PMID- 34990977
OWN - NLM
STAT- MEDLINE
DCOM- 20220201
LR  - 20220201
IS  - 1618-0984 (Electronic)
IS  - 0723-2020 (Linking)
VI  - 45
IP  - 1
DP  - 2022 Jan
TI  - Mycoplasma tauri sp. nov. isolated from the bovine genital tract.
PG  - 126292
LID - S0723-2020(21)00115-6 [pii]
LID - 10.1016/j.syapm.2021.126292 [doi]
AB  - Since 2006, a Mycoplasma species unidentifiable to the species level has been 
      regularly isolated from the semen and prepuce of apparently healthy bulls, and 
      occasionally from cattle displaying inflammatory disease of the genital tract. 
      Seven of these Mycoplasma isolates were subjected to a comprehensive taxonomic 
      study. The strains investigated grew well in modified Hayflick's medium and 
      colonies on agar exhibited typical fried egg morphology and produced 'film and 
      spots'. Transmission electron microscopy revealed a cell morphology 
      characteristic of mycoplasmas with spherically shaped cells bounded by a 
      bi-layered cell membrane. The strains studied neither produced acid from sugar 
      carbon sources nor did hydrolyse arginine or urea, and genome annotation 
      indicated that organic acids (pyruvate, lactate) are used as energy sources. 
      Phylogenetic analyses of 16S rRNA gene sequences, the 16S-23S intergenic spacer 
      region, and partial rpoB gene and protein sequences placed the strains within the 
      Mycoplasma (M.) bovis cluster of the Hominis group with M. primatum, M. 
      agalactiae, and M. bovis being their closest relatives. Genomic information 
      including whole-genome similarity metrics (ANIb, ANIm, TETRA, dDDH, AAI) and 
      phylogenomics, proteomic features revealed by matrix-assisted laser desorption 
      ionization time of flight (MALDI-ToF) mass spectrometry as well as serological 
      reactions and polar lipid profiling strongly indicated that the strains examined 
      were representatives of a hitherto unclassified species of genus Mycoplasma, for 
      which the name Mycoplasma tauri sp. nov. with type strain Zaradi2(T) (=ATCC 
      BAA-1891(T) = DSM 22451(T)) is proposed.
CI  - Copyright (c) 2021 The Author(s). Published by Elsevier GmbH.. All rights reserved.
FAU - Spergser, Joachim
AU  - Spergser J
AD  - Institute of Microbiology, University of Veterinary Medicine, Vienna, Austria. 
      Electronic address: joachim.spergser@vetmeduni.ac.at.
FAU - DeSoye, Philippe
AU  - DeSoye P
AD  - Institute of Microbiology, University of Veterinary Medicine, Vienna, Austria.
FAU - Ruppitsch, Werner
AU  - Ruppitsch W
AD  - Austrian Agency for Health and Food Safety (AGES), Institute of Medical 
      Microbiology and Hygiene, Vienna, Austria.
FAU - Cabal Rosel, Adriana
AU  - Cabal Rosel A
AD  - Austrian Agency for Health and Food Safety (AGES), Institute of Medical 
      Microbiology and Hygiene, Vienna, Austria.
FAU - Dinhopl, Nora
AU  - Dinhopl N
AD  - Institute of Pathology, University of Veterinary Medicine, Vienna, Austria.
FAU - Szostak, Michael P
AU  - Szostak MP
AD  - Institute of Microbiology, University of Veterinary Medicine, Vienna, Austria.
FAU - Loncaric, Igor
AU  - Loncaric I
AD  - Institute of Microbiology, University of Veterinary Medicine, Vienna, Austria.
FAU - Chopra-Dewasthaly, Rohini
AU  - Chopra-Dewasthaly R
AD  - Institute of Microbiology, University of Veterinary Medicine, Vienna, Austria.
FAU - Busse, Hans-Jurgen
AU  - Busse HJ
AD  - Institute of Microbiology, University of Veterinary Medicine, Vienna, Austria.
LA  - eng
PT  - Journal Article
DEP - 20211225
PL  - Germany
TA  - Syst Appl Microbiol
JT  - Systematic and applied microbiology
JID - 8306133
RN  - 0 (DNA, Bacterial)
RN  - 0 (RNA, Ribosomal, 16S)
SB  - IM
MH  - Animals
MH  - Bacterial Typing Techniques
MH  - Cattle
MH  - DNA, Bacterial/genetics
MH  - Genitalia
MH  - Male
MH  - *Mycoplasma/genetics
MH  - Phylogeny
MH  - Proteomics
MH  - RNA, Ribosomal, 16S/genetics
MH  - Sequence Analysis, DNA
OTO - NOTNLM
OT  - Bovine genital tract
OT  - Mycoplasma tauri sp. nov
OT  - Novel mycoplasma
EDAT- 2022/01/07 06:00
MHDA- 2022/02/02 06:00
CRDT- 2022/01/06 20:23
PHST- 2021/11/14 00:00 [received]
PHST- 2021/12/11 00:00 [revised]
PHST- 2021/12/17 00:00 [accepted]
PHST- 2022/01/07 06:00 [pubmed]
PHST- 2022/02/02 06:00 [medline]
PHST- 2022/01/06 20:23 [entrez]
AID - S0723-2020(21)00115-6 [pii]
AID - 10.1016/j.syapm.2021.126292 [doi]
PST - ppublish
SO  - Syst Appl Microbiol. 2022 Jan;45(1):126292. doi: 10.1016/j.syapm.2021.126292. 
      Epub 2021 Dec 25.