PMID- 34993982
OWN - NLM
STAT- MEDLINE
DCOM- 20220419
LR  - 20220419
IS  - 1099-0801 (Electronic)
IS  - 0269-3879 (Linking)
VI  - 36
IP  - 5
DP  - 2022 May
TI  - Determination of multiple active constituents in Da-Huang-Xiao-Shi decoction 
      using HPLC-LTQ-Orbitrap mass spectrometry: Application in comparing the 
      differences in the formula and its constituent herbs.
PG  - e5324
LID - 10.1002/bmc.5324 [doi]
AB  - Da-Huang-Xiao-Shi decoction (DHXSD) is a traditional Chinese medicine formula and 
      is used to treat cholestasis. In this study, we developed a reliable and 
      comprehensive HPLC coupled with a linear ion trap-Orbitrap mass spectrometry 
      method for the separation and determination of 21 components including six 
      alkaloids, five anthraquinones, three tannins, three terpenes, two iridoid 
      glycosides, one organic acid and one flavonoid in DHXSD. A C18 column was eluted 
      using a gradient mobile phase at a flow rate of 1 ml/min. Detection was operated 
      with an electrospray ionization source in positive and negative ion modes using 
      selective ion monitoring. The calibration curves for all analytes showed good 
      linearity (r > 0.9901), and the inter- and intra-day precision did not exceed 
      4.98%. The recovery, repeatability and stability were also within the acceptable 
      limits. The method was successfully applied to determine multiple active 
      constituents in DHXSD and its constituent herbs. Compared with Da Huang, the 
      total contents of the five anthraquinones were significantly higher in DHXSD. 
      However, the changes in the components from Zhi Zi/Huang Bo were complicated in 
      DHXSD. The study could serve as a fundamental reference for establishing 
      comprehensive DHXSD quality control measures and be helpful to understand some 
      compatibility laws of DHXSD.
CI  - (c) 2022 John Wiley & Sons, Ltd.
FAU - Li, Hongyu
AU  - Li H
AD  - Department of Pharmacology, Shanghai University of Traditional Chinese Medicine, 
      Shanghai, China.
FAU - Jin, Jingyi
AU  - Jin J
AD  - Department of Pharmacology, Shanghai University of Traditional Chinese Medicine, 
      Shanghai, China.
FAU - Xue, Haoyu
AU  - Xue H
AD  - Department of Pharmacology, Shanghai University of Traditional Chinese Medicine, 
      Shanghai, China.
FAU - Li, Yuanyuan
AU  - Li Y
AD  - Department of Pharmacology, Shanghai University of Traditional Chinese Medicine, 
      Shanghai, China.
FAU - Wang, Tianming
AU  - Wang T
AD  - Department of Pharmacology, Shanghai University of Traditional Chinese Medicine, 
      Shanghai, China.
FAU - Shi, Rong
AU  - Shi R
AD  - Department of Pharmacology, Shanghai University of Traditional Chinese Medicine, 
      Shanghai, China.
AD  - Science and Technology Experimental Center, Shanghai University of Traditional 
      Chinese Medicine, Shanghai, China.
FAU - Ma, Yueming
AU  - Ma Y
AUID- ORCID: 0000-0001-7701-0846
AD  - Department of Pharmacology, Shanghai University of Traditional Chinese Medicine, 
      Shanghai, China.
LA  - eng
GR  - 2018JP011/Shanghai Municipal Commission of Health and Family Planning/
GR  - ZYCC2019019/Shanghai municipal Health Commission/
PT  - Journal Article
DEP - 20220216
PL  - England
TA  - Biomed Chromatogr
JT  - Biomedical chromatography : BMC
JID - 8610241
RN  - 0 (Anthraquinones)
RN  - 0 (Da-Huang-Xiao-Shi decoction)
RN  - 0 (Drugs, Chinese Herbal)
SB  - IM
MH  - Anthraquinones/chemistry
MH  - Chromatography, High Pressure Liquid/methods
MH  - *Drugs, Chinese Herbal/chemistry
MH  - Mass Spectrometry/methods
OTO - NOTNLM
OT  - Da-Huang-Xiao-Shi decoction
OT  - HPLC-LTQ-Orbitrap MS
OT  - quality evaluation
OT  - traditional Chinese medicine
EDAT- 2022/01/08 06:00
MHDA- 2022/04/20 06:00
CRDT- 2022/01/07 06:49
PHST- 2021/12/21 00:00 [revised]
PHST- 2021/07/04 00:00 [received]
PHST- 2021/12/22 00:00 [accepted]
PHST- 2022/01/08 06:00 [pubmed]
PHST- 2022/04/20 06:00 [medline]
PHST- 2022/01/07 06:49 [entrez]
AID - 10.1002/bmc.5324 [doi]
PST - ppublish
SO  - Biomed Chromatogr. 2022 May;36(5):e5324. doi: 10.1002/bmc.5324. Epub 2022 Feb 16.