PMID- 34971932
OWN - NLM
STAT- Publisher
LR  - 20231019
IS  - 1876-7753 (Electronic)
IS  - 1873-5061 (Linking)
VI  - 59
DP  - 2022 Mar
TI  - Generation of a B2M homozygous knockout human somatic cell nuclear 
      transfer-derived embryonic stem cell line using the CRISPR/Cas9 system.
PG  - 102643
LID - S1873-5061(21)00490-6 [pii]
LID - 10.1016/j.scr.2021.102643 [doi]
AB  - Beta2-microglobulin (B2M) is a subunit of human leukocyte antigen class-I (HLA-I) 
      heterodimer that mediates immune rejection through activation of cytotoxic T 
      cells. B2M binding to HLA-I proteins is essential for functional HLA-I on the 
      cell surface. Here, we generated a B2M homozygous knockout somatic cell nuclear 
      transfer-induced embryonic stem cell (SCNT-ESC) line using CRISPR/Cas9-mediated 
      gene targeting. B2M KO cell line, which does not express HLA-I molecules on cell 
      surface, has pluripotency and differentiation ability to three germ layers. This 
      cell line provides a useful cell source for investigating immunogenicity of 
      allogeneic ESCs and their derivatives for tissue regeneration.
CI  - Copyright (c) 2021 The Author(s). Published by Elsevier B.V. All rights reserved.
FAU - Lee, Ok-Hee
AU  - Lee OH
AD  - Department of Biomedical Science, CHA University, Seongnam-si, Gyeonggi-do 13488, 
      Republic of Korea; Department of Veterinary Physiology, College of Veterinary 
      Medicine, Konkuk University, Seoul 05029, Republic of Korea.
FAU - Lee, Siyoung
AU  - Lee S
AD  - Department of Stem Cell and Regenerative Biotechnology, Konkuk University, Seoul 
      05029, Republic of Korea.
FAU - Park, Miseon
AU  - Park M
AD  - Fertility Center of CHA Gangnam Medical Center, Department of Obstetrics and 
      Gynecology, CHA University, Seoul 06135, Republic of Korea.
FAU - Moon, Sohyeon
AU  - Moon S
AD  - Department of Stem Cell and Regenerative Biotechnology, Konkuk University, Seoul 
      05029, Republic of Korea.
FAU - Hwang, Semi
AU  - Hwang S
AD  - Department of Stem Cell and Regenerative Biotechnology, Konkuk University, Seoul 
      05029, Republic of Korea.
FAU - Kim, Byeongseok
AU  - Kim B
AD  - Department of Stem Cell and Regenerative Biotechnology, Konkuk University, Seoul 
      05029, Republic of Korea.
FAU - Kim, C-Yoon
AU  - Kim CY
AD  - Department of Veterinary Physiology, College of Veterinary Medicine, Konkuk 
      University, Seoul 05029, Republic of Korea.
FAU - Lee, Dong Ryul
AU  - Lee DR
AD  - Department of Biomedical Science, CHA University, Seongnam-si, Gyeonggi-do 13488, 
      Republic of Korea.
FAU - Shim, Sung Han
AU  - Shim SH
AD  - Department of Biomedical Science, CHA University, Seongnam-si, Gyeonggi-do 13488, 
      Republic of Korea.
FAU - Park, Keun-Hong
AU  - Park KH
AD  - Department of Biomedical Science, CHA University, Seongnam-si, Gyeonggi-do 13488, 
      Republic of Korea.
FAU - Chung, Hyung Min
AU  - Chung HM
AD  - Department of Stem Cell Biology, School of Medicine, Konkuk University, Seoul 
      05029, Republic of Korea.
FAU - Choi, Youngsok
AU  - Choi Y
AD  - Department of Stem Cell and Regenerative Biotechnology, Konkuk University, Seoul 
      05029, Republic of Korea. Electronic address: choiys3969@konkuk.ac.kr.
LA  - eng
PT  - Journal Article
DEP - 20211227
PL  - England
TA  - Stem Cell Res
JT  - Stem cell research
JID - 101316957
SB  - IM
EDAT- 2022/01/01 06:00
MHDA- 2022/01/01 06:00
CRDT- 2021/12/31 20:18
PHST- 2021/10/26 00:00 [received]
PHST- 2021/11/23 00:00 [revised]
PHST- 2021/12/26 00:00 [accepted]
PHST- 2022/01/01 06:00 [pubmed]
PHST- 2022/01/01 06:00 [medline]
PHST- 2021/12/31 20:18 [entrez]
AID - S1873-5061(21)00490-6 [pii]
AID - 10.1016/j.scr.2021.102643 [doi]
PST - ppublish
SO  - Stem Cell Res. 2022 Mar;59:102643. doi: 10.1016/j.scr.2021.102643. Epub 2021 Dec 
      27.