PMID- 34995866 OWN - NLM STAT- MEDLINE DCOM- 20220127 LR - 20220127 IS - 1879-1514 (Electronic) IS - 0166-445X (Linking) VI - 243 DP - 2022 Feb TI - Molecular insight into the hepatopancreas of oriental river prawn (Macrobrachium nipponense) in response to residual chlorine stimulus. PG - 106052 LID - S0166-445X(21)00312-X [pii] LID - 10.1016/j.aquatox.2021.106052 [doi] AB - Chlorine or chloric disinfectants are cost-effective disinfectants, which are widely used to disinfect domestic and industrial water. The residual chlorine levels in some of these waters have been proven toxic to several aquatic organisms; however, the molecular mechanisms of toxicity of residual chlorine to aquatic crustaceans, including Macrobrachium nipponense, an economically important freshwater prawn native to Asian countries, have not been investigated to date. Here, M. nipponense was exposed to 0.53 mg/L of residual chlorine, and comparative transcriptomics analyses were performed to determine their response mechanisms at the molecular level. Residual chlorine caused lethal effects on prawns. Furthermore, a total of 940 differentially expressed genes (DEGs), including 501 up-regulated and 439 down-regulated genes, were identified after 48 h of residual chlorine exposure compared to the control group. After enrichment analysis of GO (Gene Ontology) functions and KEGG (Kyoto Encyclopedia of Genes and Genomes) pathways, identified DEGs were demonstrated to be associated with a variety of functions including exerting "oxidoreductase activity", and participating in "oxidation-reduction process". In addition, cytochrome P450 family 1 subfamily A1 (CYP1A1), glutathione S-transferase (GST), and glucuronosyltransferase (UGT) were enriched in the pathway of metabolism of xenobiotics by cytochrome P450. Furthermore, protein-protein interaction (PPI) network analysis revealed interactions among actin beta/gamma 1 (ACTB_G1) gene encoding protein and a series of multiple functional DEGs (e.g., hexokinase (HK), fructose 1,6-biphosphate-aldolase A (ALDOA), cytochrome c (CYC), and elongation factor 1-alpha (EEF1A)) encoding proteins. This study laid a theoretical foundation for safety evaluation of chlorinated aquatic water and further investigation of the toxicity of chlorination to M. nipponense. CI - Copyright (c) 2021 Elsevier B.V. All rights reserved. FAU - Zhu, Peng AU - Zhu P AD - Shandong Sport University, Jinan 250102, China; Department of Aquaculture Research Lab, College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai'an 271018, China; Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai'an 271018, China. Electronic address: 824424443@qq.com. FAU - Sun, Yaojiadai AU - Sun Y AD - Shandong Sport University, Jinan 250102, China; Library, Shandong Agricultural University, Tai'an 271018, China. Electronic address: 429345863@qq.com. FAU - Wang, Hui AU - Wang H AD - Department of Aquaculture Research Lab, College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai'an 271018, China. Electronic address: wanghui2328@sdau.edu.cn. FAU - Ji, Xiangshan AU - Ji X AD - Department of Aquaculture Research Lab, College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai'an 271018, China; Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai'an 271018, China. Electronic address: xsji@sdau.edu.cn. FAU - Zeng, Yongqing AU - Zeng Y AD - Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai'an 271018, China. Electronic address: yqzeng@sdau.edu.cn. LA - eng PT - Journal Article DEP - 20211212 PL - Netherlands TA - Aquat Toxicol JT - Aquatic toxicology (Amsterdam, Netherlands) JID - 8500246 RN - 0 (Water Pollutants, Chemical) RN - 4R7X1O2820 (Chlorine) SB - IM MH - Animals MH - Chlorine MH - Hepatopancreas MH - *Palaemonidae/genetics MH - Transcriptome MH - *Water Pollutants, Chemical/toxicity OTO - NOTNLM OT - Freshwater prawns OT - Macrobrachium nipponense OT - Mechanisms underlying toxicity OT - Residual chlorine stimulus OT - Transcriptome sequencing EDAT- 2022/01/08 06:00 MHDA- 2022/01/28 06:00 CRDT- 2022/01/07 20:18 PHST- 2020/11/23 00:00 [received] PHST- 2021/11/25 00:00 [revised] PHST- 2021/12/10 00:00 [accepted] PHST- 2022/01/08 06:00 [pubmed] PHST- 2022/01/28 06:00 [medline] PHST- 2022/01/07 20:18 [entrez] AID - S0166-445X(21)00312-X [pii] AID - 10.1016/j.aquatox.2021.106052 [doi] PST - ppublish SO - Aquat Toxicol. 2022 Feb;243:106052. doi: 10.1016/j.aquatox.2021.106052. Epub 2021 Dec 12.