PMID- 34970699
OWN - NLM
STAT- MEDLINE
DCOM- 20220314
LR  - 20231213
IS  - 1791-2423 (Electronic)
IS  - 1019-6439 (Linking)
VI  - 60
IP  - 1
DP  - 2022 Jan
TI  - Cx32 promotes autophagy and produces resistance to SN‑induced apoptosis via 
      activation of AMPK signalling in cervical cancer.
LID - 10 [pii]
LID - 10.3892/ijo.2021.5300 [doi]
AB  - The roles of gap junctions (GJs) and its components, connexins, in the autophagy 
      of cervical cancer cells have been rarely investigated. Our previous study 
      demonstrated that connexin 32 (Cx32) exerted an anti‑apoptotic effect on cervical 
      cancer. However, as an important regulator of apoptosis, whether the autophagy is 
      involved in the function of Cx32 on cervical cancer cells is not well defined. 
      The present study aimed to investigate the role of Cx32 on autophagy and 
      apoptosis inhibition in cervical cancer cells. The expression levels of Cx32 and 
      the autophagy‑associated protein LC3‑Ⅱ in paracancerous cervical tissues (n=30) 
      and cervical cancer (n=50) tissues were determined via western blotting. In 
      total, 45 cervical cancer specimens were used to evaluate the clinical relevance 
      of Cx32 and LC3‑Ⅱ. It was found that both Cx32 and LC3‑Ⅱ were upregulated in 
      cervical cancer tissues compared with those in paracancerous cervical tissues. 
      The effect of Cx32 on autophagy was examined by detecting the change of LC3‑Ⅱ 
      using western blotting, transfection with enhanced green fluorescent protein‑LC3 
      plasmid and transmission electron microscopy analysis. Overexpression of Cx32 
      significantly enhanced autophagy in HeLa‑Cx32 cells, whereas knockdown of Cx32 
      suppressed autophagy in C‑33A cells. The flow cytometry results demonstrated that 
      Cx32 inhibited the apoptosis of cervical cancer cells by promoting autophagy. 
      Moreover, Cx32 triggered autophagy via the activation of the AMP‑activated 
      protein kinase (AMPK) signalling, regardless of the presence or absence of GJs. 
      Collectively, it was identified that Cx32 exerted its anti‑apoptotic effect by 
      activating autophagy via the AMPK pathway in cervical cancer, which demonstrates 
      a novel mechanism for Cx32 in human cervical cancer progression.
FAU - Fan, Li-Xia
AU  - Fan LX
AD  - Department of Pharmacology, Zhongshan School of Medicine, Sun Yat‑Sen University, 
      Guangzhou, Guangdong 510080, P.R. China.
FAU - Tao, Liang
AU  - Tao L
AD  - Department of Pharmacology, Zhongshan School of Medicine, Sun Yat‑Sen University, 
      Guangzhou, Guangdong 510080, P.R. China.
FAU - Lai, Yong-Chang
AU  - Lai YC
AD  - Department of Urology, The Eighth Affiliated Hospital, Sun Yat‑Sen University, 
      Shenzhen, Guangdong 518033, P.R. China.
FAU - Cai, Shao-Yi
AU  - Cai SY
AD  - Department of Pharmacology, Zhongshan School of Medicine, Sun Yat‑Sen University, 
      Guangzhou, Guangdong 510080, P.R. China.
FAU - Zhao, Zi-Yu
AU  - Zhao ZY
AD  - Department of Pharmacology, Zhongshan School of Medicine, Sun Yat‑Sen University, 
      Guangzhou, Guangdong 510080, P.R. China.
FAU - Yang, Feng
AU  - Yang F
AD  - Department of Pharmacology, Zhongshan School of Medicine, Sun Yat‑Sen University, 
      Guangzhou, Guangdong 510080, P.R. China.
FAU - Su, Ri-Ya
AU  - Su RY
AD  - Department of Pharmacology, Zhongshan School of Medicine, Sun Yat‑Sen University, 
      Guangzhou, Guangdong 510080, P.R. China.
FAU - Wang, Qin
AU  - Wang Q
AD  - Department of Pharmacology, Zhongshan School of Medicine, Sun Yat‑Sen University, 
      Guangzhou, Guangdong 510080, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20211231
PL  - Greece
TA  - Int J Oncol
JT  - International journal of oncology
JID - 9306042
RN  - 0 (Connexins)
RN  - EC 2.7.11.31 (AMP-Activated Protein Kinases)
SB  - IM
MH  - AMP-Activated Protein Kinases/*metabolism
MH  - Autophagy/*genetics/physiology
MH  - Cell Line, Tumor/metabolism
MH  - Connexins/metabolism/*pharmacology
MH  - Female
MH  - Humans
MH  - Signal Transduction/genetics
MH  - Uterine Cervical Neoplasms/*genetics/physiopathology
MH  - Gap Junction beta-1 Protein
OTO - NOTNLM
OT  - AMP‑activated protein kinase
OT  - apoptosis
OT  - autophagy
OT  - cervical carcinoma
OT  - connexin 32
EDAT- 2022/01/01 06:00
MHDA- 2022/03/15 06:00
CRDT- 2021/12/31 06:09
PHST- 2021/06/25 00:00 [received]
PHST- 2021/10/01 00:00 [accepted]
PHST- 2021/12/31 06:09 [entrez]
PHST- 2022/01/01 06:00 [pubmed]
PHST- 2022/03/15 06:00 [medline]
AID - 10 [pii]
AID - 10.3892/ijo.2021.5300 [doi]
PST - ppublish
SO  - Int J Oncol. 2022 Jan;60(1):10. doi: 10.3892/ijo.2021.5300. Epub 2021 Dec 31.