PMID- 34968291
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220101
IS  - 2075-4655 (Electronic)
IS  - 2075-4655 (Linking)
VI  - 4
IP  - 3
DP  - 2020 Sep 1
TI  - Genome-Wide DNA Methylation and LncRNA-Associated DNA Methylation in 
      Metformin-Treated and -Untreated Diabetes.
LID - 10.3390/epigenomes4030019 [doi]
LID - 19
AB  - Metformin, which is used as a first line treatment for type 2 diabetes mellitus 
      (T2DM), has been shown to affect epigenetic patterns. In this study, we 
      investigated the DNA methylation and potential lncRNA modifications in 
      metformin-treated and newly diagnosed adults with T2DM. Genome-wide DNA 
      methylation and lncRNA analysis were performed from the peripheral blood of 12 
      screen-detected and 12 metformin-treated T2DM individuals followed by gene 
      ontology (GO) and KEGG pathway analysis. Differentially methylated regions (DMRs) 
      observed showed 22 hypermethylated and 11 hypomethylated DMRs between individuals 
      on metformin compared to screen-detected subjects. Amongst the hypomethylated DMR 
      regions were the SLC gene family, specifically, SLC25A35 and SLC28A1. Fifty-seven 
      lncRNA-associated DNA methylation regions included the mitochondrial ATP 
      synthase-coupling factor 6 (ATP5J). Functional gene mapping and pathway analysis 
      identified regions in the axon initial segment (AIS), node of Ranvier, cell 
      periphery, cleavage furrow, cell surface furrow, and stress fiber. In conclusion, 
      our study has identified a number of DMRs and lncRNA-associated DNA methylation 
      regions in metformin-treated T2DM that are potential targets for therapeutic 
      monitoring in patients with diabetes.
FAU - Solomon, Wendy L
AU  - Solomon WL
AUID- ORCID: 0000-0003-2960-939X
AD  - SAMRC/CPUT/Cardiometabolic Health Research Unit, Department of Biomedical 
      Sciences, Faculty of Health and Wellness Science, Cape Peninsula University of 
      Technology, P.O. Box 1906, Bellville, Cape Town 7530, South Africa.
FAU - Hector, Stanton B E
AU  - Hector SBE
AD  - SAMRC/CPUT/Cardiometabolic Health Research Unit, Department of Biomedical 
      Sciences, Faculty of Health and Wellness Science, Cape Peninsula University of 
      Technology, P.O. Box 1906, Bellville, Cape Town 7530, South Africa.
FAU - Raghubeer, Shanel
AU  - Raghubeer S
AUID- ORCID: 0000-0002-8161-5458
AD  - SAMRC/CPUT/Cardiometabolic Health Research Unit, Department of Biomedical 
      Sciences, Faculty of Health and Wellness Science, Cape Peninsula University of 
      Technology, P.O. Box 1906, Bellville, Cape Town 7530, South Africa.
FAU - Erasmus, Rajiv T
AU  - Erasmus RT
AD  - Division of Chemical Pathology, Faculty of Health Sciences, National Health 
      Laboratory Service (NHLS) and University of Stellenbosch, Cape Town 7505, South 
      Africa.
FAU - Kengne, Andre P
AU  - Kengne AP
AUID- ORCID: 0000-0002-5183-131X
AD  - Non-Communicable Diseases Research Unit, South African Medical Research Council, 
      Cape Town 7505, South Africa.
AD  - Department of Medicine, University of Cape Town, Cape Town 7700, South Africa.
FAU - Matsha, Tandi E
AU  - Matsha TE
AUID- ORCID: 0000-0001-5251-030X
AD  - SAMRC/CPUT/Cardiometabolic Health Research Unit, Department of Biomedical 
      Sciences, Faculty of Health and Wellness Science, Cape Peninsula University of 
      Technology, P.O. Box 1906, Bellville, Cape Town 7530, South Africa.
LA  - eng
GR  - MRC-RFA-UFSP-01-2013/VMH Study/South African Medical Research Council/
PT  - Journal Article
DEP - 20200901
PL  - Switzerland
TA  - Epigenomes
JT  - Epigenomes
JID - 101736595
PMC - PMC8594715
OTO - NOTNLM
OT  - Africa
OT  - DNA methylation
OT  - diabetes mellitus
OT  - lncRNA
OT  - metformin
COIS- The authors declare no conflict of interests.
EDAT- 2020/09/01 00:00
MHDA- 2020/09/01 00:01
PMCR- 2020/09/01
CRDT- 2021/12/30 17:14
PHST- 2020/07/08 00:00 [received]
PHST- 2020/08/03 00:00 [revised]
PHST- 2020/08/14 00:00 [accepted]
PHST- 2021/12/30 17:14 [entrez]
PHST- 2020/09/01 00:00 [pubmed]
PHST- 2020/09/01 00:01 [medline]
PHST- 2020/09/01 00:00 [pmc-release]
AID - epigenomes4030019 [pii]
AID - epigenomes-04-00019 [pii]
AID - 10.3390/epigenomes4030019 [doi]
PST - epublish
SO  - Epigenomes. 2020 Sep 1;4(3):19. doi: 10.3390/epigenomes4030019.