PMID- 34984861
OWN - NLM
STAT- MEDLINE
DCOM- 20220404
LR  - 20220729
IS  - 1097-0339 (Electronic)
IS  - 8755-1039 (Print)
IS  - 1097-0339 (Linking)
VI  - 50
IP  - 3
DP  - 2022 Mar
TI  - Differential diagnosis of lipoma and atypical lipomatous 
      tumor/well-differentiated liposarcoma by cytological analysis.
PG  - 112-122
LID - 10.1002/dc.24928 [doi]
AB  - BACKGROUND: Adipocytic tumors are the most common soft tissue tumors, with 
      lipomas and atypical lipomatous tumor/well-differentiated liposarcomas (ALT/WDL), 
      which comprise most cases. Preoperative differential diagnosis of lipoma or 
      ALT/WDL can provide important information for decisions regarding treatment. We 
      evaluated the cytological findings of 20 cases of lipoma and ALT/WDL. METHODS: 
      Fluorescence in situ hybridization (FISH) was performed on formalin-fixed 
      paraffin-embedded specimens (FFPE) to examine mouse double minute 2 homolog 
      (MDM2) amplification in all cases. Tissue samples were collected from the center 
      of the surgical materials, stained with Pap, and evaluated for 12 cytological 
      parameters by six cytotechnologists. RESULTS: The findings regarding large 
      atypical cells, multinucleated cells, and nuclear pleomorphism were highly 
      concordant among the cytotechnologists and were associated with MDM2 
      amplification. Large atypical cells, considered a highly specific feature of 
      ALT/WDL, were not observed in lipoma cases. However, the sensitivity of the large 
      atypical cell findings was not high (67%); therefore, comprehensive evaluation of 
      multinucleated cells and pleomorphism is crucial for predicting ALT/WDL 
      diagnosis. FISH of MDM2 on Pap-stained specimens was performed in four cases. In 
      two, the results were similar to those of MDM2 FISH performed on FFPE sections 
      and were reproducible, whereas in the other two, the signal could not be 
      evaluated because of the strong background coloration. CONCLUSIONS: Cytology 
      specimens may be useful for the preoperative diagnosis of adipocytic tumors, 
      particularly if the FISH conditions for Pap-stained specimens and the detection 
      accuracy of MDM2 amplification can be improved.
CI  - (c) 2022 The Authors. Diagnostic Cytopathology published by Wiley Periodicals LLC.
FAU - Sugiyama, Kana
AU  - Sugiyama K
AD  - Department of Clinical Laboratory, Kanagawa Cancer Center, Yokohama, Kanagawa, 
      Japan.
FAU - Washimi, Kota
AU  - Washimi K
AUID- ORCID: 0000-0001-6353-776X
AD  - Department of Pathology, Kanagawa Cancer Center, Yokohama, Kanagawa, Japan.
FAU - Sato, Shinya
AU  - Sato S
AD  - Department of Pathology, Kanagawa Cancer Center, Yokohama, Kanagawa, Japan.
AD  - Molecular Pathology and Genetics Division, Kanagawa Cancer Center Research 
      Institute, Yokohama, Kanagawa, Japan.
FAU - Hiruma, Toru
AU  - Hiruma T
AD  - Department of Musculoskeletal Tumor Surgery, Kanagawa Cancer Center, Yokohama, 
      Kanagawa, Japan.
FAU - Sakai, Mai
AU  - Sakai M
AD  - Department of Clinical Laboratory, Kanagawa Cancer Center, Yokohama, Kanagawa, 
      Japan.
FAU - Okubo, Yoichiro
AU  - Okubo Y
AD  - Department of Pathology, Kanagawa Cancer Center, Yokohama, Kanagawa, Japan.
FAU - Miyagi, Yohei
AU  - Miyagi Y
AD  - Molecular Pathology and Genetics Division, Kanagawa Cancer Center Research 
      Institute, Yokohama, Kanagawa, Japan.
FAU - Yokose, Tomoyuki
AU  - Yokose T
AD  - Department of Pathology, Kanagawa Cancer Center, Yokohama, Kanagawa, Japan.
LA  - eng
GR  - JP 20K16210/Grant-in-aid for Scientific Research/
GR  - 2020-12/Kanagawa Cancer Center Hospital-Research Institute Joint Study/
PT  - Journal Article
DEP - 20220104
PL  - United States
TA  - Diagn Cytopathol
JT  - Diagnostic cytopathology
JID - 8506895
RN  - EC 2.3.2.27 (Proto-Oncogene Proteins c-mdm2)
SB  - IM
MH  - Animals
MH  - Diagnosis, Differential
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/methods
MH  - *Lipoma/diagnosis/genetics/pathology
MH  - *Liposarcoma/diagnosis/genetics/pathology
MH  - Mice
MH  - Proto-Oncogene Proteins c-mdm2/genetics
PMC - PMC9305447
OTO - NOTNLM
OT  - MDM2
OT  - cytology
OT  - fluorescence in situ hybridization
OT  - lipoma
OT  - well-differentiated liposarcoma
COIS- No conflict of interest declared.
EDAT- 2022/01/06 06:00
MHDA- 2022/04/05 06:00
PMCR- 2022/07/22
CRDT- 2022/01/05 06:22
PHST- 2021/12/23 00:00 [revised]
PHST- 2021/11/02 00:00 [received]
PHST- 2021/12/26 00:00 [accepted]
PHST- 2022/01/06 06:00 [pubmed]
PHST- 2022/04/05 06:00 [medline]
PHST- 2022/01/05 06:22 [entrez]
PHST- 2022/07/22 00:00 [pmc-release]
AID - DC24928 [pii]
AID - 10.1002/dc.24928 [doi]
PST - ppublish
SO  - Diagn Cytopathol. 2022 Mar;50(3):112-122. doi: 10.1002/dc.24928. Epub 2022 Jan 4.