PMID- 34973969 OWN - NLM STAT- MEDLINE DCOM- 20220317 LR - 20220317 IS - 1095-6840 (Electronic) IS - 0016-6480 (Linking) VI - 317 DP - 2022 Feb 1 TI - Distribution of kisspeptin system and its relation with gonadotropin-releasing hormone in the hypothalamus of the South American plains vizcacha, Lagostomus maximus. PG - 113974 LID - S0016-6480(21)00267-7 [pii] LID - 10.1016/j.ygcen.2021.113974 [doi] AB - Kisspeptin (KISS), a key hormone involved in the regulation of the hypothalamic-pituitary-ovarian (HPO) axis, has been localized in the anteroventral periventricular (AVPV) nucleus and the neighboring rostral periventricular nucleus (PeVN), and in the arcuate (ARC) nucleus of the mammalian hypothalamus. In the ARC, the KISS neurons that co-express neurokinin B (NKB) and dynorphin A (Dyn) are named KNDy cells. The South American plains vizcacha is a rodent with peculiar reproductive traits. Around mid-pregnancy, vizcacha shows the reactivation of its HPO axis with the pulsatile release of gonadotropin-releasing hormone (GnRH) and luteinizing hormone (LH), an essential event for the success of gestation. Considering the role of KISS system in GnRH modulation, the aim of this work was to study their neuroanatomical distribution in adult vizcachas. AVPV showed sexual dimorphism with a significant smaller area in males (t-Test, p < 0.05), and KISS immunoreactivity was detected in somas and varicosities homogenously distributed in the AVPV with a concordant sex-related expression pattern. NKB and Dyn expression was also observed in cytoplasm of neurons scattered in the AVPV. Three subpopulations of neurons were detected in the AVPV: neurons expressing Dyn and NKB (DyNK cells), neurons expressing KISS and NKB (KiNK cells), and single NKB expressing neurons. Strikingly, KISS and Dyn were always expressed in different cells. In addition, in the ARC nucleus, KNDy cells were detected. On the other hand, KISS and GnRH expression was detected in different subpopulations of neurons, GnRH cells showed KISS receptor (KISSR or GPR-54) expression, and KISS immunoreactive afferent contacts were detected making close appositions onto somas and dendrites of GnRH cells. These results show similarities and differences between the KISS system in the hypothalamus of the vizcacha and other mammals, and constitute crucial observations about KISS and GnRH relation. Considering the peculiarity of HPO axis regulation in this species, the present work provides a neuroanatomical framework for the further elucidation of molecular mechanisms underlying GnRH expression and secretion. CI - Copyright (c) 2021 Elsevier Inc. All rights reserved. FAU - Raul Schmidt, Alejandro AU - Raul Schmidt A AD - Centro de Estudios Biomedicos Basicos, Aplicados y Desarrollo (CEBBAD), Universidad Maimonides, Ciudad Autonoma de Buenos Aires, Argentina; Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET), Argentina. FAU - Ignacio Felipe Inserra, Pablo AU - Ignacio Felipe Inserra P AD - Centro de Estudios Biomedicos Basicos, Aplicados y Desarrollo (CEBBAD), Universidad Maimonides, Ciudad Autonoma de Buenos Aires, Argentina; Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET), Argentina. FAU - Andres Cortasa, Santiago AU - Andres Cortasa S AD - Centro de Estudios Biomedicos Basicos, Aplicados y Desarrollo (CEBBAD), Universidad Maimonides, Ciudad Autonoma de Buenos Aires, Argentina; Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET), Argentina. FAU - Proietto, Sofia AU - Proietto S AD - Centro de Estudios Biomedicos Basicos, Aplicados y Desarrollo (CEBBAD), Universidad Maimonides, Ciudad Autonoma de Buenos Aires, Argentina; Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET), Argentina. FAU - Fidel, Victoria AU - Fidel V AD - Centro de Estudios Biomedicos Basicos, Aplicados y Desarrollo (CEBBAD), Universidad Maimonides, Ciudad Autonoma de Buenos Aires, Argentina. FAU - Halperin, Julia AU - Halperin J AD - Centro de Estudios Biomedicos Basicos, Aplicados y Desarrollo (CEBBAD), Universidad Maimonides, Ciudad Autonoma de Buenos Aires, Argentina; Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET), Argentina. FAU - Daniel Vitullo, Alfredo AU - Daniel Vitullo A AD - Centro de Estudios Biomedicos Basicos, Aplicados y Desarrollo (CEBBAD), Universidad Maimonides, Ciudad Autonoma de Buenos Aires, Argentina; Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET), Argentina. FAU - Berta Dorfman, Veronica AU - Berta Dorfman V AD - Centro de Estudios Biomedicos Basicos, Aplicados y Desarrollo (CEBBAD), Universidad Maimonides, Ciudad Autonoma de Buenos Aires, Argentina; Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET), Argentina. Electronic address: dorfman.veronica@maimonides.edu. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20211230 PL - United States TA - Gen Comp Endocrinol JT - General and comparative endocrinology JID - 0370735 RN - 0 (Kisspeptins) RN - 33515-09-2 (Gonadotropin-Releasing Hormone) RN - 86933-75-7 (Neurokinin B) SB - IM MH - Animals MH - Arcuate Nucleus of Hypothalamus/metabolism MH - Female MH - *Gonadotropin-Releasing Hormone/metabolism MH - Hypothalamus/metabolism MH - *Kisspeptins/metabolism MH - Male MH - Mammals/metabolism MH - Neurokinin B/metabolism MH - Pregnancy MH - South America OTO - NOTNLM OT - Dynorphin A OT - GnRH OT - Hypothalamus OT - Kisspeptin OT - Neurokinin B OT - Vizcacha EDAT- 2022/01/03 06:00 MHDA- 2022/03/18 06:00 CRDT- 2022/01/02 20:21 PHST- 2021/04/01 00:00 [received] PHST- 2021/12/21 00:00 [revised] PHST- 2021/12/26 00:00 [accepted] PHST- 2022/01/03 06:00 [pubmed] PHST- 2022/03/18 06:00 [medline] PHST- 2022/01/02 20:21 [entrez] AID - S0016-6480(21)00267-7 [pii] AID - 10.1016/j.ygcen.2021.113974 [doi] PST - ppublish SO - Gen Comp Endocrinol. 2022 Feb 1;317:113974. doi: 10.1016/j.ygcen.2021.113974. Epub 2021 Dec 30.