiliated Sixth 
      People's Hospital, Shanghai, China.
FAU - Shen, Le-Er
AU  - Shen LE
AD  - Department of Infectious Diseases, Shanghai Jiao Tong University Affiliated Sixth 
      People's Hospital, Shanghai, China.
FAU - Yu, Yong-Sheng
AU  - Yu YS
AD  - Department of Infectious Diseases, Shanghai Jiao Tong University Affiliated Sixth 
      People's Hospital, Shanghai, China.
FAU - Tang, Zheng-Hao
AU  - Tang ZH
AD  - Department of Infectious Diseases, Shanghai Jiao Tong University Affiliated Sixth 
      People's Hospital, Shanghai, China.
FAU - Zang, Guo-Qing
AU  - Zang GQ
AD  - Department of Infectious Diseases, Shanghai Jiao Tong University Affiliated Sixth 
      People's Hospital, Shanghai, China.
FAU - Zhang, Yi
AU  - Zhang Y
AD  - Department of Infectious Diseases, Shanghai Jiao Tong University Affiliated Sixth 
      People's Hospital, Shanghai, China.
FAU - Chen, Xiao-Hua
AU  - Chen XH
AD  - Department of Infectious Diseases, Shanghai Jiao Tong University Affiliated Sixth 
      People's Hospital, Shanghai, China.
LA  - eng
GR  - 81770589/National Natural Science Foundation of China/
PT  - Journal Article
DEP - 20211228
PL  - China (Republic : 1949- )
TA  - Kaohsiung J Med Sci
JT  - The Kaohsiung journal of medical sciences
JID - 100960562
RN  - 0 (Polysaccharides)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
RN  - EC 2.7.11.31 (AMP-Activated Protein Kinases)
SB  - IM
MH  - *AMP-Activated Protein Kinases/genetics/metabolism
MH  - Animals
MH  - Apoptosis
MH  - *Astragalus Plant/metabolism
MH  - Autophagy
MH  - Hepatocytes/metabolism
MH  - Mammals/metabolism
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Polysaccharides/pharmacology
MH  - Signal Transduction
MH  - TOR Serine-Threonine Kinases/genetics/metabolism
OTO - NOTNLM
OT  - AMPK
OT  - Astragalus polysaccharide
OT  - autophagy
OT  - cell death
OT  - hepatocyte senescence
EDAT- 2021/12/29 06:00
MHDA- 2022/04/27 06:00
CRDT- 2021/12/28 08:55
PHST- 2021/10/10 00:00 [revised]
PHST- 2021/08/15 00:00 [received]
PHST- 2021/12/08 00:00 [accepted]
PHST- 2021/12/29 06:00 [pubmed]
PHST- 2022/04/27 06:00 [medline]
PHST- 2021/12/28 08:55 [entrez]
AID - 10.1002/kjm2.12495 [doi]
PST - ppublish
SO  - Kaohsiung J Med Sci. 2022 May;38(5):457-468. doi: 10.1002/kjm2.12495. Epub 2021 
      Dec 28.