PMID- 34996232
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220113
IS  - 2576-6422 (Electronic)
IS  - 2576-6422 (Linking)
VI  - 1
IP  - 5
DP  - 2018 Nov 19
TI  - Antiviral Activity of Graphene Oxide-Silver Nanocomposites by Preventing Viral 
      Entry and Activation of the Antiviral Innate Immune Response.
PG  - 1286-1293
LID - 10.1021/acsabm.8b00154 [doi]
AB  - Developing nanomaterials-based antimicrobial agents has shown a widespread 
      promise. In this study, silver nanoparticle-modified graphene oxide (GO-AgNPs) 
      nanocomposites were self-assembled via interfacial electrostatic force. By using 
      the porcine reproductive and respiratory syndrome virus (PRRSV) as a pattern, the 
      antiviral effect of the as-prepared GO-AgNPs nanocomposites on the replication of 
      virus was investigated. The results indicated that exposure with GO-AgNPs 
      nanocomposites could obviously suppress PRRSV infection. It was found that 
      GO-AgNPs nanocomposites exhibited a better inhibitory effect compared with AgNPs 
      and GO. By selecting the porcine epidemic diarrhea virus (PEDV) as a contrast 
      virus, GO-AgNPs nanocomposites were proven to have a broad antiviral activity. 
      Mechanism studies showed that GO-AgNPs nanocomposites might prevent PRRSV from 
      entering the host cells, with 59.2% inhibition efficiency. Meanwhile, GO-AgNPs 
      nanocomposite treatment enhances the production of interferon-alpha (IFN-alpha) and 
      IFN-stimulating genes (ISGs), which can directly inhibit the proliferation of 
      virus. Taken together, this study reports a new type of antiviral agent and 
      provides a promising pharmaceutical agent for treating infection by the highly 
      pathogenic PRRSV. Moreover, it may provide novel ideas for the research and 
      development of antiviral formulations based on nanocomposites and extend their 
      applications in biological systems.
FAU - Du, Ting
AU  - Du T
AD  - State Key Laboratory of Agricultural Microbiology, College of Food Science and 
      Technology, College of Science, Huazhong Agricultural University, Wuhan 430070, 
      People's Republic of China.
FAU - Lu, Jian
AU  - Lu J
AD  - State Key Laboratory of Agricultural Microbiology, College of Food Science and 
      Technology, College of Science, Huazhong Agricultural University, Wuhan 430070, 
      People's Republic of China.
FAU - Liu, Lingzhi
AU  - Liu L
AD  - State Key Laboratory of Agricultural Microbiology, College of Food Science and 
      Technology, College of Science, Huazhong Agricultural University, Wuhan 430070, 
      People's Republic of China.
FAU - Dong, Nan
AU  - Dong N
AD  - State Key Laboratory of Agricultural Microbiology, College of Veterinary 
      Medicine, Huazhong Agricultural University, Wuhan 430070, People's Republic of 
      China.
FAU - Fang, Liurong
AU  - Fang L
AD  - State Key Laboratory of Agricultural Microbiology, College of Veterinary 
      Medicine, Huazhong Agricultural University, Wuhan 430070, People's Republic of 
      China.
FAU - Xiao, Shaobo
AU  - Xiao S
AUID- ORCID: 0000-0003-0023-9188
AD  - State Key Laboratory of Agricultural Microbiology, College of Veterinary 
      Medicine, Huazhong Agricultural University, Wuhan 430070, People's Republic of 
      China.
FAU - Han, Heyou
AU  - Han H
AUID- ORCID: 0000-0001-9406-0722
AD  - State Key Laboratory of Agricultural Microbiology, College of Food Science and 
      Technology, College of Science, Huazhong Agricultural University, Wuhan 430070, 
      People's Republic of China.
LA  - eng
PT  - Journal Article
DEP - 20181108
PL  - United States
TA  - ACS Appl Bio Mater
JT  - ACS applied bio materials
JID - 101729147
SB  - IM
EIN - ACS Appl Bio Mater. 2020 May 18;3(5):3440. PMID: 35025412
OTO - NOTNLM
OT  - GO-AgNPs nanocomposites
OT  - antiviral
OT  - entry
OT  - interferon-stimulating genes
OT  - interferon-alpha
EDAT- 2018/11/19 00:00
MHDA- 2018/11/19 00:01
CRDT- 2022/01/08 01:00
PHST- 2022/01/08 01:00 [entrez]
PHST- 2018/11/19 00:00 [pubmed]
PHST- 2018/11/19 00:01 [medline]
AID - 10.1021/acsabm.8b00154 [doi]
PST - ppublish
SO  - ACS Appl Bio Mater. 2018 Nov 19;1(5):1286-1293. doi: 10.1021/acsabm.8b00154. Epub 
      2018 Nov 8.