PMID- 34986028
OWN - NLM
STAT- MEDLINE
DCOM- 20220217
LR  - 20220426
IS  - 1557-7430 (Electronic)
IS  - 1044-5498 (Linking)
VI  - 41
IP  - 2
DP  - 2022 Feb
TI  - Correlation of Human Leukocyte Antigen-E Genomic Polymorphism with Leukemia and 
      Functional Study of Human Leukocyte Antigen-E Different Type Promoters.
PG  - 235-244
LID - 10.1089/dna.2021.0483 [doi]
AB  - Human leukocyte antigen (HLA)-E is one of the least polymorphic nonclassical 
      major histocompatibility complex (MHC) I genes; its nucleotide variability can 
      affect immune response. In this study, we assess the correlation between HLA-E 
      polymorphism and leukemia and further study the transcriptional activity of 
      promoter variation at nucleotide position-26. A total of 142 healthy blood donors 
      and 111 leukemia patients were collected. The genomic sequence of HLA-E was 
      amplified by high-fidelity reaction system and identified by Sanger and cloning 
      sequencing. The dual luciferase reporter gene assay was used to detect the 
      transcription activity of promoter variation at nucleotide position-26. In the 
      HLA-E genomic sequence results, a total of 16 alleles and 32 genotypes were 
      detected; the HLA-E*01:01:01:06 allele had a significantly lower frequency in 
      leukemia patients than in healthy participants (p = 0.026 < 0.05). And the 
      HLA-E*01:03:02:01, *01:03:02:01 genotype showed the greatest difference in 
      frequency between the two groups of participants (p = 0.028 < 0.05). Eight HLA-E 
      alleles were first reported worldwide in Chinese individuals. The results of the 
      dual luciferase reporter gene experiment showed that the transcription activity 
      of the mutant-type promoter (HLA-E*01:01:01:06 with "T" allele at nucleotide 
      position-26) was significantly lower compared with the wild-type promoter 
      (HLA-E*01:01:01:01 with "G" allele at nucleotide position-26) 
      (p = 0.0242 < 0.05). HLA-E*01:01:01:06 allele has a protective effect against 
      leukemia through decreasing transcription activity by "T" variation at nucleotide 
      position-26.
FAU - Xu, Yun-Ping
AU  - Xu YP
AD  - Shenzhen Institution of Transfusion Medicine, Shenzhen Blood Center, Shenzhen, 
      Guangdong, China.
FAU - Sun, Li-Yan
AU  - Sun LY
AUID- ORCID: 0000-0003-0261-5300
AD  - Shenzhen Institution of Transfusion Medicine, Shenzhen Blood Center, Shenzhen, 
      Guangdong, China.
AD  - Department of Transfusion Medicine, School of Laboratory Medicine and 
      Biotechnology, Southern Medical University, Guangzhou, Guangdong, China.
FAU - Wang, Song-Xing
AU  - Wang SX
AD  - Shenzhen Institution of Transfusion Medicine, Shenzhen Blood Center, Shenzhen, 
      Guangdong, China.
FAU - Hong, Wen-Xu
AU  - Hong WX
AD  - Shenzhen Institution of Transfusion Medicine, Shenzhen Blood Center, Shenzhen, 
      Guangdong, China.
LA  - eng
PT  - Journal Article
DEP - 20220105
PL  - United States
TA  - DNA Cell Biol
JT  - DNA and cell biology
JID - 9004522
RN  - 0 (HLA Antigens)
SB  - IM
MH  - *Genome, Human
MH  - *HLA Antigens/genetics
MH  - Humans
MH  - *Leukemia/genetics
MH  - Polymorphism, Genetic
MH  - Promoter Regions, Genetic
OTO - NOTNLM
OT  - allele and genotype frequency
OT  - genomic full-length
OT  - human leukocyte antigen E molecule
OT  - leukemia
OT  - protective and susceptible gene
OT  - transcription activity
EDAT- 2022/01/06 06:00
MHDA- 2022/02/19 06:00
CRDT- 2022/01/05 17:16
PHST- 2022/01/06 06:00 [pubmed]
PHST- 2022/02/19 06:00 [medline]
PHST- 2022/01/05 17:16 [entrez]
AID - 10.1089/dna.2021.0483 [doi]
PST - ppublish
SO  - DNA Cell Biol. 2022 Feb;41(2):235-244. doi: 10.1089/dna.2021.0483. Epub 2022 Jan 
      5.