PMID- 3500397
OWN - NLM
STAT- MEDLINE
DCOM- 19880119
LR  - 20210526
IS  - 0270-7306 (Print)
IS  - 1098-5549 (Electronic)
IS  - 0270-7306 (Linking)
VI  - 7
IP  - 10
DP  - 1987 Oct
TI  - In vitro splicing pathways of pre-mRNAs containing multiple intervening 
      sequences?
PG  - 3428-37
AB  - We analyzed the in vitro splicing pathways of three multi-intervening-sequence 
      (IVS) pre-mRNAs: human beta-globin, which contains two IVSs (K. M. Lang, V. L. 
      van Santen, and R. A. Spritz, EMBO J. 4:1991-1996, 1985); rat alpha-lactalbumin, 
      which contains three IVSs; and murine interleukin-3, which contains four IVSs. We 
      found that there are highly preferred pathways of IVS removal from these 
      multi-IVS pre-mRNAs in vitro. The three IVSs of rat alpha-lactalbumin pre-mRNA 
      were excised sequentially from 5' to 3'; in most molecules, IVS1 was removed 
      first, followed by IVS2 and finally by IVS3. The splicing pathway of 
      interleukin-3 pre-mRNA in vitro was more complex. The four IVSs were excised in a 
      highly preferred temporal order, but the order was not strictly sequential or 
      directional. In most molecules, IVS1 and IVS4 were removed first, either 
      simultaneously or in rapid succession. Subsequently, IVS2 was excised, followed 
      by IVS3. The observed splicing pathways apparently resulted from differences in 
      lag times and maximum excision rates of the different IVSs. We detected no exon 
      skipping during splicing of these transcripts in vitro. These observations have 
      implication for proposed models of splice site selection.
FAU - Lang, K M
AU  - Lang KM
AD  - Department of Medical Genetics, University of Wisconsin, Madison 53706.
FAU - Spritz, R A
AU  - Spritz RA
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Mol Cell Biol
JT  - Molecular and cellular biology
JID - 8109087
RN  - 0 (Interleukin-3)
RN  - 0 (Nucleic Acid Precursors)
RN  - 0 (RNA, Messenger)
RN  - 9004-22-2 (Globins)
RN  - 9013-90-5 (Lactalbumin)
SB  - IM
MH  - Animals
MH  - Globins/genetics
MH  - In Vitro Techniques
MH  - Interleukin-3/*genetics
MH  - Introns
MH  - Lactalbumin/*genetics
MH  - Mice
MH  - Nucleic Acid Precursors/*metabolism
MH  - *RNA Splicing
MH  - RNA, Messenger/*metabolism
MH  - Rats
PMC - PMC367993
EDAT- 1987/10/01 00:00
MHDA- 1987/10/01 00:01
PMCR- 1987/10/01
CRDT- 1987/10/01 00:00
PHST- 1987/10/01 00:00 [pubmed]
PHST- 1987/10/01 00:01 [medline]
PHST- 1987/10/01 00:00 [entrez]
PHST- 1987/10/01 00:00 [pmc-release]
AID - 10.1128/mcb.7.10.3428-3437.1987 [doi]
PST - ppublish
SO  - Mol Cell Biol. 1987 Oct;7(10):3428-37. doi: 10.1128/mcb.7.10.3428-3437.1987.