PMID- 3497745
OWN - NLM
STAT- MEDLINE
DCOM- 19870928
LR  - 20190820
IS  - 0090-1229 (Print)
IS  - 0090-1229 (Linking)
VI  - 44
IP  - 3
DP  - 1987 Sep
TI  - LPS augments human B-cell differentiation by direct stimulation of PWM-responsive 
      B cells.
PG  - 259-71
AB  - Bacterial lipopolysaccharide (LPS) augments production of IgM and IgG by two- to 
      seven-fold in cultures of peripheral blood lymphocytes (PBL) stimulated by 
      pokeweed mitogen (PWM), but only if monocytes are rigorously depleted. When PBL 
      were separated into adherent cell (AC), B-cell-enriched, and T-cell-enriched 
      fractions, pulsed with LPS, and recombined in culture with PWM, increased 
      generation of plasma cells was seen only in cultures containing LPS-treated B 
      cells. This effect of LPS appears to be independent of soluble factors. 
      Supernatants from LPS-stimulated B cells or AC did not consistently increase PWM 
      responses when cultured with fresh B cells in the presence of polymyxin B. 
      Furthermore, pulsing of B cells with purified interleukin 1 from two different 
      commercial sources failed to augment PWM-induced differentiation. When B cells 
      were depleted of surface IgD (sIgD)-bearing cells by panning, no effect on 
      LPS-mediated augmentation of PWM-driven differentiation was seen. B cells were 
      also fractionated by rosetting with mouse erythrocytes. Treatment of BMR+ cells 
      with LPS did not induce them to respond to PWM, while treatment of BMR- cells 
      with LPS augmented generation of plasma cells. These results indicate that LPS 
      acts directly to augment differentiation of PWM-responsive B cells, rather than 
      recruiting sIgD+, BMR+ cells to become PWM responsive.
FAU - Anderson, S J
AU  - Anderson SJ
FAU - Lawton, A R
AU  - Lawton AR
LA  - eng
GR  - AI-17996/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Clin Immunol Immunopathol
JT  - Clinical immunology and immunopathology
JID - 0356637
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Pokeweed Mitogens)
RN  - 0 (Receptors, Antigen, B-Cell)
SB  - IM
MH  - Adult
MH  - Antibody-Producing Cells/drug effects
MH  - B-Lymphocytes/*classification/cytology/drug effects
MH  - Cell Differentiation/drug effects
MH  - Cell Separation
MH  - Cells, Cultured
MH  - Humans
MH  - Lipopolysaccharides/*pharmacology
MH  - Lymphocyte Activation/*drug effects
MH  - Plasma Cells/drug effects
MH  - Pokeweed Mitogens/pharmacology
MH  - Receptors, Antigen, B-Cell/analysis
MH  - Stimulation, Chemical
EDAT- 1987/09/01 00:00
MHDA- 1987/09/01 00:01
CRDT- 1987/09/01 00:00
PHST- 1987/09/01 00:00 [pubmed]
PHST- 1987/09/01 00:01 [medline]
PHST- 1987/09/01 00:00 [entrez]
AID - 10.1016/0090-1229(87)90070-5 [doi]
PST - ppublish
SO  - Clin Immunol Immunopathol. 1987 Sep;44(3):259-71. doi: 
      10.1016/0090-1229(87)90070-5.