PMID- 34965999
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220716
IS  - 2005-3606 (Print)
IS  - 2005-5447 (Electronic)
IS  - 2005-3606 (Linking)
VI  - 15
IP  - 2
DP  - 2022 May 30
TI  - Human Umbilical Cord Mesenchymal Stem Cells Improve the Necrosis and Osteocyte 
      Apoptosis in Glucocorticoid-Induced Osteonecrosis of the Femoral Head Model 
      through Reducing the Macrophage Polarization.
PG  - 195-202
LID - 10.15283/ijsc21120 [doi]
AB  - BACKGROUND AND OBJECTIVES: Apoptosis is an outstanding determinant of 
      glucocorticoid (GC)-induced osteonecrosis of the femoral head (ONFH). Human 
      umbilical cord mesenchymal stem cells (hUC-MSCs) have been demonstrated to be 
      associated with apoptosis in diseases models. However, the role of hUC-MSCs in 
      GC-induced ONFH via regulating apoptosis still needs further study. METHODS AND 
      RESULTS: In the present study, a GC-induced ONFH model was built in vivo through 
      a consecutive injection with lipopolysaccharide (LPS) and methylprednisolone. The 
      necrosis and apoptosis of the femoral head was evaluated by histological and 
      Terminal-deoxynucleoitidyl Transferase Mediated Nick End Labeling (TUNEL) assay. 
      The level of collagen and TRAP positive cells were determined by Masson and TRAP 
      staining, respectively. M1 macrophage polarization was assessed using 
      immunofluorescence assay. The level of proinflammatory cytokines including tumor 
      necrosis factor (TNF)-alpha, Interleukin (IL)-1beta and IL-6 of femoral head was 
      determined by enzyme-linked immunosorbent assay (ELISA) kits. The protein 
      expression of AKT, mTOR, p-AKT and p-mTOR was detected using western blot assay. 
      The results showed that hUC-MSCs treatment prominently promoted the GC-induced 
      the decrease of the collagen level and the increase of TRAP positive cells. 
      Besides, hUC-MSCs treatment decreased necrosis and apoptosis, macrophage 
      polarization, the level of TNF-alpha, IL-1beta and IL-6, the protein expression of p-AKT 
      and p-mTOR, and the radio of p-AKT to AKT and p-mTOR to mTOR of femoral head in 
      vivo. CONCLUSIONS: Therefore, the present study revealed that hUC-MSCs improved 
      the necrosis and osteocyte apoptosis in GC-induced ONFH model through reducing 
      the macrophage polarization, which was associated with the inhibition of AKT/mTOR 
      signaling pathway.
FAU - Tian, Gang
AU  - Tian G
AD  - Department of Orthopedics, Weihai Central Hospital Affiliated to Qingdao 
      University & Qingdao University, Weihai, China.
FAU - Liu, Chuanjie
AU  - Liu C
AD  - Xinxiang Medical University, Xinxiang, China.
AD  - Weihai Key Laboratory of Autoimmunity & Central Laboratory of Weihai Central 
      Hospital, Weihai, China.
FAU - Gong, Qi
AU  - Gong Q
AD  - Weihai Key Laboratory of Autoimmunity & Central Laboratory of Weihai Central 
      Hospital, Weihai, China.
FAU - Yu, Zhiping
AU  - Yu Z
AD  - Department of Sports Medicine, Weihai Central Hospital Affiliated to Qingdao 
      University, Weihai, China.
FAU - Wang, Haitao
AU  - Wang H
AD  - Department of Trauma Surgery, Weihai Central Hospital Affiliated to Qingdao 
      University, Weihai, China.
FAU - Zhang, Daoqiang
AU  - Zhang D
AD  - Weihai Key Laboratory of Autoimmunity & Central Laboratory of Weihai Central 
      Hospital, Weihai, China.
FAU - Cong, Haibo
AU  - Cong H
AD  - Department of Orthopedics, Weihai Central Hospital Affiliated to Qingdao 
      University & Weihai Key Laboratory of Autoimmunity, Weihai, China.
LA  - eng
PT  - Journal Article
PL  - Korea (South)
TA  - Int J Stem Cells
JT  - International journal of stem cells
JID - 101497587
PMC - PMC9148830
OTO - NOTNLM
OT  - Apoptosis
OT  - Glucocorticoid
OT  - Human umbilical cord mesenchymal stem cells
OT  - Macrophage polarization
OT  - Osteonecrosis of the femoral head (ONFH)
COIS- Potential Conflict of Interest The authors have no conflicting financial 
      interest.
EDAT- 2021/12/31 06:00
MHDA- 2021/12/31 06:01
PMCR- 2021/12/31
CRDT- 2021/12/30 05:27
PHST- 2021/06/28 00:00 [received]
PHST- 2021/10/01 00:00 [revised]
PHST- 2021/10/25 00:00 [accepted]
PHST- 2021/12/31 06:00 [pubmed]
PHST- 2021/12/31 06:01 [medline]
PHST- 2021/12/30 05:27 [entrez]
PHST- 2021/12/31 00:00 [pmc-release]
AID - ijsc21120 [pii]
AID - ijsc-15-2-195 [pii]
AID - 10.15283/ijsc21120 [doi]
PST - ppublish
SO  - Int J Stem Cells. 2022 May 30;15(2):195-202. doi: 10.15283/ijsc21120.