PMID- 34999279
OWN - NLM
STAT- MEDLINE
DCOM- 20220321
LR  - 20220321
IS  - 1618-0631 (Electronic)
IS  - 0344-0338 (Linking)
VI  - 230
DP  - 2022 Feb
TI  - The clinical significance of transcription factor WD repeat and HMG-box DNA 
      binding protein 1 in laryngeal squamous cell carcinoma and its potential 
      molecular mechanism.
PG  - 153751
LID - S0344-0338(21)00412-X [pii]
LID - 10.1016/j.prp.2021.153751 [doi]
AB  - BACKGROUND: Currently, high expression of WD repeat and HMG-box DNA binding 
      protein 1 (WDHD1) has been found in a variety of tumors; but there is no research 
      has been conducted concerning the expression of WDHD1 in laryngeal squamous cell 
      carcinoma (LSCC). Our purpose is to investigate the expression and the latent 
      mechanism of WDHD1 in LSCC. METHODS: Firstly, 9 data sets from the Gene 
      Expression Omnibus (GEO), The Cancer Genome Atlas (TCGA), and ArrayExpress were 
      statistically analyzed to explore the expression of WDHD1 in LSCC; 
      immunohistochemistry was performed in 79 LSCC tissues and 44 non-cancer tissues 
      to further verify the result. In addition, the target gene of WDHD1 was predicted 
      and immunohistochemistry was used to detect the expression of the target gene. 
      The potential mechanism of WDHD1 in LSCC was investigated by Gene Ontology (GO), 
      Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses and 
      protein-protein interaction network (PPI). RESULTS: The WDHD1 mRNA was expressed 
      at higher levels in the LSCC tissue than in the normal tissue (SMD=1.90, 95% 
      CI=1.50-2.30); and the results of immunohistochemistry were consistent with the 
      conclusion. Using chip-seq analysis, we found that S-phase kinase-associated 
      protein 2 (Skp2) had a significant binding peak with WDHD1, and the expression of 
      these two genes was significantly positively correlated. Immunohistochemistry 
      showed that Skp2 was also highly expressed in LSCC. In addition, GO and KEGG 
      analysis revealed the WDHD1 positively correlated genes was closely related to 
      cell cycle, and PPI analysis identified 10 hub genes: COL7A1, COL4A2, COL4A1, 
      COL4A6, COL11A1, COL5A2, COL1A1, COL13A1, COL8A1 and COL10A1, which may be 
      critical to the progression of LSCC. CONCLUSIONS: WDHD1 was overexpressed in LSCC 
      tissues. Meanwhile, WDHD1 and its target gene Skp2 for transcriptional regulation 
      may play a role in the progression of LSCC by regulating the cell cycle.
CI  - Copyright (c) 2022 The Authors. Published by Elsevier GmbH.. All rights reserved.
FAU - Wu, Ji-Yun
AU  - Wu JY
AD  - Department of Radiotherapy, First Affiliated Hospital of Guangxi Medical 
      University, 6 Shuangyong Rd, Nanning, Guangxi Zhuang Autonomous Region 530021, PR 
      China.
FAU - Lan, Xiao-Lu
AU  - Lan XL
AD  - Department of Traditional Chinese Medicine, YiZhou District Hospital of 
      Traditional Chinese Medicine, Jiulong Road, YiZhou, Hechi, Guangxi Zhuang 
      Autonomous Region 546399, PR China.
FAU - Yan, Dong-Mei
AU  - Yan DM
AD  - Department of Radiotherapy, First Affiliated Hospital of Guangxi Medical 
      University, 6 Shuangyong Rd, Nanning, Guangxi Zhuang Autonomous Region 530021, PR 
      China.
FAU - Fang, Ye-Ying
AU  - Fang YY
AD  - Department of Radiotherapy, First Affiliated Hospital of Guangxi Medical 
      University, 6 Shuangyong Rd, Nanning, Guangxi Zhuang Autonomous Region 530021, PR 
      China.
FAU - Peng, Yun-Xi
AU  - Peng YX
AD  - Department of Radiotherapy, First Affiliated Hospital of Guangxi Medical 
      University, 6 Shuangyong Rd, Nanning, Guangxi Zhuang Autonomous Region 530021, PR 
      China.
FAU - Liang, Fei-Fei
AU  - Liang FF
AD  - Department of Radiotherapy, First Affiliated Hospital of Guangxi Medical 
      University, 6 Shuangyong Rd, Nanning, Guangxi Zhuang Autonomous Region 530021, PR 
      China.
FAU - Jiang, Li
AU  - Jiang L
AD  - Department of Radiotherapy, First Affiliated Hospital of Guangxi Medical 
      University, 6 Shuangyong Rd, Nanning, Guangxi Zhuang Autonomous Region 530021, PR 
      China.
FAU - Huang, Su-Ning
AU  - Huang SN
AD  - Department of Radiotherapy, Guangxi Medical University Cancer Hospital, No.71 
      Hedi Rd, Nanning, Guangxi Zhuang Autonomous Region 530021, PR China.
FAU - Mo, Miao
AU  - Mo M
AD  - Department of Radiotherapy, First Affiliated Hospital of Guangxi Medical 
      University, 6 Shuangyong Rd, Nanning, Guangxi Zhuang Autonomous Region 530021, PR 
      China.
FAU - Lin, Cai-Xing
AU  - Lin CX
AD  - Department of Radiotherapy, First Affiliated Hospital of Guangxi Medical 
      University, 6 Shuangyong Rd, Nanning, Guangxi Zhuang Autonomous Region 530021, PR 
      China.
FAU - Niu, Yi-Tong
AU  - Niu YT
AD  - Department of Radiotherapy, First Affiliated Hospital of Guangxi Medical 
      University, 6 Shuangyong Rd, Nanning, Guangxi Zhuang Autonomous Region 530021, PR 
      China.
FAU - Wu, Xiao-Wei
AU  - Wu XW
AD  - Department of Radiotherapy, First Affiliated Hospital of Guangxi Medical 
      University, 6 Shuangyong Rd, Nanning, Guangxi Zhuang Autonomous Region 530021, PR 
      China.
FAU - Wei, Zhu-Xin
AU  - Wei ZX
AD  - Department of Radiotherapy, First Affiliated Hospital of Guangxi Medical 
      University, 6 Shuangyong Rd, Nanning, Guangxi Zhuang Autonomous Region 530021, PR 
      China. Electronic address: weizhuxin@stu.gxmu.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20211223
PL  - Germany
TA  - Pathol Res Pract
JT  - Pathology, research and practice
JID - 7806109
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (S-Phase Kinase-Associated Proteins)
RN  - 0 (SKP2 protein, human)
RN  - 0 (WDHD1 protein, human)
RN  - 9007-34-5 (Collagen)
SB  - IM
MH  - Cell Cycle
MH  - Cell Proliferation
MH  - Collagen/genetics/metabolism
MH  - DNA-Binding Proteins/genetics/*metabolism
MH  - Databases, Genetic
MH  - Female
MH  - Gene Expression Regulation, Neoplastic
MH  - Gene Regulatory Networks
MH  - Humans
MH  - Laryngeal Neoplasms/genetics/*metabolism/pathology
MH  - Male
MH  - Middle Aged
MH  - Protein Interaction Maps
MH  - S-Phase Kinase-Associated Proteins/genetics/metabolism
MH  - Signal Transduction
MH  - Squamous Cell Carcinoma of Head and Neck/genetics/*metabolism/pathology
OTO - NOTNLM
OT  - High throughput data
OT  - Immunohistochemistry
OT  - Laryngeal squamous cell carcinoma
OT  - Transcription factor
OT  - WD repeat and HMG-box DNA binding protein 1
EDAT- 2022/01/10 06:00
MHDA- 2022/03/22 06:00
CRDT- 2022/01/09 20:50
PHST- 2021/07/18 00:00 [received]
PHST- 2021/12/21 00:00 [revised]
PHST- 2021/12/21 00:00 [accepted]
PHST- 2022/01/10 06:00 [pubmed]
PHST- 2022/03/22 06:00 [medline]
PHST- 2022/01/09 20:50 [entrez]
AID - S0344-0338(21)00412-X [pii]
AID - 10.1016/j.prp.2021.153751 [doi]
PST - ppublish
SO  - Pathol Res Pract. 2022 Feb;230:153751. doi: 10.1016/j.prp.2021.153751. Epub 2021 
      Dec 23.