PMID- 34992597
OWN - NLM
STAT- MEDLINE
DCOM- 20220214
LR  - 20221221
IS  - 1664-3224 (Electronic)
IS  - 1664-3224 (Linking)
VI  - 12
DP  - 2021
TI  - Epitope-Based Vaccine of a Brucella abortus Putative Small RNA Target Induces 
      Protection and Less Tissue Damage in Mice.
PG  - 778475
LID - 10.3389/fimmu.2021.778475 [doi]
LID - 778475
AB  - Brucella spp. are Gram-negative, facultative intracellular bacteria that cause 
      brucellosis in humans and animals. Currently available live attenuated vaccines 
      against brucellosis still have drawbacks. Therefore, subunit vaccines, produced 
      using epitope-based antigens, have the advantage of being safe, cost-effective 
      and efficacious. Here, we identified B. abortus small RNAs expressed during early 
      infection with bone marrow-derived macrophages (BMDMs) and an apolipoprotein 
      N-acyltransferase (Int) was identified as the putative target of the greatest 
      expressed small RNA. Decreased expression of Int was observed during BMDM 
      infection and the protein sequence was evaluated to rationally select a putative 
      immunogenic epitope by immunoinformatic, which was explored as a vaccinal 
      candidate. C57BL/6 mice were immunized and challenged with B. abortus, showing 
      lower recovery in the number of viable bacteria in the liver, spleen, and 
      axillary lymph node and greater production of IgG and fractions when compared to 
      non-vaccinated mice. The vaccinated and infected mice showed the increased 
      expression of TNF-alpha, IFN-gamma, and IL-6 following expression of the 
      anti-inflammatory genes IL-10 and TGF-beta in the liver, justifying the reduction in 
      the number and size of the observed granulomas. BMDMs stimulated with splenocyte 
      supernatants from vaccinated and infected mice increase the CD86+ marker, as well 
      as expressing greater amounts of iNOS and the consequent increase in NO 
      production, suggesting an increase in the phagocytic and microbicidal capacity of 
      these cells to eliminate the bacteria.
CI  - Copyright (c) 2021 Oliveira, Brancaglion, Santos, Araujo, Novaes, Santos, Oliveira, 
      Corsetti and de Almeida.
FAU - Oliveira, Karen Cristina
AU  - Oliveira KC
AD  - Laboratory of Molecular Biology of Microorganisms, Federal University of Alfenas, 
      Alfenas, Brazil.
FAU - Brancaglion, Gustavo Andrade
AU  - Brancaglion GA
AD  - Laboratory of Molecular Biology of Microorganisms, Federal University of Alfenas, 
      Alfenas, Brazil.
FAU - Santos, Natalia C M
AU  - Santos NCM
AD  - Laboratory of Molecular Biology of Microorganisms, Federal University of Alfenas, 
      Alfenas, Brazil.
FAU - Araujo, Leonardo P
AU  - Araujo LP
AD  - Laboratory of Molecular Biology of Microorganisms, Federal University of Alfenas, 
      Alfenas, Brazil.
FAU - Novaes, Evandro
AU  - Novaes E
AD  - Department of Biology, Federal University of Lavras, Lavras, Brazil.
FAU - Santos, Renato de Lima
AU  - Santos RL
AD  - Department of Clinic and Veterinary Surgery, Veterinary School, Federal 
      University of Minas Gerais, Belo Horizonte, Brazil.
FAU - Oliveira, Sergio Costa
AU  - Oliveira SC
AD  - Department of Biochemistry and Immunology, Federal University of Minas Gerais, 
      Belo Horizonte, Brazil.
FAU - Corsetti, Patricia Paiva
AU  - Corsetti PP
AD  - Laboratory of Molecular Biology of Microorganisms, Federal University of Alfenas, 
      Alfenas, Brazil.
FAU - de Almeida, Leonardo Augusto
AU  - de Almeida LA
AD  - Laboratory of Molecular Biology of Microorganisms, Federal University of Alfenas, 
      Alfenas, Brazil.
LA  - eng
GR  - R01 AI116453/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20211221
PL  - Switzerland
TA  - Front Immunol
JT  - Frontiers in immunology
JID - 101560960
RN  - 0 (Antigens, Bacterial)
RN  - 0 (Brucella Vaccine)
RN  - 0 (RNA, Bacterial)
RN  - 0 (Vaccines, Subunit)
RN  - EC 2.3.- (Acyltransferases)
RN  - EC 2.3.1.- (apolipoprotein N-acyltransferase)
SB  - IM
MH  - Acyltransferases/genetics
MH  - Animals
MH  - Antigens, Bacterial/genetics/immunology
MH  - Bacterial Zoonoses/immunology/microbiology/*prevention & control
MH  - Brucella Vaccine/administration & dosage/genetics/*immunology
MH  - Brucella abortus/genetics/*immunology
MH  - Brucellosis/immunology/microbiology/*prevention & control
MH  - Computer Simulation
MH  - Disease Models, Animal
MH  - Epitope Mapping/methods
MH  - Humans
MH  - Immunogenicity, Vaccine
MH  - Macrophages/immunology/microbiology
MH  - Mice
MH  - Primary Cell Culture
MH  - RNA, Bacterial/genetics/isolation & purification
MH  - Vaccines, Subunit/administration & dosage/immunology
PMC - PMC8724193
OTO - NOTNLM
OT  - Brucella abortus
OT  - apolipoprotein N-acyltransferase
OT  - brucellosis
OT  - immune response
OT  - reverse vaccinology
OT  - vaccine
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2022/01/08 06:00
MHDA- 2022/02/15 06:00
PMCR- 2021/01/01
CRDT- 2022/01/07 06:23
PHST- 2021/09/16 00:00 [received]
PHST- 2021/12/03 00:00 [accepted]
PHST- 2022/01/07 06:23 [entrez]
PHST- 2022/01/08 06:00 [pubmed]
PHST- 2022/02/15 06:00 [medline]
PHST- 2021/01/01 00:00 [pmc-release]
AID - 10.3389/fimmu.2021.778475 [doi]
PST - epublish
SO  - Front Immunol. 2021 Dec 21;12:778475. doi: 10.3389/fimmu.2021.778475. eCollection 
      2021.