PMID- 34972704
OWN - NLM
STAT- MEDLINE
DCOM- 20220104
LR  - 20220531
IS  - 1791-7549 (Electronic)
IS  - 0258-851X (Print)
IS  - 0258-851X (Linking)
VI  - 36
IP  - 1
DP  - 2022 Jan-Feb
TI  - Telomerase Reverse Transcriptase Promoter Mutations in Human Hepatobiliary, 
      Pancreatic and Gastrointestinal Cancer Cell Lines.
PG  - 94-102
LID - 10.21873/invivo.12680 [doi]
AB  - BACKGROUND/AIM: The promoter region of the telomerase reverse transcriptase 
      (TERT) gene is a regulatory element capable of affecting TERT expression, 
      telomerase activity, and telomerase length. Mutations within the TERT promoter 
      region are the most common mutations in many cancers. In this study, we 
      characterized the TERT promoter mutation status in hepatobiliary, pancreatic, and 
      gastrointestinal cancer cell lines. MATERIALS AND METHODS: TERT promoter mutation 
      status was assessed by digital PCR in 12 liver cancer, 5 cholangiocarcinoma 
      (CCA), 12 pancreatic cancer, 17 gastrointestinal cancer, and 3 healthy control 
      cell lines. RESULTS: The C228T promoter mutation was detected in 9 liver cancer 
      lines, and the C250T TERT mutation was detected in 1 oesophageal squamous cell 
      carcinoma line. CONCLUSION: The C228T promoter mutation is specific to liver 
      cancer cell lines among various gastrointestinal cancer cell lines. These data 
      will contribute to future research on the tumorigenic mechanisms and clinical use 
      of digital PCR to detect mutations.
CI  - Copyright (c) 2022 International Institute of Anticancer Research (Dr. George J. 
      Delinasios), All rights reserved.
FAU - Hirata, Masahiro
AU  - Hirata M
AD  - Department of Gastroenterology and Neurology, Kagawa University, Kagawa, Japan.
FAU - Fujita, Koji
AU  - Fujita K
AD  - Department of Gastroenterology and Neurology, Kagawa University, Kagawa, Japan.
FAU - Fujihara, Shintaro
AU  - Fujihara S
AD  - Department of Gastroenterology and Neurology, Kagawa University, Kagawa, Japan.
FAU - Mizuo, Takaaki
AU  - Mizuo T
AD  - Department of Gastroenterology and Neurology, Kagawa University, Kagawa, Japan.
FAU - Nakabayashi, Ryota
AU  - Nakabayashi R
AD  - Department of Gastroenterology and Neurology, Kagawa University, Kagawa, Japan.
FAU - Kono, Toshiaki
AU  - Kono T
AD  - Department of Gastroenterology and Neurology, Kagawa University, Kagawa, Japan.
FAU - Namima, Daisuke
AU  - Namima D
AD  - Department of Gastroenterology and Neurology, Kagawa University, Kagawa, Japan.
FAU - Fujita, Naoki
AU  - Fujita N
AD  - Department of Gastroenterology and Neurology, Kagawa University, Kagawa, Japan.
FAU - Yamana, Hiroki
AU  - Yamana H
AD  - Department of Gastroenterology and Neurology, Kagawa University, Kagawa, Japan.
FAU - Kamada, Hideki
AU  - Kamada H
AD  - Department of Gastroenterology and Neurology, Kagawa University, Kagawa, Japan.
FAU - Tani, Joji
AU  - Tani J
AD  - Department of Gastroenterology and Neurology, Kagawa University, Kagawa, Japan.
FAU - Kobara, Hideki
AU  - Kobara H
AD  - Department of Gastroenterology and Neurology, Kagawa University, Kagawa, Japan.
FAU - Tsutsui, Kunihiko
AU  - Tsutsui K
AD  - Department of Gastroenterology and Neurology, Kagawa University, Kagawa, Japan.
FAU - Matsuda, Yoko
AU  - Matsuda Y
AD  - Department of Pathology and Host-Defense, Faculty of Medicine, Kagawa University, 
      Kagawa, Japan.
FAU - Ono, Masafumi
AU  - Ono M
AD  - Department of Gastroenterology and Neurology, Kagawa University, Kagawa, Japan.
FAU - Masaki, Tsutomu
AU  - Masaki T
AD  - Department of Gastroenterology and Neurology, Kagawa University, Kagawa, Japan; 
      tmasaki@med.kagawa-u.ac.jp.
LA  - eng
PT  - Journal Article
PL  - Greece
TA  - In Vivo
JT  - In vivo (Athens, Greece)
JID - 8806809
RN  - EC 2.7.7.49 (TERT protein, human)
RN  - EC 2.7.7.49 (Telomerase)
SB  - IM
MH  - Cell Line
MH  - *Gastrointestinal Neoplasms/genetics
MH  - Humans
MH  - *Liver Neoplasms/genetics
MH  - Mutation
MH  - Promoter Regions, Genetic
MH  - *Telomerase/genetics/metabolism
PMC - PMC8765179
OTO - NOTNLM
OT  - TERT
OT  - TERT promoter C228T
OT  - TERT promoter C250T
OT  - cancer
OT  - digital PCR
OT  - gastrointestinal
OT  - hepatobiliary
OT  - telomerase
COIS- The Authors declare that they have no competing interests in relation to this 
      study.
EDAT- 2022/01/02 06:00
MHDA- 2022/01/05 06:00
PMCR- 2022/01/03
CRDT- 2022/01/01 05:18
PHST- 2021/10/05 00:00 [received]
PHST- 2021/09/27 00:00 [revised]
PHST- 2021/10/29 00:00 [accepted]
PHST- 2022/01/01 05:18 [entrez]
PHST- 2022/01/02 06:00 [pubmed]
PHST- 2022/01/05 06:00 [medline]
PHST- 2022/01/03 00:00 [pmc-release]
AID - 36/1/94 [pii]
AID - 10.21873/invivo.12680 [doi]
PST - ppublish
SO  - In Vivo. 2022 Jan-Feb;36(1):94-102. doi: 10.21873/invivo.12680.