PMID- 34975821
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220104
IS  - 1664-302X (Print)
IS  - 1664-302X (Electronic)
IS  - 1664-302X (Linking)
VI  - 12
DP  - 2021
TI  - Characterization of Streptococcus salivarius as New Probiotics Derived From Human 
      Breast Milk and Their Potential on Proliferative Inhibition of Liver and Breast 
      Cancer Cells and Antioxidant Activity.
PG  - 797445
LID - 10.3389/fmicb.2021.797445 [doi]
LID - 797445
AB  - Breast milk is well known as the abundant source of beneficial bacteria. A new 
      alternative source of human probiotic origin from breast milk is in demand and 
      currently of interest for both the functional food industry and 
      biopharmaceuticals. The aim in this study was to investigate the anticancer and 
      antioxidant efficacies of the new potential probiotics isolated from human breast 
      milk. Three strains of lactic acid bacteria (LAB) have shown their potential 
      probiotic criteria including antimicrobial activity, non-hemolytic property, and 
      survival in acid and bile salt conditions. These strains showed high abilities on 
      cell surface hydrophobicity, auto-aggregation, and co-aggregation. The genera 
      identification by 16S rRNA sequencing and comparison revealed that they were 
      Streptococcus salivarius BP8, S. salivarius BP156, and S. salivarius BP160. The 
      inhibition of liver cancer cells (HepG2) and breast cancer cells (MCF-7) 
      proliferation by these probiotic strains using a 
      3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was 
      44.83-59.65 and 29.85-37.16%, respectively. The probiotic action mode was 
      inducted via apoptotic mechanisms since they stimulate the liver and breast 
      cancer cell death through DNA fragmentation and positive morphological changes by 
      acridine orange (AO) and propidium iodide (PI) staining. The antioxidant activity 
      of these probiotics in the form of intact cells, cell free supernatant (CFS), and 
      heat-killed cells was evaluated by a 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay, 
      resulting in the scavenging activity rates of 16.93-25.43, 15.47-28.03, and 
      13.67-23.0%, respectively. These S. salivarius probiotic strains protected the 
      L929 mouse fibroblasts against oxidative stress with very high survival rates at 
      94.04-97.77%, which was significantly higher (P < 0.05) than L-ascorbic acid at 
      75.89-78.67% in the control groups. The results indicated that S. salivarius BP8 
      and S. salivarius BP160 probiotic strains could be applied as functional foods or 
      new alternative bioprophylactics for treating liver and breast cancers.
CI  - Copyright (c) 2021 Srikham, Daengprok, Niamsup and Thirabunyanon.
FAU - Srikham, Kantapich
AU  - Srikham K
AD  - Program in Biotechnology, Faculty of Science, Maejo University, Chiang Mai, 
      Thailand.
FAU - Daengprok, Wichittra
AU  - Daengprok W
AD  - Program in Food Science and Technology, Faculty of Engineering and Agro Industry, 
      Maejo University, Chiang Mai, Thailand.
FAU - Niamsup, Piyanuch
AU  - Niamsup P
AD  - Program in Biotechnology, Faculty of Science, Maejo University, Chiang Mai, 
      Thailand.
FAU - Thirabunyanon, Mongkol
AU  - Thirabunyanon M
AD  - Program in Biotechnology, Faculty of Science, Maejo University, Chiang Mai, 
      Thailand.
LA  - eng
PT  - Journal Article
DEP - 20211215
PL  - Switzerland
TA  - Front Microbiol
JT  - Frontiers in microbiology
JID - 101548977
PMC - PMC8714912
OTO - NOTNLM
OT  - anticancer
OT  - antioxidant
OT  - breast milk
OT  - cancer
OT  - lactic acid bacteria (LAB)
OT  - probiotics
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2022/01/04 06:00
MHDA- 2022/01/04 06:01
PMCR- 2021/12/15
CRDT- 2022/01/03 05:37
PHST- 2021/10/18 00:00 [received]
PHST- 2021/11/22 00:00 [accepted]
PHST- 2022/01/03 05:37 [entrez]
PHST- 2022/01/04 06:00 [pubmed]
PHST- 2022/01/04 06:01 [medline]
PHST- 2021/12/15 00:00 [pmc-release]
AID - 10.3389/fmicb.2021.797445 [doi]
PST - epublish
SO  - Front Microbiol. 2021 Dec 15;12:797445. doi: 10.3389/fmicb.2021.797445. 
      eCollection 2021.