PMID- 34994136
OWN - NLM
STAT- MEDLINE
DCOM- 20220110
LR  - 20220110
IS  - 1001-5302 (Print)
IS  - 1001-5302 (Linking)
VI  - 46
IP  - 24
DP  - 2021 Dec
TI  - [Qualitative and quantitative analysis of alkaloids in Eurycoma longifolia by 
      HPLC-Q-TOF-MS combined with UPLC-QQQ-MS/MS].
PG  - 6435-6446
LID - 10.19540/j.cnki.cjcmm.20210802.202 [doi]
AB  - A sensitive and efficient method was established and validated for qualitative 
      and quantitative analysis of total alkaloids from the extract of Eurycoma 
      longifolia by high-performance liquid chromatography coupled with quadrupole 
      time-of-flight mass spectrometry(HPLC-Q-TOF-MS) combined with ultra-performance 
      liquid chromatography coupled with triple quadrupole tandem mass 
      spectrometry(UPLC-QQQ-MS/MS). The HPLC-Q-TOF-MS conditions are as follows: Welch 
      Ultimate XB-C_(18) column(4.6 mmx250 mm, 5 mum) with acetonitrile(containing 0.1% 
      formic acid)-0.1% formic acid in water as mobile phase for gradient elution. The 
      UPLC-QQQ-MS/MS conditions are as below: Agilent Eclipse Plus C_(18) column(2.1 
      mmx50 mm, 1.8 mum) with acetonitrile(containing 0.1% formic acid) and 0.1% formic 
      acid in water as mobile phase for gradient elution. MS data were collected by 
      electrospray ionization in positive ion mode. According to the comparison with 
      reference standards and the accurate masses of molecules, a total of 17 alkaloids 
      in E. longifolia extract were identified by HPLC-Q-TOF-MS. The UPLC-QQQ-MS/MS 
      quantitative analysis result of 3 alkaloids showed that the linear ranges of them 
      were good(r>/=0.999 7) and the overall recoveries ranged from 108.8%-110.2%, with 
      RSDs of 2.9%-5.3%. The method is accurate, reliable, and efficient, which can 
      comprehensively reflect the constituents and content of alkaloids in E. 
      longifolia. The result can serve as a reference for further elucidating its 
      therapeutic material basis and quality control.
FAU - Chen, Xiu-Ming
AU  - Chen XM
AD  - Fujian Provincial Key Laboratory of Screening for Novel Microbial Products, 
      Fujian Institute of Microbiology Fuzhou 350007, China.
FAU - Lin, Bin
AU  - Lin B
AD  - Fujian Provincial Key Laboratory of Screening for Novel Microbial Products, 
      Fujian Institute of Microbiology Fuzhou 350007, China.
FAU - Fang, Dong-Sheng
AU  - Fang DS
AD  - Fujian Provincial Key Laboratory of Screening for Novel Microbial Products, 
      Fujian Institute of Microbiology Fuzhou 350007, China.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhongguo Zhong Yao Za Zhi
JT  - Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese 
      materia medica
JID - 8913656
RN  - 0 (Alkaloids)
SB  - IM
MH  - *Alkaloids
MH  - Chromatography, High Pressure Liquid
MH  - Chromatography, Liquid
MH  - *Eurycoma
MH  - Tandem Mass Spectrometry
OTO - NOTNLM
OT  - Eurycoma longifolia
OT  - HPLC-Q-TOF-MS
OT  - UPLC-QQQ-MS/MS
OT  - alkaloid
OT  - qualitative analysis
OT  - quantitative analysis
EDAT- 2022/01/08 06:00
MHDA- 2022/01/11 06:00
CRDT- 2022/01/07 06:59
PHST- 2022/01/07 06:59 [entrez]
PHST- 2022/01/08 06:00 [pubmed]
PHST- 2022/01/11 06:00 [medline]
AID - 10.19540/j.cnki.cjcmm.20210802.202 [doi]
PST - ppublish
SO  - Zhongguo Zhong Yao Za Zhi. 2021 Dec;46(24):6435-6446. doi: 
      10.19540/j.cnki.cjcmm.20210802.202.