PMID- 34975072
OWN - NLM
STAT- MEDLINE
DCOM- 20220830
LR  - 20220924
IS  - 1745-7262 (Electronic)
IS  - 1008-682X (Print)
IS  - 1008-682X (Linking)
VI  - 24
IP  - 5
DP  - 2022 Sep-Oct
TI  - MACS-annexin V cell sorting of semen samples with high TUNEL values decreases the 
      concentration of cells with abnormal chromosomal content: a pilot study.
PG  - 445-450
LID - 10.4103/aja202197 [doi]
AB  - We question whether, in men with an abnormal rate of sperm DNA fragmentation, the 
      magnetic-activated cell sorting (MACS) could select spermatozoa with lower rates 
      of DNA fragmentation as well as spermatozoa with unbalanced chromosome content. 
      Cryopreserved spermatozoa from six males were separated into nonapoptotic and 
      apoptotic populations. We determined the percentages of spermatozoa with (i) 
      externalization of phosphatidylserine (EPS) by annexin V-Fluorescein 
      isothiocyanate (FITC) labeling, (ii) DNA fragmentation by TdT-mediated-dUTP 
      nick-end labeling (TUNEL), and (iii) numerical abnormalities for chromosomes X, 
      Y, 13, 18, and 21 by fluorescence in situ hybridization (FISH), on the whole 
      ejaculate and selected spermatozoa in the same patient. Compared to the 
      nonapoptotic fraction, the apoptotic fraction statistically showed a higher 
      number of spermatozoa with EPS, with DNA fragmentation, and with numerical 
      chromosomal abnormalities. Compared to the whole ejaculate, we found a 
      significant decrease in the percentage of spermatozoa with EPS and decrease 
      tendencies of the DNA fragmentation rate and the sum of disomy levels in the 
      nonapoptotic fraction. Conversely, we observed statistically significant higher 
      rates of these three parameters in the apoptotic fraction. MACS may help to 
      select spermatozoa with lower rates of DNA fragmentation and unbalanced 
      chromosome content in men with abnormal rates of sperm DNA fragmentation.
FAU - El Fekih, Sahar
AU  - El Fekih S
AD  - University of Brest, Inserm, UMR U1078, Faculty of Medicine and Health Sciences, 
      Brest 29238, France.
AD  - Laboratory of Human Cytogenetics, Molecular Genetics and Reproductive Biology, 
      Farhat Hached University Hospital, Sousse 4031, Tunisia.
FAU - Gueganic, Nadia
AU  - Gueganic N
AD  - University of Brest, Inserm, UMR U1078, Faculty of Medicine and Health Sciences, 
      Brest 29238, France.
FAU - Tous, Corinne
AU  - Tous C
AD  - Departement of Medical Genetics and Reproductive Biology, Brest University 
      Regional Hospital, Brest 29609, France.
FAU - Ali, Habib Ben
AU  - Ali HB
AD  - Laboratory of Human Cytogenetics, Molecular Genetics and Reproductive Biology, 
      Farhat Hached University Hospital, Sousse 4031, Tunisia.
FAU - Ajina, Mounir
AU  - Ajina M
AD  - Department of Reproductive Medicine, Farhat Hached Teaching Hospital, Sousse 
      4031, Tunisia.
FAU - Douet-Guilbert, Nathalie
AU  - Douet-Guilbert N
AD  - University of Brest, Inserm, UMR U1078, Faculty of Medicine and Health Sciences, 
      Brest 29238, France.
AD  - Departement of Medical Genetics and Reproductive Biology, Brest University 
      Regional Hospital, Brest 29609, France.
FAU - Drapier, Hortense
AU  - Drapier H
AD  - Departement of Medical Genetics and Reproductive Biology, Brest University 
      Regional Hospital, Brest 29609, France.
FAU - Beauvillard, Damien
AU  - Beauvillard D
AD  - Departement of Medical Genetics and Reproductive Biology, Brest University 
      Regional Hospital, Brest 29609, France.
FAU - Morel, Frederic
AU  - Morel F
AD  - University of Brest, Inserm, UMR U1078, Faculty of Medicine and Health Sciences, 
      Brest 29238, France.
AD  - Departement of Medical Genetics and Reproductive Biology, Brest University 
      Regional Hospital, Brest 29609, France.
FAU - Perrin, Aurore
AU  - Perrin A
AD  - University of Brest, Inserm, UMR U1078, Faculty of Medicine and Health Sciences, 
      Brest 29238, France.
AD  - Departement of Medical Genetics and Reproductive Biology, Brest University 
      Regional Hospital, Brest 29609, France.
LA  - eng
PT  - Journal Article
PL  - China
TA  - Asian J Androl
JT  - Asian journal of andrology
JID - 100942132
RN  - 0 (Annexin A5)
SB  - IM
MH  - Annexin A5
MH  - Chromosome Aberrations
MH  - DNA Fragmentation
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - In Situ Nick-End Labeling
MH  - Male
MH  - Pilot Projects
MH  - *Semen
MH  - *Spermatozoa
PMC - PMC9491034
OTO - NOTNLM
OT  - DNA fragmentation
OT  - chromosomal abnormalities
OT  - externalization of phosphatidylserine
OT  - magnetic-activated cell separation
OT  - spermatozoa
COIS- None
EDAT- 2022/01/04 06:00
MHDA- 2022/08/31 06:00
PMCR- 2021/12/31
CRDT- 2022/01/03 05:30
PHST- 2022/01/04 06:00 [pubmed]
PHST- 2022/08/31 06:00 [medline]
PHST- 2022/01/03 05:30 [entrez]
PHST- 2021/12/31 00:00 [pmc-release]
AID - 334656 [pii]
AID - AJA-24-445 [pii]
AID - 10.4103/aja202197 [doi]
PST - ppublish
SO  - Asian J Androl. 2022 Sep-Oct;24(5):445-450. doi: 10.4103/aja202197.