PMID- 34987340
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220107
IS  - 1559-3258 (Print)
IS  - 1559-3258 (Electronic)
IS  - 1559-3258 (Linking)
VI  - 19
IP  - 4
DP  - 2021 Oct-Dec
TI  - Stereotactic Body Radiotherapy and Conventional Radiotherapy Induce Cytoskeleton 
      Extension and Enlargement of Cell Morphology in Non-Small Cell Lung Cancer.
PG  - 15593258211064499
LID - 10.1177/15593258211064499 [doi]
LID - 15593258211064499
AB  - Stereotactic body radiotherapy (SBRT) is now widely used in cancer therapy. 
      However, the biological effects of SBRT compared with conventional radiotherapy 
      (CRT) are not clear. The cytoskeleton plays an important role in many biological 
      processes and cellular life activities. The effects of SBRT or CRT on the 
      morphology and cytoskeletal structure of non-small cell lung cancer (NSCLC) cells 
      remain unknown. Based on the biologically equivalent dose (BED) formula, we 
      designed SBRT and CRT fractionation regimens with the same BED. The morphology 
      was captured during radiation, and rhodamine-phalloidin immunofluorescence was 
      used to study the cytoskeleton. A lactate dehydrogenase assay kit was used to 
      determine the cell membrane permeability, and western blot was used to detect the 
      cytoskeleton protein expression levels. The morphology and cytoskeleton expanded 
      after SBRT or CRT, with an increase in cell membrane permeability and stable 
      cytoskeleton protein levels. Besides, different dose of SBRT (10,20,30 Gy) induce 
      similar morphology and cytoskeleton enlargement. Our findings indicate that SBRT 
      and CRT can induce cytoskeleton reorganization and the enlargement of cell 
      morphology (at different rates) in NSCLC. The morphology and cytoskeleton 
      enlargement after SBRT are dose independence.
CI  - (c) The Author(s) 2021.
FAU - Wang, Xiao
AU  - Wang X
AD  - Oncology Center, Department of Medical Oncology, Zhejiang Provincial People's 
      Hospital, People's Hospital of Hangzhou Medical College, Hangzhou, China.
FAU - Lu, Yanwei
AU  - Lu Y
AD  - Oncology Center, Department of Radiation Oncology, Zhejiang Provincial People's 
      Hospital, People's Hospital of Hangzhou Medical College, Hangzhou, China.
FAU - Qin, Zhiquan
AU  - Qin Z
AD  - Oncology Center, Department of Medical Oncology, Zhejiang Provincial People's 
      Hospital, People's Hospital of Hangzhou Medical College, Hangzhou, China.
FAU - Guo, Haiwei
AU  - Guo H
AD  - Department of Head and Neck Surgery, Zhejiang Provincial People's Hospital, 
      People's Hospital of Hangzhou Medical College, Hangzhou, China.
FAU - Chen, Wenjuan
AU  - Chen W
AD  - Department of Psychiatry, Sir Run Run Shaw Hospital, Zhejiang University School 
      of Medicine, Hangzhou, China.
FAU - Ding, Ting
AU  - Ding T
AD  - Department of Endocrinology, Yiyang Central Hospital, Yiyang, China.
FAU - Tang, Jianming
AU  - Tang J
AD  - Key Laboratory of Biotherapy and Regenerative Medicine of Gansu Province, The 
      First Hospital of Lanzhou University, The First Clinical Medical College of 
      Lanzhou University, Lanzhou, China.
FAU - Zhang, Haibo
AU  - Zhang H
AD  - Oncology Center, Department of Radiation Oncology, Zhejiang Provincial People's 
      Hospital, People's Hospital of Hangzhou Medical College, Hangzhou, China.
LA  - eng
PT  - Journal Article
DEP - 20211217
PL  - United States
TA  - Dose Response
JT  - Dose-response : a publication of International Hormesis Society
JID - 101308899
PMC - PMC8725237
OTO - NOTNLM
OT  - conventional radiotherapy
OT  - cytoskeleton
OT  - morphology
OT  - non-small cell lung cancer
OT  - stereotactic body radiotherapy
COIS- Declaration of Conflicting Interests: The author(s) declared no potential 
      conflicts of interest with respect to the research, authorship, and/or 
      publication of this article.
EDAT- 2022/01/07 06:00
MHDA- 2022/01/07 06:01
PMCR- 2021/12/17
CRDT- 2022/01/06 05:51
PHST- 2022/01/06 05:51 [entrez]
PHST- 2022/01/07 06:00 [pubmed]
PHST- 2022/01/07 06:01 [medline]
PHST- 2021/12/17 00:00 [pmc-release]
AID - 10.1177_15593258211064499 [pii]
AID - 10.1177/15593258211064499 [doi]
PST - epublish
SO  - Dose Response. 2021 Dec 17;19(4):15593258211064499. doi: 
      10.1177/15593258211064499. eCollection 2021 Oct-Dec.