PMID- 34980756
OWN - NLM
STAT- MEDLINE
DCOM- 20220302
LR  - 20220326
IS  - 1347-7439 (Electronic)
IS  - 0916-7250 (Print)
IS  - 0916-7250 (Linking)
VI  - 84
IP  - 2
DP  - 2022 Mar 1
TI  - Dietary carbohydrate modifies the density of L cells in the chicken ileum.
PG  - 265-274
LID - 10.1292/jvms.21-0572 [doi]
AB  - Glucagon-like peptides (GLPs) are secreted from intestinal L cells and stimulate 
      various physiological functions in the gastrointestinal tract. The secretion of 
      GLPs is influenced by macronutrient ingestion. This study aims to clarify the 
      effects of dietary carbohydrate (CHO) on L cells in the chicken ileum. 
      Six-week-old, male White Leghorn chickens were divided into three groups: 
      control, low-CHO and CHO-free, with five chickens in each group. Paraffin 
      sections were made from the proximal and distal ileum of each animal and 
      subjected to immunohistochemistry for GLP-1 and GLP-2 peptides and in situ 
      hybridization for proglucagon (PG) mRNA. A significant reduction of GLP-1- and 
      GLP-2-immunoreactive cells was observed in the two experimental groups compared 
      with that in the control. A reduction of cells expressing PG mRNA was observed in 
      the proximal and distal ileum of the CHO-free group compared with that in the 
      control. The ratio of GLP-1-immunoreactive cells showing Ki-67 immunoreactivity 
      was significantly lower in the distal ileum of the CHO-free group than that in 
      the control group. These data suggest that dietary CHO is an effective stimulator 
      for modifying L cell density in the chicken ileum.
FAU - Salahuddin, Md
AU  - Salahuddin M
AD  - Department of Science and Technology, Graduate School of Medicine, Science and 
      Technology, Shinshu University, Kami-ina, Nagano 399-4598, Japan.
FAU - Hiramatsu, Kohzy
AU  - Hiramatsu K
AD  - Laboratory of Animal Functional Anatomy (LAFA), Faculty of Agriculture, Shinshu 
      University, Kami-ina, Nagano 399-4598, Japan.
FAU - Nishimoto, Iori
AU  - Nishimoto I
AD  - Laboratory of Animal Functional Anatomy (LAFA), Faculty of Agriculture, Shinshu 
      University, Kami-ina, Nagano 399-4598, Japan.
FAU - Kita, Kazumi
AU  - Kita K
AD  - Laboratory of Animal Nutrition, Faculty of Agriculture, Iwate University, 
      Morioka, Iwate 020-8550, Japan.
LA  - eng
PT  - Journal Article
DEP - 20211229
PL  - Japan
TA  - J Vet Med Sci
JT  - The Journal of veterinary medical science
JID - 9105360
RN  - 0 (Dietary Carbohydrates)
SB  - IM
MH  - Animals
MH  - *Chickens
MH  - *Dietary Carbohydrates/pharmacology
MH  - Enteroendocrine Cells
MH  - Ileum
MH  - L Cells
MH  - Male
MH  - Mice
PMC - PMC8920715
OTO - NOTNLM
OT  - Ki-67
OT  - carbohydrate
OT  - chicken
OT  - glucagon-like peptides
OT  - proglucagon mRNA
EDAT- 2022/01/05 06:00
MHDA- 2022/03/03 06:00
PMCR- 2022/02/01
CRDT- 2022/01/04 06:02
PHST- 2022/01/05 06:00 [pubmed]
PHST- 2022/03/03 06:00 [medline]
PHST- 2022/01/04 06:02 [entrez]
PHST- 2022/02/01 00:00 [pmc-release]
AID - 21-0572 [pii]
AID - 10.1292/jvms.21-0572 [doi]
PST - ppublish
SO  - J Vet Med Sci. 2022 Mar 1;84(2):265-274. doi: 10.1292/jvms.21-0572. Epub 2021 Dec 
      29.