PMID- 35003292
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220111
IS  - 1741-427X (Print)
IS  - 1741-4288 (Electronic)
IS  - 1741-427X (Linking)
VI  - 2021
DP  - 2021
TI  - Inhibitory Effects of Inonotus obliquus Polysaccharide on Inflammatory Response 
      in Toxoplasma gondii-Infected RAW264.7 Macrophages.
PG  - 2245496
LID - 10.1155/2021/2245496 [doi]
LID - 2245496
AB  - Our previous reports have shown that Inonotus obliquus polysaccharide (IOP) has 
      protective effects against Toxoplasma gondii (T. gondii) infection in vivo. The 
      aim of the present research is to explore the in vitro anti-inflammatory effects 
      of IOP and its mechanism in RAW264.7 macrophages infected by T. gondii. In this 
      study, it is indicated that IOP decreased the excessive secretion of inflammatory 
      cytokines tumor necrosis factor-alpha (TNF-alpha), interferon-gamma (IFN-gamma), interleukin-1beta 
      (IL-1beta), IL-4, and IL-6 in T. gondii-infected RAW264.7 macrophages. IOP 
      effectively suppressed the mRNA expression of these cytokines and chemokines 
      monocyte chemoattractant protein-1 (MCP-1) and macrophage inflammatory protein-1alpha 
      (MIP-1alpha). Moreover, IOP inhibited the phosphorylation of inhibitor kappa B kinase 
      alpha/beta (IKKalpha/beta), inhibitor kappaBalpha (IkappaBalpha), p65 in nuclear factor-kappa B (NF-kappaB) 
      signaling pathway and p38, c-Jun N-terminal kinase (JNK), and extracellular 
      signal-regulated kinase 1/2 (ERK1/2) in mitogen-activated protein kinases (MAPKs) 
      signaling pathway. Meantime, IOP prevented NF-kappaB p65 and c-Jun translocation from 
      the cytoplasm to the nucleus. Further, IOP downregulated the protein expression 
      of toll-like receptor 2 (TLR2) and TLR4 in T. gondii-infected RAW264.7 
      macrophages. The above results suggest that IOP can inhibit the inflammatory 
      response infected with T. gondii via regulating TLR2/TLR4-NF-kappaB/MAPKs pathways 
      and exerting its anti-T. gondii role in vitro.
CI  - Copyright (c) 2021 Kexin Yan et al.
FAU - Yan, Kexin
AU  - Yan K
AUID- ORCID: 0000-0003-1014-3833
AD  - Agricultural College of Yanbian University, Gongyuan Street, Yanji, Jilin 133002, 
      China.
FAU - Zhou, Hongyuan
AU  - Zhou H
AUID- ORCID: 0000-0002-8012-3742
AD  - Agricultural College of Yanbian University, Gongyuan Street, Yanji, Jilin 133002, 
      China.
FAU - Wang, Meng
AU  - Wang M
AUID- ORCID: 0000-0003-1549-5715
AD  - Agricultural College of Yanbian University, Gongyuan Street, Yanji, Jilin 133002, 
      China.
FAU - Li, Haitao
AU  - Li H
AUID- ORCID: 0000-0001-5107-8455
AD  - Agricultural College of Yanbian University, Gongyuan Street, Yanji, Jilin 133002, 
      China.
AD  - Institute of Special Wild Economic Animals and Plants, Chinese Academy of 
      Agricultural Sciences, Juye Street, Changchun, Jilin 132109, China.
FAU - Sang, Rui
AU  - Sang R
AUID- ORCID: 0000-0002-8402-1110
AD  - Agricultural College of Yanbian University, Gongyuan Street, Yanji, Jilin 133002, 
      China.
FAU - Ge, Bingjie
AU  - Ge B
AUID- ORCID: 0000-0001-9052-1800
AD  - Agricultural College of Yanbian University, Gongyuan Street, Yanji, Jilin 133002, 
      China.
FAU - Zhao, Xin
AU  - Zhao X
AUID- ORCID: 0000-0002-1739-0901
AD  - Agricultural College of Yanbian University, Gongyuan Street, Yanji, Jilin 133002, 
      China.
FAU - Li, Chunting
AU  - Li C
AUID- ORCID: 0000-0001-5691-7430
AD  - Agricultural College of Yanbian University, Gongyuan Street, Yanji, Jilin 133002, 
      China.
FAU - Wang, Wei
AU  - Wang W
AUID- ORCID: 0000-0001-9226-6532
AD  - Agricultural College of Yanbian University, Gongyuan Street, Yanji, Jilin 133002, 
      China.
FAU - Zhang, Xuemei
AU  - Zhang X
AUID- ORCID: 0000-0003-0677-4691
AD  - Agricultural College of Yanbian University, Gongyuan Street, Yanji, Jilin 133002, 
      China.
AD  - Engineering Research Center of North-East Cold Region Beef Cattle Science & 
      Technology Innovation, Ministry of Education, Yanbian University, Gongyuan 
      Street, Yanji, Jilin 133002, China.
LA  - eng
PT  - Journal Article
DEP - 20211229
PL  - United States
TA  - Evid Based Complement Alternat Med
JT  - Evidence-based complementary and alternative medicine : eCAM
JID - 101215021
PMC - PMC8731277
COIS- The authors confirm that there are no conflicts of interest.
EDAT- 2022/01/11 06:00
MHDA- 2022/01/11 06:01
PMCR- 2021/12/29
CRDT- 2022/01/10 09:12
PHST- 2021/10/13 00:00 [received]
PHST- 2021/12/15 00:00 [accepted]
PHST- 2022/01/10 09:12 [entrez]
PHST- 2022/01/11 06:00 [pubmed]
PHST- 2022/01/11 06:01 [medline]
PHST- 2021/12/29 00:00 [pmc-release]
AID - 10.1155/2021/2245496 [doi]
PST - epublish
SO  - Evid Based Complement Alternat Med. 2021 Dec 29;2021:2245496. doi: 
      10.1155/2021/2245496. eCollection 2021.