PMID- 34981018
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220429
IS  - 2322-3480 (Print)
IS  - 2322-3480 (Electronic)
IS  - 2322-3480 (Linking)
VI  - 10
IP  - 3
DP  - 2021 Oct
TI  - Frequency of bla (CTX-M) and bla (TEM) Virulence Genes and Antibiotic Resistance 
      Profiles among Klebsiella pneumoniae Isolates in Urinary Tract Infection (UTI) 
      Samples from Hashtgerd, Iran.
PG  - 412-419
LID - 10.52547/rbmb.10.3.412 [doi]
AB  - BACKGROUND: Klebsiella pneumoniae (K. pneumoniae) is an opportunistic 
      microorganism and one of the most important causes of urinary tract infection. 
      This study aimed to evaluate the frequency of K. pneumoniae producing 
      broad-spectrum beta-lactamase in urinary tract infection and to determine the 
      pattern of drug resistance. METHODS: This study was performed on 50 samples of K. 
      pneumoniae isolated from patients with urinary tract infection referred to the 
      Medical Diagnostic Laboratory in Hashtgerd city. The isolates were first 
      evaluated for antibiotic susceptibility by disk diffusion method according to the 
      method proposed by the Clinical and Laboratory Standards Institute (CLSI). Then 
      phenotypic detection of ESBLS was carried out by the DDST method. The frequency 
      of gene bla (TEM) and bla (CTX-M) was determined by PCR. RESULTS: The highest 
      resistance was observed to ampicillin (94%) and the highest sensitivity was 
      observed to gentamicin (84%). 22 isolates (44%) were positive for ESBLs 
      production. Of the 50 isolates studied, 34% had bla (CTX-M) and 28% had bla (TEM) 
      and 11 (22%) had both genes simultaneously. Also, more than 77% of positive ESBLs 
      isolates had the bla (CTX-M) gene and approximately 63.64% of positive ESBLs 
      isolates had the bla (TEM) gene. CONCLUSION: Given the high prevalence of 
      antibiotic-resistant and ESBL-producing isolates, early identification of these 
      resistant isolates and their follow-up is essential to prevent further outbreaks. 
      It is also important to use appropriate therapeutic strategies and proper and 
      rational administration of antibiotics by physicians.
FAU - Sarshar, Shirin
AU  - Sarshar S
AD  - Master of Sciences (M.Sc.) Microbiology, Department of Microbiology, Faculty of 
      Sciences, Karaj Branch, Islamic Azad University, Karaj, Iran.
FAU - Mirnejad, Reza
AU  - Mirnejad R
AD  - Professor of Medical Bacteriology, Molecular Biology Research Center, Systems 
      biology and poisonings Institute, Baqiyatallah University of Medical Sciences, 
      Tehran, Iran.
FAU - Babapour, Ebrahim
AU  - Babapour E
AD  - Assistant Professor of Microbiology, Department of Microbiology, Karaj Branch, 
      Islamic Azad University, Karaj, Iran.
LA  - eng
PT  - Journal Article
PL  - Iran
TA  - Rep Biochem Mol Biol
JT  - Reports of biochemistry & molecular biology
JID - 101637937
PMC - PMC8718776
OTO - NOTNLM
OT  - Antibiotic Resistance
OT  - ESBLs
OT  - K. pneumoniae
OT  - Urinary Tract Infection
EDAT- 2022/01/05 06:00
MHDA- 2022/01/05 06:01
PMCR- 2021/10/01
CRDT- 2022/01/04 06:07
PHST- 2021/02/07 00:00 [received]
PHST- 2021/04/25 00:00 [accepted]
PHST- 2022/01/04 06:07 [entrez]
PHST- 2022/01/05 06:00 [pubmed]
PHST- 2022/01/05 06:01 [medline]
PHST- 2021/10/01 00:00 [pmc-release]
AID - rbmb-10-412 [pii]
AID - 10.52547/rbmb.10.3.412 [doi]
PST - ppublish
SO  - Rep Biochem Mol Biol. 2021 Oct;10(3):412-419. doi: 10.52547/rbmb.10.3.412.