PMID- 34973620
OWN - NLM
STAT- MEDLINE
DCOM- 20220331
LR  - 20220401
IS  - 1879-0054 (Electronic)
IS  - 0739-7240 (Linking)
VI  - 79
DP  - 2022 Apr
TI  - Dietary carbohydrate influences the colocalization pattern of Glucagon-like 
      Peptide-1 with neurotensin in the chicken ileum.
PG  - 106693
LID - S0739-7240(21)00089-8 [pii]
LID - 10.1016/j.domaniend.2021.106693 [doi]
AB  - Glucagon-like peptide (GLP)-1 colocalizes with neurotensin (NT) in the same 
      enteroendocrine cells (EECs) of the chicken ileum. The present study was designed 
      to clarify the influence of dietary carbohydrate (CHO) on the colocalization 
      pattern of GLP-1 with NT in the chicken distal ileum. Male White Leghorn chickens 
      at 6 weeks of age (n = 15) were divided into three groups, a control and two 
      experimental (low-CHO and CHO-free), with five chickens in each, and fed control 
      or experimental diets for 7 d. Distal ileum was collected from each bird as a 
      tissue sample and subjected to double immunofluorescence staining to detect GLP-1 
      and NT. Three types of EEC, GLP-1+/NT+, GLP-1+/NT- and GLP-1-/NT+, were 
      demonstrated in the chicken ileum. GLP-1+/NT+ cells in the control group had a 
      spindle-like shape with a long cytoplasmic process, but those in the experimental 
      groups were round and lacked a cytoplasmic process. The ratio of GLP-1+/NT+ cells 
      was significantly decreased in the two experimental groups compared with that in 
      the control group. The ratio of GLP-1+/NT+ cells was significantly lower than 
      those of GLP-1+/NT- and GLP-1-/NT+ cells in the two experimental groups. Most 
      cells that were immunoreactive for GLP-1 and NT antisera lacked signals of 
      proglucagon (PG) and NT precursor (NTP) mRNA in the experimental groups. The 
      number of EECs expressing PG and NTP mRNA signals showed tendencies for decreases 
      with a reduction of dietary CHO level. These findings suggest that dietary CHO 
      could be a significant regulator of the pattern of colocalization pattern of 
      GLP-1 with NT in the chicken ileum.
CI  - Copyright (c) 2021 Elsevier Inc. All rights reserved.
FAU - Salahuddin, M
AU  - Salahuddin M
AD  - Department of Science and Technology, Graduate School of Medicine, Science and 
      Technology, Shinshu University, Kami-ina, Nagano 399-4598, Japan.
FAU - Hiramatsu, K
AU  - Hiramatsu K
AD  - Laboratory of Animal Functional Anatomy (LAFA), Faculty of Agriculture, Shinshu 
      University, Kami-ina, Nagano 399-4598, Japan. Electronic address: 
      seitaik@shinshu-u.ac.jp.
FAU - Kita, K
AU  - Kita K
AD  - Laboratory of Animal Nutrition, Faculty of Agriculture, Iwate University, 
      Morioka, Iwate 020-8550, Japan.
LA  - eng
PT  - Journal Article
DEP - 20211119
PL  - United States
TA  - Domest Anim Endocrinol
JT  - Domestic animal endocrinology
JID - 8505191
RN  - 0 (Dietary Carbohydrates)
RN  - 0 (Glucagon-Like Peptide 2)
RN  - 39379-15-2 (Neurotensin)
RN  - 89750-14-1 (Glucagon-Like Peptide 1)
SB  - IM
MH  - Animals
MH  - Chickens
MH  - Dietary Carbohydrates
MH  - *Glucagon-Like Peptide 1/genetics
MH  - Glucagon-Like Peptide 2
MH  - Ileum
MH  - Male
MH  - *Neurotensin
OTO - NOTNLM
OT  - Carbohydrate
OT  - Chicken
OT  - Colocalization
OT  - Glucagon-like peptide 1
OT  - Ileum
OT  - Neurotensin
EDAT- 2022/01/02 06:00
MHDA- 2022/04/01 06:00
CRDT- 2022/01/01 20:21
PHST- 2021/10/11 00:00 [received]
PHST- 2021/11/14 00:00 [revised]
PHST- 2021/11/15 00:00 [accepted]
PHST- 2022/01/02 06:00 [pubmed]
PHST- 2022/04/01 06:00 [medline]
PHST- 2022/01/01 20:21 [entrez]
AID - S0739-7240(21)00089-8 [pii]
AID - 10.1016/j.domaniend.2021.106693 [doi]
PST - ppublish
SO  - Domest Anim Endocrinol. 2022 Apr;79:106693. doi: 10.1016/j.domaniend.2021.106693. 
      Epub 2021 Nov 19.