PMID- 3497797
OWN - NLM
STAT- MEDLINE
DCOM- 19870928
LR  - 20071114
IS  - 0013-7227 (Print)
IS  - 0013-7227 (Linking)
VI  - 121
IP  - 3
DP  - 1987 Sep
TI  - Comparison of the bone-resorbing activity in the supernatants from 
      phytohemagglutinin-stimulated human peripheral blood mononuclear cells with that 
      of cytokines through the use of an antiserum to interleukin 1.
PG  - 1164-70
AB  - We compared the bone-resorbing activity in the conditioned medium from 
      phytohemagglutinin (PHA)-stimulated human peripheral blood mononuclear cell 
      cultures with that of partially purified human monocyte-derived interleukin 1 
      (IL-1), human recombinant IL-1 alpha (pI 5) and IL-1 beta (pI 7), human 
      recombinant tumor necrosis factor-alpha (TNF alpha), and PTH in fetal rat long 
      bone cultures. An antiserum to the products of activated human mononuclear cells, 
      including IL-1, completely blocked the bone-resorbing activity of all three forms 
      of IL-1 and of unfractionated PHA-stimulated human peripheral blood mononuclear 
      cell supernatants, but did not inhibit resorption stimulated by recombinant human 
      TNF alpha. This antiserum also had no effect on the resorptive response to 3 nM 
      PTH, but did decrease the response to 1 nM PTH slightly. These results imply that 
      IL-1, but not TNF alpha, mediates the bone-resorbing activity found in the 
      supernatants of PHA-stimulated human peripheral blood mononuclear cell cultures. 
      It is not known whether the small inhibitory effect that the antiserum to IL-1 
      had on the response to 1 nM PTH resulted from a nonspecific action or an effect 
      of PTH on local IL-1 synthesis in bone. Since cytokines are found in the 
      circulation of normal individuals and are produced at local sites of pathology, 
      these results suggest that they can influence both normal and abnormal skeletal 
      metabolism.
FAU - Lorenzo, J A
AU  - Lorenzo JA
FAU - Sousa, S L
AU  - Sousa SL
FAU - Alander, C
AU  - Alander C
FAU - Raisz, L G
AU  - Raisz LG
FAU - Dinarello, C A
AU  - Dinarello CA
LA  - eng
GR  - AI-15614/AI/NIAID NIH HHS/United States
GR  - AR-18063/AR/NIAMS NIH HHS/United States
GR  - AR-31263/AR/NIAMS NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Endocrinology
JT  - Endocrinology
JID - 0375040
RN  - 0 (Glycoproteins)
RN  - 0 (Immune Sera)
RN  - 0 (Interleukin-1)
RN  - 0 (Parathyroid Hormone)
RN  - 0 (Phytohemagglutinins)
RN  - 0 (Prostaglandins E)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Animals
MH  - Bone Resorption/*drug effects
MH  - Bone and Bones/drug effects/metabolism
MH  - Culture Techniques
MH  - Glycoproteins/*pharmacology
MH  - Humans
MH  - Immune Sera/pharmacology
MH  - Interleukin-1/immunology/*physiology
MH  - Monocytes/*physiology
MH  - Parathyroid Hormone/*pharmacology
MH  - Phytohemagglutinins/pharmacology
MH  - Prostaglandins E/metabolism
MH  - Rats
MH  - Recombinant Proteins/pharmacology
MH  - Tumor Necrosis Factor-alpha
EDAT- 1987/09/01 00:00
MHDA- 1987/09/01 00:01
CRDT- 1987/09/01 00:00
PHST- 1987/09/01 00:00 [pubmed]
PHST- 1987/09/01 00:01 [medline]
PHST- 1987/09/01 00:00 [entrez]
AID - 10.1210/endo-121-3-1164 [doi]
PST - ppublish
SO  - Endocrinology. 1987 Sep;121(3):1164-70. doi: 10.1210/endo-121-3-1164.